Abstract Muscle inflammation and fibrosis underlie disuse‐related complications may contribute to impaired muscle recovery in aging. Cellular senescence is an emerging link between inflammation, extracellular matrix (ECM) remodeling poor after disuse. In rodents, metformin has been shown prevent cellular senescence/senescent associated secretory phenotype (SASP), making it a potentially practical therapeutic solution. Thus, the purpose of this study was determine older adults if monotherapy...

Abstract Aging coincides with the progressive loss of muscle mass and strength, increased adiposity, diminished physical function. Accordingly, interventions aimed at improving muscle, metabolic, and/or health are interest to mitigate adverse effects aging. In this study, we tested a stem cell secretome product, which contains extracellular vesicles growth, cytoskeletal remodeling, immunomodulatory factors. We examined 4 weeks 2×/week unilateral intramuscular injections (quadriceps) in...

Abstract Loss of muscle mass and strength after disuse followed by impaired recovery commonly occurs with aging. Metformin (MET) leucine (LEU) individually have shown positive effects in skeletal during atrophy conditions but not been evaluated combination nor tested as a remedy to enhance following The purpose this study was determine if dual treatment metformin (MET + LEU) would prevent disuse‐induced and/or promote aged mice these responses correspond changes satellite cells collagen...

Poor recovery of muscle size and strength with aging coincides a dysregulated macrophage response during the early stages regrowth. Immunomodulation in form ex vivo cytokine (macrophage-colony stimulating factor) or polarized delivery has been demonstrated to improve skeletal regeneration. However, it is unclear if these macrophage-promoting approaches would be effective following disuse aged animals. Here, we isolated bone marrow-derived macrophages from donor mice different ages under...

Objective Inactivity and inflammation are linked to obesity insulin resistance. It was hypothesized that MyD88 (mediates inflammation) knockout from muscle (MusMyD88 −/− ) would prevent, whereas miR146a (MyD88 inhibitor) exacerbate, inactivity‐induced metabolic disturbances. Methods Cre‐control, MusMyD88 , mice were given running wheels for 5 weeks model an active phenotype. Afterward, half placed into a small mouse cage (SMC) restrict movement 8 days. Body composition, ( 3 H)2‐deoxyglucose...

Timely and complete recovery of muscle mass function following a bout physical disuse are critical components returning to normal activities daily living lifestyle. Proper cross talk between the tissue myeloid cells (e.g., macrophages) throughout period from atrophy plays significant role in resolution size function. Chemokine C-C motif ligand 2 (CCL2) has recruiting macrophages during early phase damage. However, importance CCL2 not been defined context recovery. Here, we utilized mouse...

Cheddar cheese is a protein-dense whole food and high in leucine content. However, no information known about the acute blood amino acid kinetics protein anabolic effects skeletal muscle healthy adults. Therefore, we conducted crossover study which men women (n = 24; ~27 years, ~23 kg/m2) consumed (20 g protein) or an isonitrogenous amount of milk. Blood biopsies were taken before during post absorptive period following ingestion. We evaluated circulating essential non-essential acids,...

Metformin is the most commonly prescribed medication to treat diabetes. Emerging evidence suggests that metformin could have off target effects might help promote healthy muscle aging, but these not been thoroughly studied in glucose tolerant older individuals. The purpose of this study was investigate short-term consumption on skeletal mitochondrial bioenergetics adults.

Abstract Aged individuals are at risk to experience slow and incomplete muscle recovery following periods of disuse atrophy. While several therapies have been employed mitigate mass loss during improve recovery, few proven effective both. Therefore, the purpose this study was examine effectiveness a uniquely developed secretome product (STEM) on aged skeletal function recovery. (22 months) male C57BL/6 were divided into PBS or STEM treatment ( n = 30). Mice within each assigned either...

Muscle progenitor cells (MPCs) in aged muscle exhibit impaired activation into proliferating myoblasts, thereby impairing fusion and changes secreted factors. The antihyperglycemic drug metformin, currently studied as a candidate antiaging therapy, may have potential to promote function of MPCs. We evaluated the impact 2 wk metformin ingestion on primary myoblast measured vitro after being extracted from biopsies older adult participants. MPCs were isolated community-dwelling (4 male/4...

Abstract Periods of inactivity experienced by older adults induce nutrient anabolic resistance creating a cascade skeletal muscle transcriptional and translational aberrations contributing to dysfunction. The purpose this study was identify how alters leucine-stimulated translation molecules pathways within the adults. We performed ribosomal profiling alongside RNA sequencing from biopsies taken (n = 8; ~72 years; 6 F/2 M) in response leucine bolus before (Active) after (Reduced Activity) 2...

