A5101477238- Metabolism and Genetic Disorders
- Mitochondrial Function and Pathology
- Amino Acid Enzymes and Metabolism
- Nitric Oxide and Endothelin Effects
- Advances in Cucurbitaceae Research
- ATP Synthase and ATPases Research
- Biochemical and Molecular Research
- Insect Resistance and Genetics
- Heme Oxygenase-1 and Carbon Monoxide
- Insect and Pesticide Research
- Heat shock proteins research
- Antibiotic Resistance in Bacteria
- Cancer, Hypoxia, and Metabolism
- Plant Disease Resistance and Genetics
- Microtubule and mitosis dynamics
- Enzyme Structure and Function
- Cardiovascular Function and Risk Factors
- Protein Tyrosine Phosphatases
- Cardiac Ischemia and Reperfusion
- Plant biochemistry and biosynthesis
- Cardiac Arrest and Resuscitation
- Insect-Plant Interactions and Control
- Pharmaceutical and Antibiotic Environmental Impacts
- Fungal Biology and Applications
- Genetic Mapping and Diversity in Plants and Animals
Yunnan Agricultural University
2022-2024
Hunan Agricultural University
2023
Baoji University of Arts and Sciences
2022
BeiGene (China)
2021
China Agricultural University
2012-2021
National Institutes of Health
2019
National Heart Lung and Blood Institute
2019
Yueyang Hospital
2018
Shanghai University of Traditional Chinese Medicine
2018
Yangzhou University
2015-2016
The balance of nitric oxide (·NO) and superoxide anion (O⨪2) plays an important role in vascular biology. association heat shock protein 90 (Hsp90) with endothelial nitric-oxide synthase (eNOS) is a critical step the mechanisms by which eNOS generates ·NO. As capable generating both ·NO O⨪2, we hypothesized that Hsp90 might also mediate eNOS-dependent O⨪2 production. To test this hypothesis, bovine coronary cells (BCEC) were pretreated geldanamycin (GA, 10 μg/ml; 17.8 μm) then stimulated...
Acute lung injury (ALI) carries a high mortality in critically ill patients. Recent reports correlate elevated concentrations of endothelium-derived microparticles (EMPs) with diseases endothelial dysfunction. Many these have ALI sequelae. We hypothesize that EMPs contribute to cell (EC) dysfunction and development ALI. To test this hypothesis, we treated isolated vessels examined changes vasodilation. Endothelial cultures were incubated for stimulated nitric oxide (•NO) production synthase...
Low-density lipoprotein receptor-related protein 1 (LRP1) functions in endocytosis and intracellular signaling for a variety of structurally diverse ligands. Although LRP1 has been implicated several aspects neuronal function, molecular mechanisms underlying the activity remain unclear. Here, we describe pathway whereby transactivates Trk receptors. Binding tissue-type plasminogen activator or alpha(2)-macroglobulin (alpha(2)M) to resulted Src family kinase (SFK) activation SFK-dependent...
Abstract Spodoptera frugiperda (Lepidoptera: Noctuidae) is a widely distributed pest of corn. Since it invaded China in 2018, has caused serious damage to local corn production. Chlorantraniliprole, an anthranilic diamide insecticide, been used control lepidopteran pests. Tetrachloropyramid new allosteric modulator insecticide developed based on chlorantraniliprole, so similar mechanism and insecticidal effect. In this study, we investigated resistance levels chlorantraniliprole...
The relationship between high-density lipoprotein and pulmonary function is unclear. To determine mechanistic relationships we investigated the effects of genetic deletion apolipoprotein A-I (apoA-I) on plasma lipids, paraoxonase (PON1), pro-inflammatory HDL (p-HDL), vasodilatation, airway hyperresponsiveness oxidative stress, inflammation. ApoA-I null (apoA-I−/−) mice had reduced total cholesterol but increased compared with C57BL/6J mice. Although PON1 protein was in apoA-I−/− mice,...
The fall armyworm, Spodoptera frugiperda is one of the most destructive agricultural pests, which can complete their entire life cycle on various plants. At present, some detoxification genes have been proved to be involved in adaptability plants insects. However, genetics behind insect pest responses host switches, and ability adapt new plants, remain poorly understood. This study was conducted evaluate adaptation S. plant determine roles CYP321A9 CYP9A58 metabolism armyworm.The results...
Chronic hypoxia increases endothelial nitric oxide synthase (eNOS) production of (·NO) and cardioprotection in neonatal rabbit hearts. However, the mechanism by which this occurs remains unclear. Recent studies suggest that heat shock protein 90 (hsp90) alters eNOS function. In present study, we examined role hsp90 eNOS-dependent increased recovery postischemic left ventricular developed pressure (LVDP). Geldanamycin (GA), inhibits oxidative stress, decreased functional normoxic hypoxic To...
