A5023641202- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Electrolyte and hormonal disorders
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Pancreatic function and diabetes
- Membrane-based Ion Separation Techniques
- Renal function and acid-base balance
- Plant Stress Responses and Tolerance
- Drug Transport and Resistance Mechanisms
- Cardiovascular Function and Risk Factors
- Muscle metabolism and nutrition
- Bipolar Disorder and Treatment
- Pulmonary Hypertension Research and Treatments
- Dialysis and Renal Disease Management
- Nitric Oxide and Endothelin Effects
- Coronary Interventions and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Cardiac Imaging and Diagnostics
- Renal and related cancers
- Antiplatelet Therapy and Cardiovascular Diseases
- Metabolism and Genetic Disorders
- Cerebrovascular and Carotid Artery Diseases
- Neonatal Health and Biochemistry
- TGF-β signaling in diseases
University of Glasgow
2009-2018
Centre for Inflammation Research
2018
University of Birmingham
2018
Erasmus MC
2016
Pulmonary Vascular Research Institute
2011
Duke University
2011
Gosford Hospital
2008
National Institutes of Health
1986-2002
Johns Hopkins University
2001
Surgical Specialties (Canada)
2001
Scatter factor (also known as hepatocyte growth factor) is a glycoprotein secreted by stromal cells that stimulates cell motility and proliferation. In vitro, scatter vascular endothelial migration, proliferation, organization into capillary-like tubes. Using two different in vivo assays, we showed physiologic quantities of purified native mouse recombinant human induce angiogenesis (the formation new blood vessels). The angiogenic activity was blocked specific anti-scatter antibodies....
Background —Despite limiting elastic recoil and late vascular remodeling after angioplasty, coronary stents remain vulnerable to restenosis, caused primarily by neointimal hyperplasia. Paclitaxel, a microtubule-stabilizing drug, has been shown inhibit smooth muscle cell migration proliferation contributing We tested whether paclitaxel-coated are effective at preventing in porcine model of restenosis. Methods Results —Palmaz-Schatz were dip-coated with paclitaxel (0, 0.2, 15, or 187 μg/stent)...
We have examined the interaction of NH4+ and Li+ with Na+-H+ exchanger in rabbit renal microvillus membrane vesicles. Alkalinization intravesicular space, monitored [14C]DMO uptake by flow dialysis, resulted from addition Cl- salts Na+, Li+, or to external medium. The 22Na+ rate, assayed a rapid filtration technique, was saturable respect Na+ concentration (KT 6 mM Na+) competitively inhibited (Ki 1.9 mM) 4.3 mM). Efflux vesicles preloaded stimulated but Li+. conclude that has affinity for...
Abstract Endothelial cells are known to bind laminin, and two peptides derived from the laminin A (CTFALRGDNP) B1(CDPGYIGSR) chains block capillary‐like tube formation on a laminin‐rich basement membrane matrix, Matrigel. In present study, we have used various in vitro vivo assays investigate angiogenic‐biologic effects of third active site chain, CSRARKQAASIKVAVSADR (designated PA22‐2) endothelial cells. The SIKVAV‐containing peptide was as YIGSR‐containing for cell attachment but less than...
We examined the effect of amiloride on Na+-H+ exchange in rabbit renal cortical microvillus membrane vesicles. Amiloride inhibited both uphill Na+ accumulation induced by imposition a transmembrane Hin+ greater than Hout+ gradient and H+ efflux Naout+ Nain+ gradient. The inhibitory influx was rapidly reversible fully competitive (Ki 3.0 X 10(-5) M amiloride, KT 6.3 mM Na+). In addition, from vesicles preloaded with Na+. However, diuretic did not inhibit such other Na+-coupled transport...
ABSTRACT We performed differential cDNA hybridization using RNA from endothelial cells cultured for 4 hours on either plastic or basement membrane matrix (Matrigel), and identified early genes induced during the morphological differentiation into capillarylike tubes. The mRNA one clone, thymosin β4, was increased 5-fold. Immunostaining localized β4 in vivo both growing mature vessels as well other tissues. Endothelial transfected with showed an rate of attachment spreading components,...
The glucocorticoid dexamethasone, but not the mineralocorticoid aldosterone, increased amiloride-sensitive Na+-H+ exchange activity in rat proximal tubule brush border vesicles. Na+ uptake, independent of amiloride, was affected. decreased gradient-dependent phosphate uptake. Uptake absence a gradient inhibited. Dexamethasone did affect D-glucose These findings are consistent with effects glucocorticoids stimulating acid secretion and causing phosphaturia man animals may identify locus...
