A5044509253- RNA Research and Splicing
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA modifications and cancer
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Immune cells in cancer
- RNA regulation and disease
- Genetics and Neurodevelopmental Disorders
- Ubiquitin and proteasome pathways
- Cancer-related molecular mechanisms research
- Regulation of Appetite and Obesity
- Cholinesterase and Neurodegenerative Diseases
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Single-cell and spatial transcriptomics
- Neurological Disorders and Treatments
- Adipose Tissue and Metabolism
- Diet and metabolism studies
Max Planck Institute for Multidisciplinary Sciences
2022-2024
University of Göttingen
2022-2024
Universitätsmedizin Göttingen
2022-2024
Alzheimer’s Disease Neuroimaging Initiative
2023
Union Bank of Switzerland
2023
Max Planck Institute of Experimental Medicine
2018-2022
Myelin, the electrically insulating sheath on axons, undergoes dynamic changes over time. However, it is composed of proteins with long lifetimes. This raises question how such a stable structure renewed. Here, we study integrity myelinated tracts after experimentally preventing formation new myelin in CNS adult mice, using an inducible Mbp null allele. Oligodendrocytes survive recombination, continue to express genes, but they fail maintain compacted sheaths. Using 3D electron microscopy...
Human myelin disorders are commonly studied in mouse models. Since both clades evolutionarily diverged approximately 85 million years ago, it is critical to know what extent the protein composition has remained similar. Here, we use quantitative proteomics analyze purified from human white matter and find that relative abundance of structural proteins PLP, MBP, CNP, SEPTIN8 correlates well with C57Bl/6N mice. Conversely, multiple other were identified exclusively or predominantly myelin....
To maintain homeostasis, the body, including brain, reprograms its metabolism in response to altered nutrition or disease. However, consequences of these challenges for energy different brain cell types remain unknown. Here, we generated a proteome atlas major central nervous system (CNS) from young and adult mice, after feeding therapeutically relevant low-carbohydrate, high-fat ketogenic diet (KD) during neuroinflammation. Under steady-state conditions, CNS prefer distinct modes...
Abstract Introduction Extracellular vesicles (EVs) may propagate and modulate Alzheimer's disease (AD) pathology. We aimed to comprehensively characterize the proteome of cerebrospinal fluid (CSF) EVs identify proteins pathways altered in AD. Methods CSF were isolated by ultracentrifugation (Cohort 1) or Vn96 peptide 2) from non‐neurodegenerative controls ( n = 15, 16) AD patients 22, 20, respectively). subjected untargeted quantitative mass spectrometry‐based proteomics. Results validated...
Summary A proper equilibrium of post‐translational protein modifications is essential for normal cell physiology, and alteration in these processes key neurodegenerative disorders such as Alzheimer's disease. Recently, instance, SUMO ylation has been linked to amyloid pathology. In this work, we aimed elucidate the role during aging increased burden vivo using a His 6 ‐ HA 1 knock‐in mouse 5 XFAD model Interestingly, did not observe any levels SUMO1‐conjugation related conjugates remained...
Summary Myelin, the electrically insulating axonal sheath, is composed of lipids and proteins with exceptionally long lifetime. This raises question how myelin function affected by turnover. We have studied integrity myelinated tracts after experimentally preventing formation new in CNS adult mice, using an inducible Mbp null allele. Oligodendrocytes survived recombination, continued expressing genes, but failed to maintain compacted sheaths. Using 3D electron microscopy mass spectrometry...
Abstract The molecules that constitute myelin are critical for the integrity of axon/myelin‐units and thus speed precision impulse propagation. In CNS, protein composition oligodendrocyte‐derived has evolutionarily diverged differs from in PNS. Here, we hypothesized CNS proteome also displays variations within same species. We used quantitative mass spectrometry to compare purified mouse brains at three developmental timepoints, male female mice, four regions. find most structural proteins...
Abstract Human myelin disorders are commonly studied in mouse models. Since both clades evolutionarily diverged approximately 85 million years ago, it is critical to know what extent the protein composition has remained similar. Here we use quantitative proteomics analyze purified from human white matter and find that relative abundance of structural proteins PLP, MBP, CNP SEPTIN8 correlates well with C57Bl/6N- mice. Conversely, multiple other were identified exclusively or predominantly...
ABSTRACT Kaufman oculocerebrofacial syndrome (KOS) is a severe autosomal recessive disorder characterized by intellectual disability, developmental delays, microcephaly and characteristic dysmorphisms. Biallelic mutations of UBE3B , encoding for ubiquitin ligase E3B are causative KOS. In this report, we characterize neuronal functions its murine ortholog Ube3b . We show that regulates dendritic branching in cell-autonomous manner. Moreover, knockout (KO) neurons exhibit increased density...