Muhammad Ashraf

ORCID: 0009-0004-4682-3456
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About
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Research Areas
  • Insect and Pesticide Research
  • Insect Pest Control Strategies
  • Sperm and Testicular Function
  • Reproductive Biology and Fertility
  • Forest Insect Ecology and Management
  • Entomopathogenic Microorganisms in Pest Control
  • Insect Resistance and Genetics
  • RNA Interference and Gene Delivery
  • Essential Oils and Antimicrobial Activity
  • Insect symbiosis and bacterial influences
  • Enzyme-mediated dye degradation
  • Natural product bioactivities and synthesis
  • Chromium effects and bioremediation
  • GABA and Rice Research
  • Antimicrobial Peptides and Activities
  • Plant Gene Expression Analysis
  • Insect Utilization and Effects
  • Antimicrobial agents and applications
  • Dye analysis and toxicity
  • Transgenic Plants and Applications
  • Trace Elements in Health
  • Allelopathy and phytotoxic interactions
  • Bioactive natural compounds
  • Microbial infections and disease research
  • Medicinal Plants and Bioactive Compounds

University of Malakand
2022-2024

Czech University of Life Sciences Prague
2020-2023

Pakistan Institute of Engineering and Applied Sciences
2020

The University of Agriculture, Peshawar
2020

Bahauddin Zakariya University
2017-2018

Nuclear Institute for Agriculture and Biology
2016

Pakistan Science Foundation
2016

Punjab Medical College
2005

Southern Illinois University Carbondale
1982-1984

Bark beetles are destructive forest pests considering their remarkable contribution to depletion. Their association with fungi is useful against the challenges of survival on noxious and nutritionally limited substrate, i.e., conifer tissues. Fungal symbionts help in nutrient acquisition detoxification toxic tree secondary metabolites. Although gut prime location for food digestion detoxification, information available gut-mycobiome bark beetles. The present study screened gut-mycobiont from...

10.3389/fmicb.2020.568853 article EN cc-by Frontiers in Microbiology 2020-09-03

Abstract Bark beetles often serve as forest damaging agents, causing landscape-level mortality. Understanding the biology and ecology of are important for both, gathering knowledge about insects protection. Knowledge bark beetle gut-associated bacteria is one crucial yet surprisingly neglected areas research with European tree-killing beetles. Hence, in this study, we survey gut bacteriome from five Ips non- Scolytinae. Results reveal 69 core bacterial genera among that may perform conserved...

10.1038/s41598-020-75203-5 article EN cc-by Scientific Reports 2020-10-29

This study employs advanced machine learning algorithms Random Forest, Gradient Boosting Machines (GBM), and XGBoost to predict normalized temperatures in major Pakistani cities, focusing on Lahore. Using a comprehensive dataset divided into training testing subsets, model performance was evaluated using Root Mean Square Error (RMSE), Absolute (MAE), R-squared (R²). Results show that excelled with the lowest RMSE (0.0281), MAE (0.0219), highest R² (0.9879), demonstrating superior predictive...

10.71107/j2djza91 article EN 2025-04-30

Azo dyes can be degraded by azoreductases that achieve initial decolorization of the dye molecule through cleavage azo bond. Here we characterized membrane‐bound azoreductase enzyme expressed Shewanella sp. strain IFN4, which is a particularly efficient degrading bacterium. Initial studies showed this bacterium was capable decolorizing wide variety including Reactive Black 5, Acid Red 88, Direct 81, Yellow 19, and Disperse Orange 3. These structurally different were also found to reduced...

10.1002/clen.201501007 article EN CLEAN - Soil Air Water 2016-08-03

The bark beetle, Ips typographus (L.), is a major pest of Norway spruce, Picea abies causing enormous economic losses globally. adult stage the I. has complex life cycle (callow and sclerotized); callow beetles feed ferociously, whereas sclerotized male are more aggressive pioneers in establishing new colonies. We conducted comparative proteomics study to understand female digestion detoxification processes beetles. Proteome profiling was performed using high-throughput liquid...

