A5055281543- Diabetes, Cardiovascular Risks, and Lipoproteins
- Lipoproteins and Cardiovascular Health
- Cancer, Lipids, and Metabolism
- Lipid metabolism and disorders
- Cholesterol and Lipid Metabolism
- Peroxisome Proliferator-Activated Receptors
- Liver Disease Diagnosis and Treatment
- Adipokines, Inflammation, and Metabolic Diseases
- Fatty Acid Research and Health
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Metabolism and Genetic Disorders
- Drug Transport and Resistance Mechanisms
- Lipid metabolism and biosynthesis
- Advanced Chemical Physics Studies
- Metabolomics and Mass Spectrometry Studies
- Diet, Metabolism, and Disease
- Inorganic Fluorides and Related Compounds
- Pharmaceutical Economics and Policy
- Malaria Research and Control
- Crystallography and Radiation Phenomena
- Menopause: Health Impacts and Treatments
- Hormonal and reproductive studies
- Rangeland and Wildlife Management
- Cardiovascular Disease and Adiposity
Izaak Walton Killam Health Centre
2023
University of New England
2019-2022
The University of Western Australia
2010-2019
Interactive Flow Studies (United States)
2017
Barrett Technology (United States)
2012-2017
Royal Perth Hospital
2006-2016
Amgen (United States)
2016
Amgen (Switzerland)
2016
University of Washington
1993-2015
University of Otago
2015
Familial hypercholesterolemia is caused by mutations in the LDL receptor gene (Ldlr). Elevated plasma levels result from slower catabolism and a paradoxical lipoprotein overproduction. We explored relationship between presence of secretion hepatocytes both wild-type receptor–deficient mice. Ldlr–/– secreted apoB100 at 3.5-fold higher rate than did hepatocytes. ApoB mRNA abundance, initial apoB synthetic rate, abundance microsomal triglyceride transfer protein 97-kDa subunit not differ cells....
<b><i>Objective:</i></b> To evaluate the efficacy and tolerability of lamotrigine (LTG) for treatment pain in HIV-associated sensory neuropathies. <b><i>Methods:</i></b> In a randomized, double-blind study, patients with distal polyneuropathy (DSP) received LTG or placebo during 7-week dose escalation phase followed by 4-week maintenance phase. Randomization was stratified according to whether not were currently using neurotoxic antiretroviral therapy (ART). <b><i>Results:</i></b> The number...
Triglyceride (TG) enrichment of HDL resulting from cholesteryl ester transfer protein–mediated exchange with TG-rich lipoproteins may enhance the lipolytic transformation and subsequent metabolic clearance particles in hypertriglyceridemic states. The present study investigates effect TG on HDL-associated apo A-I humans. was isolated plasma six normolipidemic men (mean age: 29.7 ± 2.7 years) fasting state after a five-hour intravenous infusion synthetic emulsion, Intralipid. Intralipid...
The metabolic syndrome is characterized by insulin resistance and abnormal apolipoprotein AI (apoAI) B-100 (apoB) metabolism that may collectively accelerate atherosclerosis. effects of atorvastatin (40 mg/day) micronised fenofibrate (200 on the kinetics apoAI apoB were investigated in a controlled cross-over trial 11 dyslipidemic men with syndrome. ApoAI studied following intravenous d(3)-leucine administration using gas-chromatography mass spectrometry data analyzed compartmental modeling....
To elucidate the molecular mechanisms underlying non-alcoholic fatty liver disease (NAFLD), we recruited 86 subjects with varying degrees of hepatic steatosis (HS). We obtained experimental data on lipoprotein fluxes and used these individual measurements as personalized constraints a hepatocyte genome-scale metabolic model to investigate differences in liver, taking into account its interactions other tissues. Our systems level analysis predicted an altered demand for NAD+ glutathione (GSH)...
OBJECTIVE Nonalcoholic fatty liver disease is highly prevalent in obese and type 2 diabetic individuals strongly associated with dyslipidemia inflammation. Weight loss and/or pharmacotherapy are commonly used to correct these abnormalities. RESEARCH DESIGN AND METHODS We performed a 16-week intervention trial of hypocaloric, low-fat diet plus 10 mg/day ezetimibe (n = 15) versus alone 10) on intrahepatic triglyceride (IHTG) content, plasma high sensitivity–C-reactive protein (hs-CRP),...
Extended-release niacin effectively lowers plasma TG levels and raises high-density lipoprotein (HDL) cholesterol levels, but the mechanisms responsible for these effects are unclear.We examined of extended-release (2 g/d) plus lovastatin (40 mg/d), relative to placebo, on kinetics apolipoprotein (apo) A-I apoA-II in HDL, apoB-100 TG-rich lipoproteins (TRL), intermediate-density (IDL) low-density (LDL), apoB-48 TRL 5 men with combined hyperlipidemia. Niacin significantly increased HDL apoA-I...
