A5059203864- Advanced MRI Techniques and Applications
- Functional Brain Connectivity Studies
- Neural dynamics and brain function
- Platelet Disorders and Treatments
- Cell Adhesion Molecules Research
- Nitric Oxide and Endothelin Effects
- Atherosclerosis and Cardiovascular Diseases
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Blood Coagulation and Thrombosis Mechanisms
- Social Media and Politics
- Traumatic Brain Injury and Neurovascular Disturbances
- Sociology and Education Studies
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Erythrocyte Function and Pathophysiology
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Optical Imaging and Spectroscopy Techniques
- Antiplatelet Therapy and Cardiovascular Diseases
- Eicosanoids and Hypertension Pharmacology
- Hemoglobinopathies and Related Disorders
- Kruppel-like factors research
- Biochemical and Structural Characterization
- Muscle activation and electromyography studies
- Cellular Mechanics and Interactions
Versiti Blood Center of Wisconsin
2015-2024
Technische Universität Braunschweig
2015
Washington University in St. Louis
2011
Medical College of Wisconsin
1992-2010
Marquette University
1997
Rationale: A hallmark of chronic inflammatory disorders is persistence proinflammatory macrophages in diseased tissues. In atherosclerosis, this associated with dyslipidemia and oxidative stress, but mechanisms linking these phenomena to macrophage activation remain incompletely understood. Objective: To investigate dyslipidemia, through modulation immunometabolism explore therapeutic potential targeting specific metabolic pathways. Methods Results: Using a combination biochemical,...
Abstract Regional‐specific average time courses of spontaneous fluctuations in blood oxygen level dependent (BOLD) MRI contrast at 9.4T lightly anesthetized resting rat brain are formed, and correlation coefficients between course pairs interpreted as measures connectivity. A hierarchy regional pairwise (RPCCs) is observed, with the highest values found thalamus cortex, both intra‐ interhemisphere, lower cortex thalamus. Independent sensory networks distinguished by two methods: data driven,...
This study examined the effects of blocking formation 20-hydroxyeicosatetraenoic acid (20-HETE) on acute fall in cerebral blood flow after subarachnoid hemorrhage (SAH) rat. In vehicle-treated rats, regional (rCBF) measured with laser-Doppler flowmetry fell by 30% 10 min injection 0.3 ml arterial into cisterna magna, and it remained at this level for 2 h. Pretreatment inhibitors 20-HETE, 17-octadecynoic (17-ODYA; 1.5 nmol intrathecally) N-hydroxy- N′-(4-butyl-2-methylphenyl)formamidine...
The relationship between high-density lipoprotein and pulmonary function is unclear. To determine mechanistic relationships we investigated the effects of genetic deletion apolipoprotein A-I (apoA-I) on plasma lipids, paraoxonase (PON1), pro-inflammatory HDL (p-HDL), vasodilatation, airway hyperresponsiveness oxidative stress, inflammation. ApoA-I null (apoA-I−/−) mice had reduced total cholesterol but increased compared with C57BL/6J mice. Although PON1 protein was in apoA-I−/− mice,...
Kindlins play an important role in supporting integrin activation by cooperating with talin; however, the mechanistic details remain unclear. Here, we show that kindlins interacted directly paxillin and this interaction could support αIIbβ3 activation. An exposed loop N-terminal F0 subdomain of was involved mediating interaction. Disruption kindlin binding to structure-based mutations significantly impaired function Both talin were required for enhance Interestingly, a direct between head...
Experimental asthma increases eosinophil and collagen deposition in the lungs of sickle cell disease (SCD) mice to a greater extent than control mice. However, effects on inflammation airway physiology remain unclear. To determine pulmonary mechanics SCD mice, hematopoietic stem transplantation was used generate chimeric hemoglobin A induced by sensitizing with ovalbumin (OVA). Airway were assessed using forced oscillation techniques. Mouse examined histologically physiologically. Cytokine,...
Abstract It is well understood that the different regions of body have cortical representations in proportion to degree innervation. Our current understanding rat upper extremity has been enhanced using functional MRI (fMRI), but these studies are often limited forepaw. The purpose this study describe a new technique allows us refine sensory and motor cerebral cortex by surgically implanting electrodes on major nerves providing direct electrical nerve stimulation while acquiring fMRI images....
Abstract Recent work demonstrates that epidermal keratinocytes are critical for normal touch sensation. However, it is unknown if contribute to evoked pain and hypersensitivity following tissue injury. Here, we used inhibitory optogenetic chemogenetic techniques determine the extent which keratinocyte activity contributes severe neuropathic accompanies chemotherapeutic treatment. We found inhibition largely alleviates paclitaxel-induced mechanical hypersensitivity. Furthermore, paclitaxel...
Recent work demonstrates that epidermal keratinocytes are critical for normal touch sensation. However, it is unknown whether contribute to touch-evoked pain and hypersensitivity after tissue injury. Here, we used a mouse model of paclitaxel treatment determine the extent which keratinocyte activity contributes severe neuropathic accompanies chemotherapy. We found inhibition by either optogenetic or chemogenetic methods largely alleviated paclitaxel-induced mechanical across acute persistent...
There is increasing evidence that the redox activities of pulmonary endothelial surface may have important implications for function both lungs and blood. Because inherent complexity intact organs, it can be difficult to study these in situ. Given availability appropriate indicator probes, multiple-indicator dilution (MID) method one approach dealing with some aspects this complexity. Therefore, objectives present were 1) evaluate potential utility two thiazine indicators, methylene blue...
Abstract The tyrosine kinase JAK2 is a critical component of intracellular JAK/STAT cytokine signaling cascades that prevalent in hematopoietic cells, such as stem cells and megakaryocytes (MKs). Individuals expressing the somatic V617F mutation commonly develop myeloproliferative neoplasms (MPNs) associated with venous arterial thrombosis, leading cause mortality. role hemostasis remains unclear. We investigated platelet hemostatic function using Jak2fl/fl Pf4-Cre (Jak2Plt−/−) mice lacking...
The regions of the body have cortical and subcortical representation in proportion to their degree innervation. rat forepaw has been studied extensively recent years using functional magnetic resonance imaging (fMRI), typically by stimulation electrodes directly inserted into skin forepaw. Here we stimulate nerve surgically implanted electrodes. A major distinction is that mostly sensory, whereas direct reveals not only sensory system but also deep brain structures associated with motor...
Calcium is a very important regulator of many physiological processes in vascular tissues. Most endothelial and smooth muscle functions highly depend on changes intracellular calcium ([Ca2+]i) nitric oxide (NO). In order to understand how [Ca2+]i, NO downstream molecules are handled by blood vessel response vasoconstrictors vasodilators, we developed novel technique that applies calcium-labeling (or NO-labeling) dyes with two photon microscopy measure handling production) isolated vessels....
An X-ray imaging technique designed to allow sequential diameter measurements of the cerebral vessels in intact, anesthetized small animals under relatively physiological conditions is described. The ferret and rabbit were chosen as potentially useful animal models for studying cerebrovascular system because advantageous anatomic characteristics these species. A commercially available inexpensive with a focal spot provides good spatial resolution. external carotid perfusion loop allows 1)...