A5005504016- Diabetes, Cardiovascular Risks, and Lipoproteins
- Peroxisome Proliferator-Activated Receptors
- Lipoproteins and Cardiovascular Health
- Lipid metabolism and biosynthesis
- Cholesterol and Lipid Metabolism
- Lipid metabolism and disorders
- Cancer, Lipids, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Fatty Acid Research and Health
- Glycosylation and Glycoproteins Research
- Cellular transport and secretion
- Eicosanoids and Hypertension Pharmacology
- Carbohydrate Chemistry and Synthesis
- Adipokines, Inflammation, and Metabolic Diseases
- Ubiquitin and proteasome pathways
- Enzyme Catalysis and Immobilization
- RNA and protein synthesis mechanisms
- Nuclear Structure and Function
- Pancreatic function and diabetes
- Fluorine in Organic Chemistry
- RNA modifications and cancer
- Liver Disease Diagnosis and Treatment
- Adipose Tissue and Metabolism
- Biopolymer Synthesis and Applications
- Blood properties and coagulation
Dalhousie University
2006-2021
Lancaster City Council
2007
University of Ottawa
1996-2002
Queen's University
1998
Bristol-Myers Squibb (Germany)
1996
University of Alberta
1994-1995
University of British Columbia
1982-1994
Shaughnessy Hospital
1986-1988
Oklahoma Medical Research Foundation
1988
University of Victoria
1984
Intracellular degradation of newly synthesized apolipoprotein (apo) B can occur at every stage the secretory pathway, from protein translation, polypeptide translocation across membrane endoplasmic reticulum (ER), to vesicular transport. The prevalence apoB each varies in different hepatic cell systems examined. Proteolysis nascent be catalyzed by ubiquitin-proteasome system cytosol, and probably unidentified ER resident proteases as well. Cytosolic lumenal molecular chaperones that...
We studied the role of microsomal triglyceride transfer protein (MTP) in synthesis, secretion, and cotranslational degradation apolipoprotein (apo) B using nonhepatic COS-7 cells that expressed C-terminally truncated forms apoB (from apoB15 to apoB94) with or without large subunit human MTP. With exception apoB18, secretion all was stimulated by expression MTP, even though a small amount short (≤apoB48) could be secreted transfected alone. The majority protein, including apoB72 apoB94, as...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConformations of cis- and trans-4-fluoro-Yl-proline in aqueous solutionJ. T. Gerig R. S. McLeodCite this: J. Am. Chem. Soc. 1973, 95, 17, 5725–5729Publication Date (Print):August 1, 1973Publication History Published online1 May 2002Published inissue 1 August 1973https://pubs.acs.org/doi/10.1021/ja00798a046https://doi.org/10.1021/ja00798a046research-articleACS PublicationsRequest reuse permissionsArticle Views275Altmetric-Citations63LEARN ABOUT...
The requirement of the activity microsomal triglyceride transfer protein (MTP) for very low density lipoprotein (VLDL) secretion was determined using McA-RH7777 cells stably transfected with human apoB48 (hB48). Secretion VLDL containing hB48 (hB48-VLDL) by induced exogenous oleate (0.4 mm), and oleate-dependent selectively inhibited brefeldin A (0.2 μg/ml). Two protocols were used to determine effect MTP inhibition on secretion. In first protocol, cell lipid labeled radioactive amino acids...
Canldiapolipoprotein (apo) B plays an obligatory role in the assembly and secretion of hepatic triglyceride-rich lipoproteins.Investigation truncated human apoB variants associated with hypobetalipoproteinemia has suggested that both size apoB-containing lipoproteins may be reduced by carboxyl-terminal truncation.To examine carboxyl terminus lipoproteins, we have generated rat hepatoma McA-RH7777 cells synthesize secrete full- length apoBl00 forms B94, B88, B80, B72, B60.In resulting...
Circulating human pancreatic polypeptide (HPP) was studied in 16 subjects during two 24-h test periods, one fasting and another eating meals at 0830, 1230, 1730. In six the tests were repeated with propantheline administration. Blood sampled every 30 min; additional blood samples drawn 15 45 min postprandially. Mean HPP concentrations increased gradually day 13 out of subjects, reaching an average peak 2100. By 0200 mean returned to initial concentration. Propantheline eliminated this...
Apolipoprotein B-100 (apoB-100) is degraded by endoplasmic reticulum-associated degradation (ERAD) when lipid availability limits assembly of VLDLs. The ubiquitin ligase gp78 and the AAA-ATPase p97 have been implicated in proteasomal apoB-100. To study relationship between ERAD VLDL assembly, we used small interfering RNA (siRNA) to reduce expression HepG2 cells. Reduction decreased apoB-100 ubiquitination cytosolic apoB-ubiquitin conjugates. Radiolabeling studies revealed that knockdown...
We studied the structural requirements of apolipoprotein (apo) B for assembly very low density lipoproteins (VLDL) using rat hepatoma McA-RH7777 cells expressing human apoB (h-apoB). Recombinant h-apoB48, like endogenous apoB48 (r-apoB48), was secreted as VLDL in addition to high (HDL) by transfected cells, indicating that N-terminal 48% contains sequences sufficient assembly. Truncation C terminus h-apo-B48 -B42 or -B37 had little effect on ability assemble VLDL, whereas truncation -B34...
Abstract Oversecretion of apoB and decreased removal apoB-containing lipoproteins by the liver results in hyperapobetalipoproteinemia, which is a risk factor for atherosclerosis. We investigated how dexamethasone, synthetic glucocorticoid, affects synthesis, degradation, secretion apoB-100 apoB-48. Primary rat hepatocytes were incubated with dexamethasone 16 hours. Incorporation [ 35 S]methionine into apoB-48 was increased 36% 50%, respectively, 10 nmol/L despite 28% decrease incorporation...
The adipocyte-derived secretory protein adiponectin functions as an insulin-sensitizing agent. In plasma, exists low, medium, and high molecular weight oligomers. Treatment with trans-10, cis-12 conjugated linoleic acid (t-10, c-12 CLA) reduces levels of well triglyceride (TG) in mice adipocyte cell culture models. aim this study was to determine whether the effects t-10, CLA on TG are mediated through modulation transcription factor peroxisome proliferator-activated receptor γ (PPARγ)....
Treatment of epilepsy or bipolar disorder with valproic acid (VPA) induces weight gain and increased serum levels for the satiety hormone, leptin, through an unidentified mechanism. In this study we tested effects VPA, a short-chain branched fatty (C8:0), on leptin biology metabolism in 3T3-L1 adipocytes. VPA significantly reduced secretion dose-dependent manner. Because accumulation has been hypothesized to block secretion, effect metabolism. Using 14C-radiolabeled found that 14C was mainly...
Abstract Objective: To determine whether adipocyte enhancer binding protein (AEBP) 1, a transcriptional repressor that is down‐regulated during adipogenesis, functions as critical regulator of adipose tissue homeostasis through modulation phosphatase and tensin homolog deleted on chromosome ten (PTEN) tumor suppressor activity mitogen‐activated kinase (MAPK) activation. Research Methods Procedures: We examined AEBP1 physically interacts with PTEN in 3T3‐L1 cells by coimmunoprecipitation...