Lipoprotein(a) [Lp(a)], a low-density lipoprotein (LDL) particle covalently bound to apolipoprotein(a) [apo(a)], is potentially potent heritable risk factor for cardiovascular disease. We investigated the mechanism whereby evolocumab, monoclonal antibody against proprotein convertase subtilisin-kexin type 9 (PCSK9), lowers Lp(a). studied kinetics of Lp(a) particles in 63 healthy men, with plasma apo(a) concentration >5 nmol/L, participating an 8-week factorial trial effects evolocumab (420...
Monoclonal antibodies against proprotein convertase subtilisin kexin type 9 (PCSK9), such as evolocumab, lower plasma low-density lipoprotein (LDL)-cholesterol concentrations. Evolocumab is under investigation for its effects on cardiovascular outcomes in statin-treated, high-risk patients. The mechanism of action PCSK9 monoclonal metabolism remains to be fully evaluated. Stable isotope tracer kinetics can effectively elucidate the mode new lipid-regulating pharmacotherapies.We conducted a...
Hepatic accumulation of lipid substrates perturbs apolipoproteinB-100 (apoB) metabolism in insulin-resistant, obese subjects and may account for increased risk cardiovascular disease. In a placebo-controlled trial, we examined the independent combined effects decreasing cholesterol synthesis with atorvastatin (40 mg/day) triglyceride fish oils (4 g/day) on apoB kinetics. The were 48 viscerally obese, insulin-resistant men dyslipidemia who studied fasted state. We found that significantly...
Chronic low-grade inflammation may contribute to the increased risk of atherosclerosis in visceral obesity. Statin and fish oil have been reported antiinflammatory effects. We studied whether dyslipidemic, obese individuals plasma high-sensitivity C-reactive protein (hs-CRP) concentrations treatment with atorvastatin lowered hs-CRP concentrations.We compared hs-CRP, interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha) 48 10 lean normolipidemic men. The were then randomized (40...
The use of amino acids labeled with stable isotopes represents a relatively new approach for determining kinetic parameters apolipoprotein metabolism; thus, several aspects experimental protocols need to be defined.The aims the present study were determine whether a) different acid tracers or b) methods tracer administration affected apolipopotein (apo) B obtained by multicompartmental modeling, and c) compare very low density lipoprotein (VLDL)-apoB metabolic determined modeling those...
Aim: In this study, we investigated the association of plasma adiponectin and resistin concentrations with adipose tissue compartments in 41 free‐living men a wide range body mass index (22–35 kg/m 2 ). Methods: Using enzyme immunoassays, were measured. Intraperitoneal, retroperitoneal, subcutaneous abdominal posterior masses (IPATM, RPATM, SAATM PSAATM, respectively) determined using magnetic resonance imaging. Total (TATM) was measured bioelectrical impedance. Insulin resistance estimated...
To determine mechanisms contributing to decreased high-density lipoprotein cholesterol (HDL-C) and increased low-density (LDL-C) concentrations associated with hydrogenated fat intake, kinetic studies of apoA-I, apoB-100, apoB-48 were conducted using stable isotopes.Eight postmenopausal hypercholesterolemic women provided in random order 3 diets for 5-week periods. Two-thirds the was soybean oil (unsaturated fat), stick margarine (hydrogenated or butter (saturated fat). Total LDL-C levels...
The M\"ossbauer absorption spectra of $^{57}\mathrm{Fe}$ have been measured in the rare-gas matrices argon, krypton, and xenon with iron atomic concentrations from 0.3 to 3% matrix temperatures between 1.45 20.5 K. All show an line isomer shift $\ensuremath{\delta}=\ensuremath{-}0.75\ifmmode\pm\else\textpm\fi{}0.03$ mm/sec respect foil at 300 This is independent matrix, concentration, temperature. ascribed isolated atom (monomer) configuration $3{d}^{6}4{s}^{2}$. gives a new calibration...
The combination of ezetimibe, an inhibitor Niemann-Pick C1-like 1 protein (NPC1L1), and HMG-CoA reductase decreases cholesterol absorption synthesis. In clinical trials, ezetimibe plus simvastatin produces greater LDL-cholesterol reductions than does monotherapy. molecular mechanism for this enhanced efficacy has not been defined. Apolipoprotein B-100 (apoB-100) kinetics were determined in miniature pigs treated with (0.1 mg/kg/day), (10 or placebo (n = 7/group). Ezetimibe decreased (-79%)...
Isomeric chemical shifts were observed in the Mössbauer spectra of Au197 atoms dilute solutions 19 metals and semiconductors at 4.2°K. The interpreted as arising from transfer (conduction) electrons 6s shell gold, probably toward gold atom, alloys. A correlation was found between host electronegativities. relationship electron nickel d bands gold—nickel alloys is discussed. It shown that ground first excited nuclear states have radial charge moments sufficiently different (the state...
Statins are effective cholesterol-lowering agents that reduce cardiovascular disease risk but also have pleiotropic effects may extend to other lipid classes.The purpose of this article was investigate, in a post hoc analysis, the dose-dependent rosuvastatin on plasma sphingolipids and phospholipids men with metabolic syndrome.Subjects (n = 12) were studied randomized, double-blind, triple-crossover trial 5-week treatment period placebo or (10 40 mg/day) 2-week washouts between treatments....