This paper describes the immobilization of ten proteins and two low-molecular-weight ligands on mixed self-assembled monolayers (SAMs) alkanethiolates gold generated from tri(ethylene glycol)-terminated thiol 1 (HS(CH2)11(OCH2CH2)3OH) (chi(1) = 1.0-0.0) longer, carboxylic acid-terminated thiol2(HS(CH2)11(OCH2-CH2)6OCH2CO2H) (chi(2) 0.0-1.0). The was achieved by a two-step procedure: generation reactive N-hydroxysuccinimidyl esters acid groups 2 in SAM coupling these with amines protein or...

Tris(vancomycin carboxamide) binds a trivalent ligand derived from d -Ala- -Ala with very high affinity: dissociation constant ( K ) ≈ 4 × 10 –17 ± 1 M. High-affinity binding and monovalent are fundamentally different. In (and more generally, polyvalent) binding, occurs in stages, its rate can be accelerated by at sufficiently concentrations. is determined solely the for cannot added monomer. Calorimetric measurements system indicate an approximately additive gain enthalpy relative to...

This report describes a method for patterning ligands onto mixed SAMs of alkanethiolates on gold by microcontact printing (μCP). The were made from thiols presenting terminal tri(ethylene glycol) groups (HS(CH2)11(OCH2CH2)3OH, 1) and hexa(ethylene glycol)−CH2CO2H (HS(CH2)11(OCH2CH2)6OCH2CO2H, 2). Ligands printed using two-step procedure. carboxylic acid 2 first converted to reactive pentafluorophenyl esters. A freshly oxidized PDMS stamp, inked with ligand derivatized primary amine, was then...

This paper describes the fabrication of microarrays consisting G protein-coupled receptors (GPCRs) on surfaces coated with gamma-aminopropylsilane (GAPS). Microspots model membranes GAPS-coated were observed to have several desired properties-high mechanical stability, long range lateral fluidity, and a thickness corresponding lipid bilayer in bulk microspot. GPCR arrays obtained by printing membrane preparations containing GPCRs using quill-pin printer. To demonstrate specific binding...

The development of ultraminiaturized identification tags has applications in fields ranging from advanced biotechnology to security. This paper describes micrometer-sized glass barcodes containing a pattern different fluorescent materials that are easily identified by using UV lamp and an optical microscope. A model DNA hybridization assay these "microbarcodes" is described. Rare earth-doped glasses were chosen because their narrow emission bands, high quantum efficiencies, noninterference...

Abstract Hospital acquired infections (HAIs) and the emergence of antibiotic resistant strains are major threats to human health. Copper is well known for its high antimicrobial efficacy, including ability kill superbugs notorious ESKAPE group pathogens. We sought a material that maintains efficacy copper while minimizing downsides – cost, appearance metallic properties limit application. Here we describe copper-glass ceramic powder as an additive surfaces; mechanism based on controlled...

The application of resonance light scattering (RLS) particles for high-sensitivity detection DNA hybridization on cDNA microarrays is demonstrated. Arrays composed ∼2000 human genes ("targets") were hybridized with colabeled (Cy3 and biotin) lung probes at concentrations ranging from 8.3 ng/μL to 16.7 pg/μL. After hybridization, the arrays imaged using a fluorescence scanner. then treated 80-nm-diameter gold RLS Particles coated anti-biotin antibodies in white light, CCD-based imaging...

This work describes the binding of carbonic anhydrase (CA) to mixed self-assembled monolayers (SAMs) presenting benzenesulfonamide ligands at a surface consisting primarily tri(ethylene glycol) [(EG)3OH] groups. Surface plasmon resonance (SPR) quantified CA Two factors influenced CA: (a) density groups surface, and (b) coverage with molecules adsorbed these At low mole-fractions in SAM where is highly (>90%) reversible, we observe: an approximately 10-fold decrease observed bimolecular rate...

A trivalent derivative of vancomycin, tris(vancomycin carboxamide), [C6H3-1,3,5-(CONHC6H4-4-CH2NHCOV)3 (RtV3; V = vancomycin)], binds an analogous d-Ala-d-Ala, R'tL'3, (C6H3-1,3,5-[CONεH(Nα-Ac)-l-Lys-d-Ala-d-Ala]3) in water with a dissociation constant that is approximately 4 × 10-17 M, as estimated by HPLC using competitive assay against Nα,ε-diacetyl-l-Lys-d-Ala-d-Ala (L). This binding one the tightest known for low molecular weight organic species. The RtV3·R'tL'3 presence excess L could...

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTPorphyrin Amphiphiles as Templates for the Nucleation of Calcium CarbonateJoydeep Lahiri, Guofeng Xu, Daniel M. Dabbs, Nan Yao, Ilhan A. Aksay, and John T. GrovesView Author Information Departments Chemistry Chemical Engineering Princeton Materials Institute University, Princeton, New Jersey 08544 Cite this: J. Am. Chem. Soc. 1997, 119, 23, 5449–5450Publication Date (Web):June 11, 1997Publication History Received6 May 1996Published online11...

