A5106174385- Thyroid Disorders and Treatments
- Growth Hormone and Insulin-like Growth Factors
- Sexual Differentiation and Disorders
- Blood groups and transfusion
- Cystic Fibrosis Research Advances
- Metabolism and Genetic Disorders
- Adrenal Hormones and Disorders
- Neonatal Health and Biochemistry
- Diet and metabolism studies
- Renal Diseases and Glomerulopathies
- Metabolism, Diabetes, and Cancer
- Ovarian function and disorders
- Pituitary Gland Disorders and Treatments
- Hormonal Regulation and Hypertension
- Biosimilars and Bioanalytical Methods
- Hormonal and reproductive studies
- Birth, Development, and Health
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Congenital heart defects research
- Cardiovascular Function and Risk Factors
- Thyroid Cancer Diagnosis and Treatment
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Vitamin C and Antioxidants Research
- Cancer Risks and Factors
- Obesity, Physical Activity, Diet
Manchester University NHS Foundation Trust
2018-2024
Royal Manchester Children's Hospital
2012-2022
The Christie Hospital
1993
University of Manchester
1986-1989
Manchester Royal Infirmary
1984-1986
Childhood obesity is increasingly associated with type 2 diabetes (T2D). Metformin reduces the risk for T2D in adult obese nondiabetic patients, but evidence children and young people inconclusive.The objective of study was to assess effect metformin on body mass index sd score (BMI-SDS), metabolic factors, adipokines.This a prospective, randomized, double-blind, placebo-controlled trial.The conducted at six pediatric endocrine centers United Kingdom.One hundred fifty-one hyperinsulinemia...
Abstract Ovarian function was assessed in 40 long term survivors who had received standard United Kingdom Acute Lymphoblastic Leukaemia (UKALL) protocols and were first clinical haematological remission. A menstrual pregnancy history taken (median age at assessment: 18.8 (12–34.7) years) the acquisition of adult secondary sexual characteristics confirmed each patient. Basal bloods for follicle stimulating hormone (FSH), luteinizing (LH), serum oestradiol estimations. Serum progesterone...
Active surveillance of primary congenital hypothyroidism (CH) in a multiethnic population with established newborn bloodspot screening. To estimate performance screening for CH at different test thresholds and calculate incidence CH. Prospective from June 2011 to 2012 3-year follow-up outcomes. Relative likelihood ratios (rLRs) estimated compare TSH 6 mU/L 8 mU/L, the nationally recommended standard 10 presumptive positive result. UK National Health Service. Clinician notification children...
Anti-Müllerian Hormone (AMH) concentration is high at birth in males, demonstrating the presence of functional testicular tissue prepubertal period, and acting as a useful marker investigation paediatric reproductive disorders. AMH also provides tool female virilization, premature ovarian failure polycystic syndrome childhood. Robust, assay-specific reference intervals are therefore required for clinical interpretation results. The aim this study was to derive age-specific males females aged...
Aim: Dried blood spots (DBS) are used for the analysis of more than 2000 biomarkers. We assessed a range analyte concentrations and diameters DBS. Materials & methods: DBS samples were created by application increasing volumes whole prepared UK NEQAS Quality Assurance Laboratory. Samples analyzed in four separate laboratories. Results: Volumes less 25 μl (8 mm) 75 (14 unsatisfactory analytical biases. Results obtained from peripheral subpunches tended to be higher those central subpunch....
<h3>Objective</h3> Congenital adrenal hyperplasia (CAH) is not currently included in the UK newborn screening programme. We investigated hypothesis that, owing to non-specificity of symptoms, a proportion males affected by salt-wasting (SW) CAH have died infancy without being diagnosed. <h3>Design</h3> Stored blood spot samples were analysed for 17α-hydroxyprogesterone (17-OHP) following groups: Infants born North West England, 1994 2006, who had 6 months age; (n=1198), neonatal reference...
Background Autoanalyser methods for the measurement of anti-Müllerian hormone have been introduced into clinical laboratories but few reports paediatric reference intervals using these new assays published. Methods After prior evaluation Roche Elecsys assay against Beckman Coulter modified second generation Hormone enzyme-linked immunosorbent samples from adult females, a cohort which had previously assessed Access was analysed assay. Results The measured significantly lower than In with...
