A5089006886- Lipid Membrane Structure and Behavior
- 3D Printing in Biomedical Research
- Enzyme Structure and Function
- Algal biology and biofuel production
- Carbohydrate Chemistry and Synthesis
- Receptor Mechanisms and Signaling
- earthquake and tectonic studies
- Electrochemical sensors and biosensors
- Hydraulic Fracturing and Reservoir Analysis
- Protein Structure and Dynamics
- Cellular Mechanics and Interactions
- Ion channel regulation and function
- Electron Spin Resonance Studies
- Hydrocarbon exploration and reservoir analysis
- Spectroscopy and Quantum Chemical Studies
- Electrochemical Analysis and Applications
- Virus-based gene therapy research
- Antimicrobial Peptides and Activities
- Viral gastroenteritis research and epidemiology
- Supramolecular Self-Assembly in Materials
- Advanced Proteomics Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Drug Transport and Resistance Mechanisms
- Glycosylation and Glycoproteins Research
- Pancreatic function and diabetes
Fraunhofer Institute for Cell Therapy and Immunology
2021-2024
Technische Universität Berlin
2021-2024
Heidelberg University
2013-2015
GFZ Helmholtz Centre for Geosciences
2000
As a defined model of outer membranes gram negative bacteria, we investigated the interaction monolayers lipopolysacchrides from Salmonella enterica rough strains R90 (LPS Ra) with natural and synthetic peptides. The fine structures perpendicular to membrane plane ion distribution near interface were determined by specular x-ray reflectivity (XRR) grazing-incidence fluorescence (GIXF) in presence absence divalent cations. unique combination XRR GIXF allows for quantitative identification...
Investigating cohesive tissue sheets in controlled cultures still poses a challenge since the complex intercellular interactions are difficult to mimic vitro models. We used supported lipid membranes functionalized by adhesive part of extracellular domain cell adhesion molecule cadherin-11 for immobilization pluripotent sheets, animal cap isolated from Xenopus laevis blastula stage embryos. Cadherin-11 was bound via histidine tag with chelator head groups. In first step, quantitative...
Here, we present a solid-supported membrane (SSM)-based electrophysiological approach to study sugar binding and Na+/glucose cotransport by SGLT1 in vesicles. SSM-based electrophysiology delivers cumulative real-time current readout from numerous proteins simultaneously using gold-coated sensor chip. In contrast conventional techniques, which mainly operate with voltage steps, currents are triggered or sodium addition. Sugar concentration jumps the presence of lead transport between 5 10 nA....
We have demonstrated that the complementary combination of grazing incidence X-ray fluorescence (GIXF) with specular reflectivity (XRR) can be used to quantitatively determine density profiles Ni(2)(+) ions complexed chelator headgroups as well S atoms in recombinant proteins anchored lipid monolayers at air/water interface. First, we prepared phospholipid incorporating anchors different molar fractions The fine-structures perpendicular global plane were characterized by XRR presence ions,...
The mechanical properties of multilayer stacks Gb3 glycolipid that play key roles in metabolic disorders (Fabry disease) were determined quantitatively by using specular and off-specular neutron scattering. Because the geometry membrane deposited on planar substrates, scattered intensity profile was analyzed a 2D reciprocal space map as function in-plane out-of-plane scattering vector components. two principal parameters membranes, namely, bending rigidity compression modulus, can be...
Fracture systems and fluid pathways must be analysed in order to understand the dynamical processes upper crust. Various deterministic as well stochastic fracture networks depth section of Franconian Lineament (6900 7140 m), which appears a brittle ductile shear zone prominent seismic reflector, were modelled simulate hydraulic situation at two boreholes Continental Deep Drilling Program (KTB). They led estimations permeability crystalline rock. The geometrical parameters fractures, such...
The human Peptide Transporter 1 (hPepT1) is known for its broad substrate specificity and ability to transport (pro-)drugs. Here, we present an in-depth comprehensive study of hPepT1 interactions with various substrates via solid supported membrane-based electrophysiology (SSME). Using hPepT1-containing vesicles, could not identify any peptide induced pre-steady-state currents, indicating that the recorded peak currents reflect steady-state transport. Electrogenic co-transport...
Chinese hamster ovary (CHO) cells are critical in biopharmaceutical production due to their scalability and capacity for human-like post-translational modifications. However, toxic proteins membrane often difficult-to-express living cells. Alternatively, cell-free protein synthesis can be employed. This study explores innovative strategies enhancing the of challenging through modification CHO by investigating both, cell-based approaches. A major result our involves integration a mutant eIF2...
The major aim of this thesis is to create a novel in vitro model substrate for the controlled cell differentiation pluripotent tissue sheets by fine-tuning balance between cell-cell (in plane) and cell-matrix (out interactions. As culture explants, planar lipid membranes deposited on solid substrates (supported membranes) were functionalized with extracellular domain Xenopus cadherin-11 (Xcad-11), expressed embryos during neural crest (NCC) differentiation. In Chapter 4, quantitative...