A5065323835- Muscle Physiology and Disorders
- Cholinesterase and Neurodegenerative Diseases
- Medicinal Plants and Neuroprotection
- Nicotinic Acetylcholine Receptors Study
- Sperm and Testicular Function
- Tissue Engineering and Regenerative Medicine
- Reproductive Biology and Fertility
- Animal testing and alternatives
- Tendon Structure and Treatment
- Exercise and Physiological Responses
- SARS-CoV-2 and COVID-19 Research
- Wildlife Ecology and Conservation
- Cardiomyopathy and Myosin Studies
- Laser Applications in Dentistry and Medicine
- Reproductive Physiology in Livestock
- Cardiovascular Effects of Exercise
- Viral Infections and Immunology Research
- Muscle metabolism and nutrition
- Memory and Neural Mechanisms
- Neuroendocrine regulation and behavior
- Rabbits: Nutrition, Reproduction, Health
- COVID-19 Clinical Research Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Estrogen and related hormone effects
- Nerve Injury and Rehabilitation
University of Ljubljana
2001-2025
BIA (Slovenia)
2013-2014
Ljubljana University Medical Centre
2013
Columbia University
2013
Canine cognitive dysfunction (CCD) is common in aged dogs and has many similarities with Alzheimer's disease. Unfortunately, like disease, CCD cannot be cured. In the present study, we treated our newly developed characterized butyrylcholinesterase inhibitor (BChEi). Seventeen were randomized into two groups (treated BChEi untreated) followed for 6 months at regular check-ups. The dogs' status was determined by a Dementia Scale (CADES) questionnaire tests. moderate impairment, treatment...
After three years of the SARS-CoV-2 pandemic, search and availability relatively low-cost benchtop therapeutics for people not at high risk a severe disease are still ongoing. Although vaccines new variants reduce death toll, long COVID-19 along with neurologic symptoms can develop persist even after mild initial infection. Reinfections, which further increase sequelae in multiple organ systems as well death, continue to require caution. The spike protein is an important target both...
<h3>Background</h3> Ultrasound-guided neuraxial anesthesia requires the application of ultrasound gel between transducer and skin to avoid signal drop-off. As needle is inserted, may be introduced intrathecally. The purpose this study was examine evidence an inflammatory response in subarachnoid space after intrathecal introduction. <h3>Methods</h3> Twelve piglets were sedated with azaperone 0.5 mg/kg intramuscularly anesthetized via face mask (2%–4% isoflurane 50% air-oxygen mixture). After...
Summary Testes samples of 52 brown hares ( Lepus europaeus L.), sacrificed between July and January, were subjected to immunohistochemical analysis. The terminal deoxynucleotidyl transferase‐mediated d'UTP nick end labelling (TUNEL) method was applied detect apoptosis; antibodies proliferating cell nuclear antigen (PCNA) used evaluate proliferation in the testes. In seminiferous epithelium, apoptotic processes evident from August early November with maximal values September. Cell death germ...
The aim of this study was to determine the pattern myosin heavy chain (MHC) isoform expressions within muscle fibres functionally diverse trunk and limb dog muscles using monoclonal antibodies that are specific MHC isoforms. We found three isoforms expressed in skeletal muscles. their determined existence 'pure' fibres, i.e. I IIa, both expressing only one isoform, 'hybrid' I/IIa IIa/x, co-expressed two While MHCI, MHCIIa MHCI/IIa corresponded myofibrillar ATPase type I, IIA IIC,...
To determine which myosin heavy chain (MHC) isoforms are expressed in canine skeletal muscles, different muscle samples of five mixed-breed dogs were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The separated MHC identified immunoblotting technique using a set specific monoclonal antibodies. compare the results electrophoretic and study, pattern isoform expression histochemical profiles fibres additionally demonstrated on serial sections...
To further elucidate the pattern of MHC isoform expression in skeletal muscles large mammals, this study brown bear, one largest mammalian predators with an extraordinary locomotor capacity, were analyzed. Fiber types longissimus dorsi, triceps brachii caput longum, and rectus femoris determined according to myofibrillar ATPase (mATPase) histochemistry expression, revealed by a set antibodies specific isoforms. The oxidative (SDH) glycolytic enzyme (alpha-GPDH) capacity fibers was...
