Fibroblast growth factor 23 (FGF23) secreted by osteocytes is a circulating essential for phosphate homeostasis. High plasma FGF23 levels are associated with cardiovascular complications and mortality. Increases of in uremia antedate high phosphate, suggesting disrupted feedback regulatory loop or an extra-skeletal source this phosphatonin. Since induction expression injured organs has been reported we decided to examine the regulation gene protein expressions kidney whether kidney-derived...

ABSTRACT An association between lower bone mineral density (BMD) and presence of vascular calcification (VC) has been reported in several studies. Chronic kidney disease (CKD) causes detrimental disturbances the balance, turnover, development severe VC. Our group previously demonstrated expression Wnt inhibitors calcified arteries CKD rats. Therefore, we hypothesized that CKD-induced VC via this pathway signals to induces loss. To address novel hypothesis, developed a new animal model using...

Acute kidney injury (AKI) is associated with a very high mortality and an increased risk for progression to chronic disease (CKD). Ischemia-reperfusion (IRI) model AKI, which results in tubular damage, dysfunction of the mitochondria autophagy, decreased cellular nicotinamide adenine dinucleotide (NAD+) progressing fibrosis resulting CKD. NAD+ co-enzyme several proteins, including dependent sirtuins. augmentation, e.g. by use its precursor riboside (NR), improves mitochondrial homeostasis...

The calcium and phosphate homeostasis is regulated by a complex interplay between parathyroid hormone (PTH), fibroblast growth factor 23 (FGF23), calcitriol. Experimental studies have demonstrated an inhibitory effect of FG23 on PTH production secretion; the physiological role this regulation however not well understood. Surprisingly, in uremia, concomitantly elevated FGF23 levels are observed. gland rapidly loses its responsiveness to extracellular vitro functional cell line has currently...

In a new paradigm of etiology related to chronic kidney disease-mineral and bone disorder (CKD-MBD), injury may cause induction factors in the injured that are released into circulation thereby initiate maintain renal fibrosis CKD-MBD. Klotho is believed ameliorate CKD-MBD, while activin A might have detrimental effects. The unilateral ureter obstruction (UUO) model used here examine this concept by investigating early changes obstructed kidney, untouched contralateral vasculature which be...

Activin A is a new fascinating player in chronic kidney disease-mineral and bone disorder (CKD-MBD), which implicated progressive renal disease, vascular calcification, osteodystrophy. Plasma activin rises early the progression of disease. Disruption circadian rhythms related to increased risk several diseases are observed mineral homeostasis, parameters, plasma levels phosphate PTH. Therefore, we examined rhythm CKD-MBD-related parameters (phosphate, PTH, FGF23, klotho) healthy controls CKD...

Circadian rhythms in metabolism, hormone secretion, cell cycle and locomotor activity are regulated by a molecular circadian clock with the master suprachiasmatic nucleus of central nervous system. However, an internal is also expressed several peripheral tissues. Although about 10% all genes machinery parathyroid glands has not previously been investigated. Parathyroid secretion exhibits diurnal variation gene promoter contains E-box like element, known target proteins. Therefore, we...

Understanding the regulation of mineral homeostasis and function skeleton as buffer for Calcium Phosphate has regained new interest with introduction syndrome "Chronic Kidney Disease-Mineral Bone Disorder"(CKD-MBD). The very rapid minute-to-minute plasma-Ca(2+) (p-Ca(2+)) takes place via an exchange mechanism Ca(2+) between plasma bone. A labile Ca storage pool exists on bone surfaces storing excess or supplying when blood is lowered. Aim was to examine p-Ca(2+) in early phase acute uremia,...

Hyperphosphatemia and vascular calcification are frequent complications of chronic renal failure bone morphogenetic protein 7 (BMP7) has been shown to protect against development in uremia. The present investigation examined the potential reversibility established uremic by treatment rats with BMP7. A control model isogenic transplantation a calcified aorta from into healthy littermates whether normalization environment reversed calcification. Uremia were induced 5/6 nephrectomy, high...