We demonstrate that early life disuse resulted in less grip strength adulthood. Analysis of muscle composition demonstrated no loss whole or myofiber size indicating lower quality akin to premature aging. This poor was characterized by altered macrophages and extracellular matrix area. intriguing correlations between this also area noncontractile tissue the muscle.

Aging coincides with the accumulation of senescent cells within skeletal muscle that produce inflammatory products, known as senescence-associated secretory phenotype, but relationship to atrophy is unclear. Previously, we found a metformin + leucine (MET+LEU) treatment had synergistic effects in aged mice improve structure and function during disuse atrophy. Therefore, study’s purpose was determine mechanisms by which MET+LEU exhibits protection vitro if this occurs through cellular...

Obesity is often accompanied by heightened circulating and tissue inflammation along with an increase in sphingolipids (e.g., ceramides) metabolically active insulin-sensitive organs. Whey protein isolate (WPI) has been shown to decrease insulin sensitivity when given during a high-fat diet (HFD) intervention rodents. The whey bioactive peptide glycomacropeptide (GMP) also linked having anti-inflammatory properties regulating lipogenesis. Therefore, the purpose of study was determine effect...

Introduction: A hallmark of aging is poor muscle recovery following disuse atrophy. Efficacious strategies to enhance atrophy in are non-existent. Prior exercise training could result favorable morphological and cellular adaptations that may promote aging. Here, we characterized the impact on skeletal inflammatory metabolic profiles remodeling function, together with femoral artery reactivity prior from aged male mice. We hypothesized 12 weeks treadmill mice would improve at baseline during...

We determined that skeletal muscle-specific AMPKα knockout (KO) mice display functional, fibrotic, and transcriptional alterations before during muscle disuse atrophy. also observed KO drives fibrosis pathways related to cellular senescence continues the hindlimb unloading period.

Skeletal muscle insulin resistance is an undesirable outcome during periods of physical inactivity in older adults, which can lead to a variety metabolic diseases. Insulin sensitive tissue (e.g., liver, and skeletal muscle) derived ceramides are well known promote resistance. Evidence suggests that metformin regulate ceramide accumulation. Therefore, we hypothesized treatment short-term period bed rest male female adults would reduce accumulation prevent Our study design consisted (66 ± 4.5...

PURPOSE: To evaluate the effects of whey protein supplementation in adolescent soccer players on body composition and performance over a 10-week competitive season double-blind, randomized controlled trial. METHODS: Adolescents athletes (n = 22; 59% female, age: 15.6 ± 0.2; BMI percentile: 55.9 6.2%) underwent following assessments at pre- post-intervention: fat mass fat-free (via air displacement plethysmography), timed sprint 30-yard dash), estimated VO2max (using 1.5-mile run protocol),...

Einleitung Bei der kongenitalen Zwerchfellhernie (CHD) kommt es aufgrund einer Lücke im Zwerchfell zur Verlagerung von Abdominalorganen in den Thoraxraum. Die Inzidenz liegt bei ca. 1: 2500 bis 4000 Lebendgeburten. Mortalität 30–60%. Ziel dieser Studie war es, die Häufigkeit Begleitfehlbildungen (BF) und deren Detektionsraten mittels Ultraschall zu erfassen. Sowie Einfluss auf das postnatale Outcome untersuchen. Material Methoden Zwischen 2001 2008 wurden 362 Fälle mit CHD Perinatalzentrum...

Einleitung: Die kongenitale Zwerchfellhernie findet man bei circa 1: 2500 Lebendgeburten vor. Durch den Defekt des Zwerchfells kommt es zur Herniation abdominaler Organe. Hierdurch kann deutlichen Verminderung Lungenvolumens und pulmonalen Hypoplasie kommen. In letzten Jahren hat sich die Berechnung Restlungenvolumens fetaler mittels Kernspintomografie als Goldstandard etabliert. Eine zuverlässige Bestimmung ist ein guter prädiktiver Wert für klinischen Schweregrad postpartal. Ziel dieser...

Einleitung: Als Holoprosenzephalie bezeichnet man eine unvollständige oder fehlende Differenzierung des Prosenzephalons. Das Spektrum reicht vom partiellen bis hin zum völligen Fehlen der Spaltung in zwei Hirnhemisphären und Seitenventrikel. Begleitet wird diese Fehlbildung von sowohl fazialen als auch extrafazialen Anomalien. Die Ätiologie ist nicht vollständig bekannt. Prognose ungünstig.

Einleitung Es existieren zahlreiche Formeln zur sonogr. Gewichtsschätzung beim Feten. Neben den üblichen Längen- und Umfangmessungen berücksichtigen neuere maternale Parameter oder das Fetalgeschlecht. In dieser Studie wurden verschiedene auf ihre Genauigkeit in Hinsicht Geburtsgewicht verglichen.