BACKGROUND: Ultrasound gel nerve inflammation has been reported. We evaluated the extent and nature of after injection with endotoxin (positive), saline, or dry needle puncture (negative) controls peripheral blocks in piglets. METHODS: Selected nerves 12 piglets were localized by landmarks stimulator. Forty-eight hours injection, specimens examined for immunohistochemical cell differentiation/quantification cytokine expression using quantitative polymerase chain reaction. RESULTS: Both...
Abstract To gain a better understanding of the normal characteristics developing canine muscles, myosin heavy chain (MHC) isoform expression was analysed in axial and limb skeletal muscles 18 young dogs whose ages ranged from late prenatal stage to 6 months. We compared results immunohistochemistry using ten monoclonal antibodies, specific different MHC isoforms, enzyme‐histochemical reactions, which demonstrate activity myofibrillar ATPase, succinate dehydrogenase (SDH) α‐glycerophosphate...
The position and histological structure of the testes 33 brown hares (Lepus europaeus) were studied from July to December. From September, located in scrotum; October November, some animals, positioned more or less inguinal canal towards abdominal cavity, December none investigated animals had scrotum. Testes weighed a quantitative analysis tissue components was performed: diameter seminiferous tubules, depth epithelium, thickness tunica albuginea, peritubular relative proportion tubules...
Abstract Background: Canine cognitive dysfunction (CCD) is common in aged dogs and has many similarities with Alzheimer’s disease. Unfortunately, like disease, CCD cannot be cured. Methods: In the present study, we treated our newly developed characterized butyrylcholinesterase inhibitor (BChEi). Seventeen were randomized into two groups (treated BChEi untreated) followed for 6 months at regular check-ups. The dogs’ status was determined by a CADES questionnaire tests. Results: moderate...
Background: Canine cognitive dysfunction (CCD) is common in aged dogs and has many similarities with Alzheimer’s disease. Unfortunately, like disease, CCD cannot be cured. Methods: In the present study, we treated our newly developed characterized butyrylcholinesterase inhibitor (BChEi). Seventeen were randomized into two groups (treated BChEi untreated) followed for 6 months at regular check-ups. The dogs’ status was determined by a CADES questionnaire tests. Findings: moderate impairment,...
Bei einer Nervenblockade unter Ultraschallkontrolle wird im Allgemeinen ein Ultraschall-Kontaktgel verwendet, um den Hautwiderstand zu verringern. Dabei wurden inflammatorische Reaktionen der Nervengewebe beschrieben, wenn versehentlich geringe Mengen des Gels in das Lumen Blockadenadel gelangen und dann injiziert werden. Eine Gruppe aus Slowenien hat dieses Problem genauer untersucht.
In humans, estrogens are considered protective factor against atherosclerosis because the risk increases in postmenopausal women. However, it is not clear whether only factor, sex chromosomes also have an influence, and play same role all mammals. The mouse line C57BL/6J prone to develop atherosclerotic changes largest arteries after prolonged feeding of a high-fat diet containing cholesterol cholate (Paigen diet). We aimed examine effect hormones chromosome complement on development plaques...
Effective vaccines are needed to fight the COVID-19 pandemic. Forty golden hamsters were inoculated with two promising vaccine candidates and eighteen animals used in pilot trials viral challenge. ELISA assays performed determine endpoint serum titres for specific antibodies virus neutralisation tests evaluate efficacy of antibodies. All from vaccinated negative even after booster vaccinations changes vaccination protocol. We concluded that did not have sufficient neutralising properties....
Myosin heavy chain (MHC) isoform expression was established in the masseter, axial and limb skeletal muscles 18 young dogs from late prenatal to 6 months of age. Results obtained by immunohistochemical procedure using 11 monoclonal antibodies, SDS‐PAGE electrophoresis, Western‐blotting enzyme‐histochemical reactions were compared those found adult dogs. In both fetuses last stages development neonatal dogs, developmental isoforms MHC‐emb MHC‐neo prevalent. During first 10 days after birth,...