A5044015927- Meat and Animal Product Quality
- Protein Hydrolysis and Bioactive Peptides
- Aquaculture Nutrition and Growth
- Antimicrobial Peptides and Activities
- Proteins in Food Systems
- Biochemical effects in animals
- Marine Toxins and Detection Methods
- Probiotics and Fermented Foods
- Muscle metabolism and nutrition
- Marine Biology and Environmental Chemistry
- Food Safety and Hygiene
- Advanced Chemical Sensor Technologies
- Aquaculture disease management and microbiota
- Lipid Membrane Structure and Behavior
- Analytical chemistry methods development
- Salmonella and Campylobacter epidemiology
- Biochemical Analysis and Sensing Techniques
- Essential Oils and Antimicrobial Activity
- Fermentation and Sensory Analysis
- Analytical Chemistry and Chromatography
- Physiological and biochemical adaptations
- Insect Utilization and Effects
- Animal Nutrition and Physiology
- Phytase and its Applications
- Horticultural and Viticultural Research
East Surrey Hospital
2023
Dalhousie University
2009-2021
Purdue University West Lafayette
2016
St. Francis Xavier University
2003
University of Guelph
1974-2003
Technical University of Nova Scotia
1984-2001
Graduate School Experimental Plant Sciences
1997
Danish Ministry of Food, Agriculture and Fisheries
1996
Technical University of Denmark
1996
Brigham and Women's Hospital
1991
A nano-encapsulation strategy for a chitosan-coated liposomal oral delivery system using milk fat globule membrane (MFGM) phospholipids the entrapment of antidiabetic peptides prepared from Atlantic salmon (Salmo salar) protein hydrolysates (SPH) was developed. The size, zeta potential, efficiency, stability during freeze-drying freeze thawing, and long-term storage abilities nano-capsules were investigated as function concentrations phospholipid chitosan coating material. Chitosan greatly...
Abstract Canadian seafood quality has traditionally been examined by the subjective parameters of odor, color, flavor and appearance. Also, products have most often marketed on a one price system; high mediocre being sold at same price. A principal advantage objective assessment is ability to assign meaningful numerical scores raw material finished product, thus permitting adjustment market prices providing cash incentive fishermen, processors retailers maintain standards. Development rapid,...
Thermal denaturation and aggregation abilities of salmon myofibrils myosin were studied measuring turbidity, intrinsic fluorescence, 8-anilino-1-naphtalene sulfonic acid binding, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide cross-linking. The thermal behaviors protein preparation from white red muscles compared, the relationship with gelation properties is discussed. low ability muscle proteins was related to a limited extent upon heating. These seemed be carried by molecules as similar...
The inhibitory effect of the cationic peptide protamine on Listeria monocytogenes, Escherichia coli, and Shewanella putrefaciens has been studied in detail. addition (10 to 1,000 micrograms/ml) resulted inhibition oxygen consumption after less than 1 min loss intracellular carboxyfluorescein ATP 2 5 min. Maximum antibacterial activity was reached at alkaline pH absence divalent cations. efficient permeabilization cell envelopes both gram-positive gram-negative bacteria suggests that causes a...
ABSTRACT Cross‐linking of myofibrillar proteins extracted from cod ( Gadus morhua) , herring Clupea harengus ) and silver hake Merluccius bilinearis was studied in 0.6M NaCl, pH 6.5 at 40°C evaluated turbidimetrically by SDS polyacrylamide gel electrophoresis coupled with l‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide as a zero‐length crosslinker. Turbidities heat‐treated myofibril/myosin solutions were significantly higher than those herring. Electrophoretic results showed that the myosin...
Cod muscle has a balanced protein profile that contains potentially bioactive amino acid sequences. However, there is limited information on release of these peptides from the parent proteins and their ability to modulate mammalian blood pressure.The aim this study was generate cod antihypertensive with potent in vitro inhibitory effects against angiotensin-converting enzyme (ACE) renin. The most active were then tested for systolic pressure (SBP)-reducing spontaneously hypertensive rats...
Impedimetric measurements were used to assay the antibacterial effect of protamine. A good linear correlation between impedance detection time and initial cell counts was obtained ( r = 0.99, n 2). As basic peptides may cause clumping cells, this curve when estimating number after protamine treatment, rather than colony counts. Protamine from salmon killed growing Gram‐positive bacteria significantly inhibited growth Gram‐negative in Tryptone Soy Broth (TSB) at 25°C. In general more...
Protamine is a cationic antimicrobial peptide, which inhibits or kills number of Gram-negative bacteria, including Pseudomonas aeruginosa and Escherichia coli. Electrostatic interactions between the outer leaflet membrane protamine are thought to be important for effect. We hypothesized that divalent ions would compete with binding charged O-sidechain liposaccharide expel from O-sidechains. Experimentally it was shown increasing concentrations cations (Ca2+ Mg2+) reduced effect on P. PA01 E....
To assess right and left ventricular systolic function in normal human fetuses by cross sectional Doppler echocardiography to calculate the force developed myocardial shortening.Cross echocardiographic images of aorta pulmonary arteries were obtained prospectively order measure great vessel diameters their areas. velocity signals recorded from proximal artery digitised obtain peak velocity, acceleration time, flow time integral during acceleration, for whole ejection. Right development was...
Grazing incidence x-ray scattering techniques and Monte Carlo (MC) simulations are combined to reveal the influence of molecular structure (genetic mutation) divalent cations on survival gram negative bacteria against cationic peptides such as protamine. The former yields detailed structures bacterial lipopolysaccharide (LPS) membranes with minimized radiation damages, while minimal computer model based linearized Poisson-Boltzmann theory allows for simulation conformational changes...
As a defined model of outer membranes gram negative bacteria, we investigated the interaction monolayers lipopolysacchrides from Salmonella enterica rough strains R90 (LPS Ra) with natural and synthetic peptides. The fine structures perpendicular to membrane plane ion distribution near interface were determined by specular x-ray reflectivity (XRR) grazing-incidence fluorescence (GIXF) in presence absence divalent cations. unique combination XRR GIXF allows for quantitative identification...
Fusarium graminearum is a very destructive fungal pathogen that leads to head blight (FHB) in wheat, disease which costs growers millions of dollars annually both crop losses and remediation efforts. Current countermeasures include the deployment wheat varieties with some resistance FHB conjunction timed fungicide treatments. In this article, we introduce based on thymol, naturally occurring plant phenolic derived from essential oils. To overcome hydrophobicity thymol active was incorporated...
ABSTRACT Rigor mortis of cultivated Atlantic salmon ( Salmo salar ) began to set in 8h after death and was fully resolved 60–70h during storage at 0°C. Maximum muscle contraction observed 24–30h death. ATP content decreased from 7.25 0.14 0.09 μmol/g fish pre‐rigor in‐rigor post‐rigor state. The inosine hypoxanthine contents increased 0 1.20 4.06 μmol/ g 0.08 0.33 0.84 60h storage, respectively, Postmortem changes affected salt uptake. equilibrium concentrations pre‐rigor, were 0.53, 0.66...