The molecular circadian clock is an evolutionary adaptation to anticipate recurring changes in the environment and coordinate variations activity, metabolism hormone secretion. Parathyroid hyperplasia uremia a significant clinical challenge. Here, we examined transcriptome of murine parathyroid gland over 24 hours found rhythmic expression signature genes, such as Casr, Vdr, Fgfr1 Gcm2. Overall, 1455 genes corresponding 6.9% all expressed had rhythmicity. Biological pathway analysis...

Plasma (p-)activin A is elevated in chronic kidney disease-mineral and bone disorder (CKD-MBD). Activin inhibition ameliorates CKD-MBD complications (vascular calcification disease) rodent CKD models. We examined whether p-activin was associated with major adverse cardiovascular events (MACE), all-cause mortality patients.The study included 916 participants (741 patients 175 controls) from the prospective Copenhagen cohort. Comparisons of estimated glomerular filtration rate (eGFR), coronary...

Uremic vascular calcification is a regulated cell-mediated process wherein cells in the arterial wall transdifferentiate to actively calcifying resulting resembling bone formation. Wnt signalling established as major driver for vessel formation and maturation embryonic formation, disturbed might play role calcification. ICG-001 small molecule inhibitor that specifically targets coactivator CREB binding protein (CBP)/β-catenin-mediated signalling. In present investigation we examined effect...

Vascular calcification and bone disorder progress simultaneously in chronic kidney disease (CKD). Still, how the complex pathological mechanisms are linked is only sparsely understood. Up to now, focus has been on disturbed metabolism developing vascular calcification. However, our group recently demonstrated that negative effects formation mineralization as shown of normal recipient rats transplanted with calcified aorta from CKD rats. In present vitro study, hypothesis a direct crosstalk...

Pseudoterranoviasis is a zoonotic disease caused by nematode larvae of species within the genus Pseudoterranova (seal worm, cod worm). Most infections are gastrointestinal, oesophageal or pharyngeal, but here we report nasal infection. A 33-year-old patient suffering from rhinitis for 1.5 years recovered worm larva nose. Diagnosis was performed morphological and molecular characterization, showing causative agent to be third-stage decipiens (sensu stricto). Various infection routes discussed.

Increasing levels of magnesium in blood are associated with reduced risk cardiovascular disease chronic kidney (CKD). Magnesium supplementation may reduce the progression vascular calcification CKD. The diurnal pattern and effect fasting on urine CKD is unknown, knowledge this influence management supplementation. We included ten patients stage four without diabetes mellitus healthy controls. Participants were admitted to our hospital ward for a 24-h study period. Blood samples collected...

Uremic patients develop hyperplasia of the parathyroid glands due to disturbances in mineral metabolism. The hyperplastic parathyroids are associated with significant expression hormone (PTH)-related peptide (PTHrP). PTHrP has been shown have an autocrine/paracrine function parathyroids, but it is still uncertain if a secretory product gland and thereby possess endocrine actions. In cells severe adenomatous secondary hyperparathyroidism PTH found be co-localized same granules. act through...

Patients with chronic kidney disease (CKD) have a high risk of bone fractures. A circadian rhythmicity in turnover and mineralization appears to be importance for health. In CKD disturbances the rhythm various functions has been demonstrated indeed mineral metabolism is disturbed. The aim present study was compare markers ten patients healthy controls. Bone (C-terminal telopeptide type I collagen, tartrate-resistant acid phosphatase 5b, N-terminal propeptide procollagen, alkaline...

The Naked Mole Rat (NMR), Heterocephalus glaber, provides an interesting model for studying biomarkers of longevity due to its long lifespan more than 30 years, almost ten times longer that mice and rats. α-Klotho (klotho) is aging-suppressor gene, overexpression klotho associated with extended in mice. Klotho predominantly expressed the kidney. expression profile NMR has not previously been reported. present investigation studied kidney Rattus Norvegicus (RN) demonstrated was at same level...

Abstract Background and Aims Several studies report an association between low BMD vascular calcification (VC) in the general population CKD patients. Development of VC is a cell regulated process where smooth muscle alters phenotype to bone-like secretory cell. Our group has previously demonstrated expression Wnt inhibitors so we ask if presence affects bone metabolism. Method A novel model isogenic aorta transplantation (ATx) was developed used study. Severe uremic calcifications were...