Based upon saturability, kinetic analysis and specific inhibition, transport for the organic anion, p-aminohippurate was found in isolated luminal antiluminal membrane vesicles from dog kidney cortex. The anions two membranes differed one another on basis of parameters ((Km, Vmax Ki probenecid) counterflow phenomenon (only displayed capacity to accelerate exchange p-aminohippurate). Also described is cation N1 methylnicotinamide each membrane. N1-methylnicotinamide are distinguished by (Km,...
Amiloride-sensitive Na+ -H+ exchange activity in brush border membrane vesicles isolated from rat proximal tubule was increased metabolic acidosis. The enhancement of required an intact adrenal gland or glucocorticoid supplements. Ammonium and phosphate excretions were during acidosis these also largely dependent on Amiloride-insensitive uptake passive H+ permeability not altered by the status animal. These findings are consistent with glucocorticoids having important regulatory role kidney...
Na+-H+ exchange activity, i.e., amiloride-sensitive Na+ and H+ flux, in renal proximal tubule brush border (luminal) membrane vesicles was increased the hyperthyroid rat decreased hypothyroid rat, relative to euthyroid animal. A positive correlation found between activity serum concentrations of thyroxine (T4) triiodothyronine (T3). The thyroid status animal did not alter amiloride-insensitive uptake. rate passive pH gradient dissipation higher from rats compared animals, a result which...
Chronic metabolic acidosis increased the Na+-H+ exchange activity in isolated renal brush-border membrane vesicles. Treatment altered initial rate ofNa+ uptake by increasing V, (acidotic, 15.3 f 0.7 nmol of Na+*mg"*2 s-l; normal, 11.3 0.9 s-'), and not apparent affinity KNa+ 10.2 0.5 mM; normal 0.6 mM).Metabolic resulted proportional increase 1 mM Na+ at every intravesicular pH measured.A positive cooperative effect on was found with acidity vesicles from both acidotic rats.When data were...
Objective To determine whether the ratio of cerebrospinal fluid (CSF) immunoglobulin kappa to lambda light chains at time multiple sclerosis (MS) diagnosis predicts disease progression and this was intrinsic CSF plasmablasts. Methods peripheral blood were obtained from patients undergoing elective diagnostic lumbar puncture included clinically isolated syndrome (CIS) (n=43), relapsing remitting MS (RRMS; n=50), primary progressive (PPMS; n=20) other neurological controls, both inflammatory...
Pre-steady-state kinetic measurements of 22Na+ uptake by the amiloride-sensitive Na+-H+ exchanger in renal brush border membrane vesicles (BBMV) were performed at 0 degrees C to characterize intermediate reactions exchange cycle. At 1 mM Na+, initial time course Na+ was resolved into three separate components: (i) a lag phase, (ii) an exponential or "burst" and (iii) constant velocity steady-state phase. Pulse-chase experiments using partially loaded BBMV showed no evidence for back-flux,...
Administration of the synthetic glucocorticoid dexamethasone to adrenalectomized rats increased Na+/H+ exchange activity in isolated renal brush border membrane vesicles. Treatment altered initial rate Na+ uptake by increasing Vmax (19.90 +/- 2.17 vs. 27.32 1.50 nmol.mg protein-1.5 s-1) and not apparent affinity KNa+ (8.33 1.11 7.94 1.60 mM). Dexamethasone treatment resulted a proportional increase 1 mM at every intravesicular pH measured. When these data were analyzed Hill equation, it was...
Abstract The formation of capillary‐like network structures by cultured vascular endothelial cells on reconstituted basement membrane matrix, Matrigel, models cell differentiation, the final step angiogenesis (Kubota et al., 1988; Grant 1989). When derived from bovine aorta and brain capillaries were plated DNA synthesis was suppressed a rapidly formed in 8–12 h. With time, broke down, resulting dense cellular cords radiating multiple clusters 16–24 Finally, multicellular aggregates as...
Allosteric control of Na+/H+ exchange by intracellular protons ensures rapid and accurate regulation the pH. Although this allosteric effect was heretofore thought to occur almost instantaneously, we report here occurrence a slower secondary activation epithelial exchanger (NHE)-3 isoform. This slow mode developed over course minutes unique NHE3 closely related isoform NHE5, but not observed in NHE1 or NHE2. Activation due increased density exchangers at cell surface, nor it accompanied...