Oxidizing agents are powerful activators of factors responsible for the transcriptional activation cytokine-encoding genes involved in tissue injury. In this study we show evidence that STAT3 is a transcription factor whose activity modulated by H₂O₂ human lymphocytes, which endogenous catalase had previously been inhibited. H₂O₂-induced nuclear translocation to form sequence-specific DNA-bound complexes was evidenced immunoblotting fractions and...

Abstract The possible presence of Pneumocystis among healthy adults was examined by detecting jirovecii–specific DNA in prospectively obtained oropharyngeal wash samples from 50 persons without underlying lung disease or immunosuppression. carriage, defined nested polymerase chain reaction 2 independent analyses plus successful mitochondrial large subunit ribosomal RNA typing direct sequencing, found 20% cases. All carriers were asymptomatic, anti-HIV negative, and had normal total...

To describe what is, to our knowledge, the first nosocomial outbreak of infection with pan-drug-resistant (including colistin-resistant) Acinetobacter baumannii, determine risk factors associated these types infections, and their clinical impact.Nested case-control cohort study a clinical-microbiological study.A 1,521-bed tertiary care university hospital in Seville, Spain.Case patients were inpatients who had A. baumannii isolate recovered from or surveillance sample obtained at least 48...

We describe here a specific calcineurin activity in neutrophil lysates, which is dependent on Ca2+, inhibited by trifluoroperazine, and insensitive to okadaic acid. Immunoblotting experiments using antiserum recognized both the A B chains of calcineurin. Neutrophils treated with cyclosporin or FK 506 showed dose-dependent inhibition activity. The effect oxidant compounds was also investigated. hydrogen peroxide (H2O2), where catalase aminotriazole, exhibited However, addition reducing agents...

Abstract Background Acinetobacter baumannii causes frequently nosocomial infections worldwide. Its ability to survive on dry surfaces facilitates its spread and the persistence of endemic situations, especially in intensive care units (ICUs). The objective this paper is describe a multicomponent intervention program designed control hyperendemic multidrug-resistant A. (MDR-Ab) characterize impact. Methods Design: Quasi-experimental study based open cohorts. Setting: Public tertiary referral...

A currently ongoing randomized trial has revealed that the Mediterranean diet, rich in virgin olive oil or nuts, reduces systolic blood pressure high-risk cardiovascular patients. Here, we present a structural substudy to assess effect of Mediterranean-style diet supplemented with nuts on erythrocyte membrane properties 36 hypertensive participants after 1 year intervention. Erythrocyte lipid composition, reconstituted membranes, and serum concentrations inflammatory markers are reported....

The influence of body mass index (BMI) on oral cancer risk was evaluated in 375 incident cases and age–gender-matched hospital-based controls. Low BMIs at diagnosis 2 years before were associated with significantly elevated odds ratios (OR for BMI ⩽22 vs >26 kg m−2; 3.64; 95% confidence interval, CI: 2.27–5.82 3.31; 2.04–5.39, respectively). association low BMI, however, tended to be weaker nonsignificant among never smokers drinkers.

It has been demonstrated that neutrophils from healthy donors or patients with inflammatory disorders can bind immunoglobulin (Ig) E proteins through binding to Mac-2/epsilon bp. Functional responses allergens were assessed by measuring the respiratory burst and intracellular Ca2+ levels, of was flow cytometry analysis fluorescence microscopy. In this article, we demonstrate sensitized specific (from allergic patients), but not donors, are sensitive same type as those produce clinical...

Abstract It has been demonstrated that neutrophils are responsible for the release of large amounts inflammatory chemokine interleukin-8 (IL-8), associated with inflammation. To further define mechanisms implicated, we have analyzed response human from allergic patients to specific antigens or challenge anti-immunoglobulin (Ig)E antibodies. Neutrophils showed a dose- and time-dependent production IL-8. The cytokine was parallel expression IL-8 mRNA by polymerase chain reaction. This...