Introduction UK screening for congenital hypothyroidism (CH) is based on dried blood spot Thyroid Stimulating Hormone (TSH). Scintigraphy may identify CH subtypes classified as dysplasia, gland in situ (GIS) and ectopia, but not performed all centres. We retrospectively investigated the role of scintigraphy to a single tertiary centre cohort. Methods Babies who screened positive between 2007 2017 were studied (n=418 534 783). outcomes correlated with TSH levothyroxine dose. GIS patients...
Children with salt-wasting adrenal insufficiency are managed glucocorticoid and mineralocorticoid replacement. Measurement of renin activity or concentration alongside blood electrolyte levels is used to monitor the adequacy Our unit changed from using carried out a service review assess whether this influenced decision-making for fludrocortisone dosing. In total, 50 measurements plasma were analysed on separate cohorts before after assay change, values standardised multiples upper limit...
There are limited published data on the performance of percentage haemoglobin A (Hb A) as a screening test for beta thalassaemia major in newborn period. This paper aims to analyse derived from national bloodspot programme sickle cell disease period.Newborn 2,288,008 babies were analysed. Data reported NHS Sickle Cell and Thalassaemia Screening Programme England period 2005 2012 also reviewed identify any missed cases (4,599,849 babies).Within cohort births, 170 identified screen positive...
Background 1,25-Dihydroxy vitamin D3 (DHVD) is the active metabolite of D, required to maintain blood calcium concentrations. Measurement has proved challenging as it circulates in picomolar concentrations and must be differentiated from other dihydroxyvitamin D species. Clinically, essential able determine cause hypercalcaemia, which may due DHVD excess. Methods The liquid chromatography-mass spectrometry (LCMS) assay been developed uses immunoextraction 0.5 mL serum followed by Amplifex™...
Background A subnormal cortisol response (30 min level (C 30min )<550 nmol/L) to synthetic adrenocorticotrophic hormone/Synacthen test (SDST) in all infants does not necessarily indicate underlying or persistent hypothalamic–pituitary–adrenal axis pathology. Methods We retrospectively evaluated the diagnoses and outcomes 68 who had a SDST at age <6 months from 2011 2014. Results 29 (43%) SDST. Causative pathology was identified 9/29 (31%). In 20/29 (69%) with no pathology, repeat...
Objective To determine the necessity for acid-washed containers 24-h urine copper analysis. Methods Copper solutions, with concentrations relevant to clinical decision limits, were prepared by spiking both assay diluent and unknown samples calibrator. Samples split between plain containers, analysis was performed using inductively coupled mass spectrometry (ICP-MS). Results Measurement of in spiked showed minimal concentration bias containers. Conclusions Acid-washed are not required...
A thyroid function test (TFT) is a common investigation, often undertaken by general paediatricians and practitioners. TFT involves the measurement of serum free thyroxine (fT4) stimulating hormone (TSH) to guide diagnosis management hypothyroidism hyperthyroidism, requiring treatment with medications such as levothyroxine (LT4) carbimazole. Thyroid hormones play crucial role in early life neurodevelopment; therefore, correct interpretation required optimise outcomes. needs be contextualised...
In 2015, U.K. newborn screening (NBS) laboratory guidelines were introduced to standardize dried blood spot (DBS) specimen quality acceptance and specify a minimum acceptable DBS diameter of ≥7 mm. The UK 'acceptable' avoidable repeat rate (AVRR) is ≤2%. To assess inter-laboratory variability in acceptance/rejection, two sets colored scanned images (
This nationwide prospective surveillance study aimed to estimate the performance of current UK recommended TSH threshold (10 mU/L on day 5 after birth) for newborn blood spot screening compared lower thresholds: 8 and 6 mU/L. Over a 12-month period, authors included all patients with positive based (n=629) or suspected congenital hypothyroidism clinical grounds (n=21). Further, three year follow-up period was added exclude not confirmed cases those who successfully discontinued levothyroxine...
<h3>Background</h3> Identification of Congenital Hypothyroidism (CHT) with Blood Spot (BS) Thyroid Stimulating Hormone (TSH) is an essential part Newborn Screening (NBS) in the UK. A screen positive result requires confirmation plasma free thyroxine (fT4) and TSH. Technetium-99m scintigraphy can be used to define size location thyroid. We investigated utility identify cause CHT impact on clinical management. <h3>Methods</h3> Newborns who were for (initial screening BS TSH >20 mU/L or...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)