10.3389/fpls.2023.1157455 article EN cc-by Frontiers in Plant Science 2023-11-21

The emergence of multidrug-resistant (MDR) pathogens is a major problem in the therapeutic management infectious diseases. Among bacterial resistance mechanisms development an enveloped protein and polysaccharide-hydrated matrix called biofilm. Polyphenolics have demonstrated beneficial antibacterial effects. Phenolic compounds mediate their antibiofilm effects via disruption membrane, deprivation substrate, binding, binding to adhesion complex, viral fusion blockage interactions with...

10.2217/fmb-2023-0175 article EN Future Microbiology 2024-02-01

10.1016/0006-291x(83)91589-9 article EN Biochemical and Biophysical Research Communications 1983-07-01

Properties of (Ca2+ + Mg2+) adenosine triphosphatase (ATPase) in plasma membranes from boar epididymal spermatozoa are described. Enzyme activity is optimum at high pH and has a affinity for Ca2+. It not inhibited by the calmodulin antagonist trifluoperazine (TFP), but it low concentrations Plasma membrane vesicles obtained hypotonic lysis intact sperm [mixed inside-out (IOV) right side-out (ROV) vesicles] transport 45Ca2+ presence oxalate. Similar to Ca2+-stimulated Mg ATPase activity,...

10.1095/biolreprod31.5.1061 article EN Biology of Reproduction 1984-12-01

This study is the first report about isolation, purification and optimization of lipase from Bacillus glycinifermentans. In this study, glycinifermentansMK-840989 was isolated a local petrol pump. The bacterium showed lipolytic zones 0.19cm, 0.044cm, 0.28cm on peptone yeast agar, olive oil hydrolysis agar chromogenic plate respectively. B. glycinifermentans also produced an extracellular (55.1µmol/ml). preferred acidic environment (pH 5) for growing optimally at 80˚C when medium supplemented...

10.15406/mojes.2020.05.00181 article EN MOJ Ecology & Environmental Sciences 2020-04-30

Abstract Helicoverpa armigera , a polyphagous insect of crops and vegetables, is acquiring resistance against many commercial insecticides. The present study shows variations in the activity two detoxification enzymes, namely esterase glutathione S‐transferase (GST), H. after exposure to different isolates entomopathogenic fungi. After treatment larvae with (Day 0), samples were collected on three days (Days 3, 5 7) for enzyme analysis. High GST was found hemolymph, intestine fat bodies...

10.1111/1748-5967.12284 article EN Entomological Research 2018-03-23

Abstract Textile wastewater contains huge quantities of nitrogen (N)‐containing azo‐dyes. Irrigation crops with such adds toxic dyes into our healthy soils. One the ways to prevent their entry soils could be these waters after dyes' biodegradation. Therefore, present study was conducted evaluate impact textile on wheat growth, dye degradation efficiency bacteria‐fungi consortium, and alleviation toxicity in by treatment microbial consortium. Among dyes, Red‐S3B (3.19% N) found most...

10.1002/clen.201900305 article EN CLEAN - Soil Air Water 2020-02-19

10.57239/pjlss-2024-22.2.00598 article EN Pakistan Journal of Life and Social Sciences (PJLSS) 2024-01-01

Objective The browning phenomenon in fruits, particularly apples, is a result of various enzymatic reactions, with polyphenol oxidases (PPOs) being the primary culprits. These enzymes catalyze oxidation phenolic compounds presence oxygen, leading to production brown pigments, and compromising quality, texture, appearance fruits. Aims This study aimed investigate methods for mitigating apples by targeting activity PPO through application chemical treatments. Given adverse impact on sensory...

10.17140/aftnsoj-9-182 article EN Advanced in Food Technology and Nutritional Sciences - Open Journal 2023-12-27

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at glance that was extracted from about 200 leading journals. To access Abstract, please click on HTML or PDF.

10.1002/chin.200630196 article EN ChemInform 2006-07-05

Zinc is an essential trace mineral. It considered a growth factor as it isthe metallic component of enzymes DNA polymerase, dependent RNA polymerase and thymidine kinase.Objective: To find out the relationship maternal serum zinc levels with birth weight babies. Period: January1989 to May, 1991. Setting: Lady Wellingdon Hospital Lahore, King Edward College, Lahore MetallurgyDepartment PCSIR Laboratories, Lahore. Design: Prospective study Patients & methods: Among 216 registeredpregnant...

10.29309/tpmj/2005.12.03.5133 article EN The Professional Medical Journal 2005-09-08
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