The use of weak, intermolecular forces to orchestrate the contruction multicomponent systems in membranes has significant implications diverse areas chemistry, biology, and medicine. We describe here construction characterization multi-heme molecular ensembles phospholipid vesicles. A trianionic zinc porphyrin was designed bind cytochrome c at membrane surface, while being anchored a spanning manganese interior via terminal imidazole. structure construct probed by fluorescence UV...

The paper describes the synthesis of surfaces based on self-assembled monolayers (SAMs) alkanethiolates gold that were designed to support adsorption bilayer lipid membranes, and feasibility using these for making high-density arrays (HDAs) membranes. involved (i) formation SAMs 16-mercaptohexadecanoic acid (MHA) gold; (ii) activation carboxylic groups interchain anhydrides; (iii) treatment reactive with Brij-76 (C18H37(OCH2CH2)n-10OH) (1) or Brij-76-amine (2) (C18H37(OCH2CH2)n-10NH2); (iv)...

This paper describes a method for the detection of single-base mismatches using DNA microarrays in format that does not require labeling sample ("target") DNA. The is based on disrupting fluorescence energy transfer (FRET) between fluorophore attached to an immobilized strand ("probe") and quencher-containing sequence complementary except artificial mismatch (e.g. 5-nitroindole, 3-nitropyrole, or abasic site) at site interrogation. As displacement FRET acceptor hybridization unlabeled probe...

Membrane-bound proteins represent the single most important class of drug targets. Arraying these is difficult because they typically need to be embedded in membranes maintain their correctly folded conformations. We describe here fabrication microarrays consisting G-protein-coupled receptors (GPCRs)--the largest family membrane-bound proteins-by robotic pin-printing on slides, and demonstrate assays for screening ligands arrays.

Microarrays of lipids containing gangliosides were fabricated on surfaces coated with γ-aminopropylsilane. A fluorescence microarray scanner was used to detect the binding toxins these arrays. The specific cholera and tetanus microspots GM1 GT1b gangliosides, respectively, demonstrated. These results suggest possibility using ganglioside microarrays for multiplexed toxin detection screening compounds that can inhibit binding.

This paper describes G-protein-coupled receptor (GPCR) microarrays on porous glass substrates and functional assays based the binding of a europium-labeled GTP analogue. The slides were made by casting frit impermeable then coating with gamma-aminopropyl silane (GAPS). emitted fluorescence was captured an imager time-gated intensified CCD detector. Microarrays neurotensin 1, cholinergic muscarinic 2, opioid mu, cannabinoid 1 fabricated pin printing. selective agonism each receptors observed....

Abstract Current technologies for studying ion channels are fundamentally limited because of their inability to functionally link channel activity cellular pathways. Herein, we report the use label-free cell phenotypic profiling decode composition and signaling an endogenous ATP-sensitive potassium (K ATP ) in HepG2C3A, a hepatocellular carcinoma line. Label-free agonist showed that pinacidil triggered characteristically similar dynamic mass redistribution (DMR) signals A431, A549, HT29 but...

Cytosolic phospholipase A2 (cPLA2) hydrolyzes the sn-2 ester of phospholipids and is believed to be responsible for receptor-regulated release arachidonic acid from phospholipid pools. The enzyme was assayed using vesicles containing arachidonate-containing substrate, such as 1-palmitoyl-2-arachidonoylphosphatidylcholine (PAPC) or 1-stearoyl-2-arachidonoylphosphatidylinositol (SAPI), dispersed within 1,2-dimyristoylphosphatidylmethanol (DMPM). We report here that shows an apparent...

Conventional assay methods for discovering and profiling drug-target interactions are typically developed on a target-by-target basis hence can be cumbersome to enable orchestrate. Herein the authors report solid-state ligand-binding that operates in multiplexed mode compound activity against micorarray-configured panel of G-protein-coupled receptor (GPCR) targets. The pharmacological fidelity system is high, its miniaturized "plug-and-play" format provides improved efficiency both terms...

Paint is a versatile material that can be used to coat surfaces for which routine disinfection practices may lacking. EPA-registered copper-containing supplemental residual antimicrobial paints could reduce the bioburden on often-neglected surfaces. An interventional study was conducted by painting walls of preschool restroom and metal locker in two hospital rooms with paint evaluate potential reduction compared non-copper-containing control paint. The reduced 57% lockers one room 63% paint;...

This work reports aqueous extractions from a copper-glass ceramic powder to make novel antimicrobial (AM) Cu( i )-containing additive for colorless water-based coatings. The in three coating types, gave high AM efficacy and low color impact.

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDirected Multi-Heme Self-Assembly and Electron Transfer in a Model MembraneJohn T. Groves, Gwendolyn D. Fate, Joydeep LahiriCite this: J. Am. Chem. Soc. 1994, 116, 12, 5477–5478Publication Date (Print):June 1, 1994Publication History Published online1 May 2002Published inissue 1 June 1994https://pubs.acs.org/doi/10.1021/ja00091a065https://doi.org/10.1021/ja00091a065research-articleACS PublicationsRequest reuse permissionsArticle...