A5087955329- Hemoglobinopathies and Related Disorders
- Iron Metabolism and Disorders
- Asthma and respiratory diseases
- Complement system in diseases
- Child and Adolescent Health
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Respiratory and Cough-Related Research
- Emergency and Acute Care Studies
- Neonatal Respiratory Health Research
- Blood groups and transfusion
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Erythrocyte Function and Pathophysiology
- Child and Adolescent Psychosocial and Emotional Development
- Childhood Cancer Survivors' Quality of Life
- Inhalation and Respiratory Drug Delivery
- Pharmaceutical studies and practices
- Blood Coagulation and Thrombosis Mechanisms
- Neonatal Health and Biochemistry
- Autopsy Techniques and Outcomes
- Neuroscience of respiration and sleep
- Vitamin K Research Studies
- Pharmacological Effects and Toxicity Studies
- Adolescent and Pediatric Healthcare
- Healthcare Policy and Management
- Genetic Associations and Epidemiology
Washington University in St. Louis
2009-2018
St. Louis Children's Hospital
1999-2015
Anna Needs Neuroblastoma Answers
2014
Burroughs Wellcome Fund
2014
American Society of Hematology
2014
National Heart Lung and Blood Institute
2005-2014
Cancer Genomics Centre
2014
National Health Service
2014
Vanderbilt University
2014
Vanderbilt Health
2014
The serum complement system is a major mediator of inflammation reactions. Two the proteins, third (C3) and fifth (C5) components, are precursors potent phlogistic molecules, C3a C5a. C5a has chemotactic activity plays an active role in pulmonary inflammation. We present evidence suggesting that several including C5, synthesized locally lung alveolar type II epithelial cells. Lung tissue from normal mice secreted C5 protein similar to mouse serum, whereas C5-deficient did not. tissues both...
OBJECTIVE. Systemic corticosteroids are known to induce osteoporosis and increase the risk for fractures in adults children. Inhaled have been shown at risk; however, long-term prospective studies of children assess risks multiple short courses oral chronic inhaled not performed. Thus, we assessed effects on bone mineral accretion over a period years. METHODS. This was cohort follow-up study median 7 years who had mild-to-moderate asthma initially were randomly assigned into Childhood Asthma...
An analysis of a prospective cohort individuals with sickle cell anemia (SCA), enrolled from birth through adulthood, was conducted to determine if asthma is risk factor for death in SCA. All-cause mortality determined participants after adjusting known factors The study included 1,963 who were followed 18,495 patient-years. After controlling established factors, SCA and had more than two-fold higher (hazard ratio 2.36, 95% CI 1.21 4.62, p=0.01). To summarize,
Previous research has documented effects of both physical and social environmental exposures on childhood asthma. However, few studies have considered how these two environments might interact to affect asthma.This study aimed test interactions between chronic exposure traffic-related air pollution family stress in predicting biologic clinical outcomes children with asthma.Children asthma (n = 73, 9-18 years age) were interviewed about life stress, asthma-relevant inflammatory markers...
To ascertain the prevalence of and risk factors for obstructive sleep apnea syndrome (OSAS) in children with sickle cell anemia (SCA).Cross-sectional baseline data were analyzed from Sleep Asthma Cohort Study, a multicenter prospective study designed to evaluate contribution breathing abnormalities SCA-related morbidity ages 4 18 years, unselected OSAS symptoms or asthma. Multivariable logistic regression assessed relationships between status on basis overnight in-laboratory polysomnography...
Background Smoking while pregnant is associated with a myriad of negative health outcomes in the child. Some detrimental effects may be due to epigenetic modifications, although few studies have investigated this hypothesis detail. Objectives To characterize site-specific modifications conferred by prenatal smoking exposure within asthmatic children. Methods Using Illumina HumanMethylation27 microarrays, we estimated degree methylation at 27,578 distinct DNA sequences located primarily gene...
Rationale: Stress is associated with asthma morbidity in Puerto Ricans (PRs), who have reduced bronchodilator response (BDR).Objectives: To examine whether stress and/or a gene regulating anxiety (ADCYAP1R1) BDR PR and non-PR children asthma.Methods: This was cross-sectional study of (percent change FEV1 after BD) 234 PRs ages 9–14 years asthma. We assessed child using the Checklist Children’s Distress Symptoms, maternal Perceived Scale. Replication analyses were conducted two cohorts....
y-Interferon (IFN-y) is a well characterized lymphokine known to regulate many mononuclear phagocyte functions, including expression of class I and I1 major histocompatibility complex genes.The second component complement (C2) factor B are I11 gene products synthesized in phagocytes.Recombinant IFN-y increased the synthesis C2 primary cultures human phagocytes murine fibroblasts transfected with cosmid DNA bearing genes.In both cell types increases protein were detected at concentrations...
Genome-wide association studies of human gene expression promise to identify functional regulatory genetic variation that contributes phenotypic diversity. However, it is unclear how useful this approach will be for the identification disease-susceptibility variants. We generated profiles 22 184 mRNA transcripts using RNA derived from peripheral blood CD4+ lymphocytes, and genome-wide genotype data 516 512 autosomal markers in 200 subjects. screened cis-acting variants by testing mapping...
Studies of asthma admissions in the St. Louis metropolitan area have disclosed substantial numbers children with readmissions. To determine magnitude readmissions and attributes readmissions, a retrospective analysis 8,761 14,905 hospitalizations for January 1, 1990 through December 31, 1999 at two university affiliated children's hospitals was undertaken. Patient age, sex, race/ethnicity, residence, payor status, length stay, month admission were compared between patients admitted once...
Our objective was to determine if physician-diagnosed asthma increases the risk of acute chest syndrome (ACS) in children with sickle-cell disease (SCD) hospitalized for pain. study design a retrospective case-control all SCD patients, aged 2-21 years, pain during interval 1999-2000. Medical records first admissions were reviewed presence ACS admission. Cases defined as patients ACS, and controls without ACS. Independently, medical prior evidence asthma. Sixty-three cases 76 identified. No...
The third component of complement (C3) is a plasma glycoprotein with variety biologic functions in the initiation and maintenance host response to infectious agents. While hepatocyte primary source C3, mononuclear phagocytes contribute regulation tissue availability C3. Lipopolysaccharide (LPS), constituent cell walls gram-negative bacteria, consists polysaccharide moiety (core O antigen) covalently linked lipid portion (lipid A). Using metabolic labeling [35S]methionine,...
Fibroblast-like cells from synovial tissue obtained during arthroscopy in 4 young adults with recent knee trauma were biosynthetically labeled 35S-methionine, and protein production was quantitated by immunoprecipitation sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Synovial fibroblast-like synthesized C1r, C1s, C1 inhibitor, C2, C3, factor B, H, all the same sizes subunit structures as proteins skin fibroblasts. The capacity to synthesize these not lost passages or...