Mitochondrial fatty acid β-oxidation disorders (FAOD) are main targets for newborn screening (NBS) programs, which excellent data sources accurate estimations of disease birth prevalence. Epidemiological is key importance the understanding natural history as well to define more effective public health strategies. In order estimate FAOD prevalence in Iberia, authors collected from six NBS programs Portugal and Spain, encompassing than 1.6 million newborns by tandem mass spectrometry (MS/MS),...

The multiple endocrine neoplasia type 2A (MEN2A) is a monogenic disorder characterized by an autosomal dominant pattern of inheritance which high risk medullary thyroid carcinoma in all mutation carriers. Although this classified as rare disease, the patients affected have low life quality and very expensive continuous treatment. At present, MEN2A diagnosed gene sequencing after birth, thus trying to start early treatment reduction morbidity mortality. We first evaluated presence (C634Y)...

Insulin resistance has been strongly associated with the attainment of sustained viral response (SVR) in hepatitis C patients.To determine, a cohort Spanish patients chronic treated peginterferon plus ribavirin (P+R), whether insulin predicts SVR independently interleukin-28B rs12979860 polymorphism.Insulin was measured as [HOMA-IR = (IU/mL)*glucose (mmol/L)/22.5]. Genotype, load and histological fibrosis using Scheuer score were also measured. Binary logistic regression analysis used for...

Cutaneous malignant melanoma (CMM) morbidity and mortality rates have increased over the past several decades all around world, but some developed countries experienced recent declines in from CMM among young middle-aged even a reversal long-term increase deaths attributable to CMM.To describe trends, by age gender, Spain between 1975 1998.Crude, age-adjusted, truncated, cumulative, age-specific potential years of life lost were calculated gender. Age period Poisson regression models fitted...

We describe an outbreak of nosocomial endophthalmitis due to a common source, which was determined be trypan blue solution prepared in the hospital's pharmacy service. assume that viable bacteria probably gained access stock during cooling after autoclaving. The temporal cluster Pseudomonas aeruginosa readily perceived on basis clinical and microbiological findings, exogenous source contamination unequivocally identified by means DNA fingerprinting.

Abstract Background and Aim: Colorectal cancer (CRC) is one of the most prevalent types affecting both men women in developed countries. Clinical molecular evidence suggests that there are multiple biochemical pathways involved its susceptibility, pathogenesis prognosis. Several studies have reported a significant association between incidence CRC type 2 diabetes mellitus (T2DM). However, genes associated with conditions rare. Method: We analyzed CAPN10 gene, T2DM locus, using UCSNP‐43, ‐44,...

The respiratory-burst reaction has been studied in rat peritoneal macrophages of different ages (3, 12 and 24 months) using phorbol 12-myristate 13-acetate (PMA) to stimulate NADPH oxidase. Production O2-. H2O2 decreased with age (about 50 75% respectively); however, no difference oxidase activity was found. NO. production also reduced (40%). Furthermore, a progressive significant decrease the pentose phosphate flux detected as function control PMA-stimulated macrophages. NADPH/NADP+ ratio...

To determine the nature of mechanism by which certain derived ruthenium (Ru) complexes induce regression in tumour growth, we have investigated possibility that this was associated with an increase superoxide anion (O2-. production phagocytic cells, are usually found nodes. Here present evidence a newly synthesized complex, Ru3+-propylene-1, 2-diaminotetra-acetic acid (Ru-PDTA), from Ru and sequestering ligand (PDTA), specifically stimulates O2-. production. This translocation cytosolic...

Different drugs that elevate the cGMP levels inhibit agonist-induced platelet activation. The mechanisms of action probably include inhibition both phospholipase C and increase in intracellular Ca2+ concentration, these effects seem to be mediated by cGMP-dependent protein kinases. However, most studies, cells were preincubated with nitrovasodilators before stimulation. effect preincubation sodium nitroprusside stimulation or simultaneous addition thrombin has been compared. was able without...

Purpose. Wine consumption has been related to a reduced cardiovascular risk. This effect attributed partly the healthier diet of wine drinkers. We compared food habits according alcoholic beverage preference in Mediterranean population. Design. A cross-sectional study large sample participants at high risk for disease. Setting. Primary care centers country, Spain. Participants. total 1675 men aged 55 80 years old and 2150 women 60 who had no documented disease but either diabetes or least...