Objective: The relative contributions of the fraction inspired oxygen (FIO2) and atmospheric pressure (ATM) to cardioprotection are unknown. We determined whether product FIO2×ATM (oxygen partial pressure) controls extent hyperoxic+hyperbaric-induced involves activation nitric oxide synthase (NOS).
Pulmonary artery endothelial cells (PAEC) isolated from fetal lambs with in utero pulmonary hypertension (IPH) have phenotypical changes that lead to increased reactive oxygen species (ROS) formation and impaired angiogenesis. AMP-activated protein kinase (AMPK) is known be activated by ROS, which expected help angiogenesis IPH-PAEC. The objectives of this study were investigate AMPK responses IPH its role We observed that, compared control PAEC, IPH-PAEC decreased phosphorylation AMPKα...
Cucumber is an economically important vegetable crop worldwide. Powdery mildew (PM) one of the most severe diseases that can affect cucumber crops. There have been several research efforts to isolate PM resistance genes for breeding PM-resistant cucumber. In present study, we used a chromosome segment substitution line, SSL508-28, which carried from donor parent, JIN5-508, through twelve generations backcrossing with PM-susceptible inbred D8. We performed whole-genome resequencing SSL508-28...
In inflammatory diseases, tissue damage is critically associated with nitric oxide ((*)NO) and cytokines, which are overproduced in response to cellular release of endotoxins. Here we investigated the inhibitory effect roscovitine, a selective inhibitor cyclin-dependent kinases (CDKs) on (*)NO production mouse macrophages. RAW264.7 cells, found that roscovitine abolished induced by lipopolysaccharide (LPS). Moreover, significantly inhibited LPS-induced inducible synthase (iNOS) mRNA protein...
The emergence of NDM-1 containing multi-antibiotic resistant "Superbugs" necessitates the needs developing novel NDM-1inhibitors. In this study, we report discovery inhibitors by multi-step virtual screening. From a 2,800,000 drug-like compound library selected from ZINC database, generated focused inhibitor 298 compounds which 44 chemical were purchased and evaluated experimentally for their ability to inhibit in vitro. Three with micromolar IC50 values validated. most potent inhibitor,...
Cucurbitacin IIa is a triterpenoid isolated exclusively from Hemsleya plants, which non-steroidal anti-inflammatory drug that function as the main ingredient of prescription Hemslecin capsules and tablets in China. Synthetic biology provides new strategies for producing such valuable cucurbitacins large-scale, however, biosynthetic pathway cucurbitacin was still unknown heterologous production galactose medium expensive low-yield. In this study, functions two squalene epoxidases (HcSE1-2),...
Background We reported previously that Brown Norway ( BN ) rats are more resistant to myocardial ischemia/reperfusion (I/R) injury than Dahl S SS rats. To identify the unique genes differentially expressed in hearts of these rats, we used DNA microarray analysis and observed enoyl coenzyme A hydratase–containing domain 2 ECHDC 2) is highly (≈18‐fold) compared with hearts. Methods Results RT ‐ PCR , W estern blot, immunohistochemistry analyses verified was gene locates at chromosome 5 rat...
Objective GTP cyclohydrolase I (GCH1) is the rate-limiting enzyme for tetrahydrobiopterin biosynthesis and has been shown to be a promising therapeutic target in ischemic heart disease, hypertension, atherosclerosis diabetes. The endogenous GCH1-interacting partners have not identified. Here, we determined proteins rat. Methods Results A pulldown proteomics approach were used identify GCH1 interacting rat liver, brain, kidney. We demonstrated that interacts with at least 17 including...
Objective— The posttranslational regulation of GTP cyclohydrolase I (GCH-1), the rate-limiting enzyme for tetrahydrobiopterin (BH4) synthesis, remains elusive. Here, we identified specific phosphorylation sites on GCH-1 and characterized function these sites. Methods Results— Mass spectrometry studies showed overexpressed rat was phosphorylated at serine (S) 51, S167, threonine (T) 231 in HEK293 cells, whereas a computational analysis revealed 8 potential (S51, S72, T85, T91, T103, S130,...
Abstract The fall armyworm, Spodoptera frugiperda, is an invasive agricultural pest that a serious threat to production and global food security. Chemical control the most effective method for preventing outbreaks of S. frugiperda. However, insecticide resistance often develops as result prolonged pesticide use, molecular mechanisms involved in remain unclear. Insect cytochrome P450 monooxygenases play important role detoxification insecticides Lepidoptera. In our study, LC50 novel...