Gelsemium elegans (Gardner and Champ.) Benth. (Gelsemiaceae) (GEB) is a toxic plant indigenous to Southeast Asia especially China, has long been used as Chinese folk medicine for the treatment of various types pain, including neuropathic pain (NPP). Nevertheless, limited data are available on understanding interactions between ingredients-targets-pathways. The present study integrated network pharmacology experimental evidence decipher molecular mechanisms GEB against NPP. candidate...

Carbapenem resistant Enterobacteriaceae (CRE) has become a serious public health problem. Limited information is available about the treatment options that physicians used to fight CRE infections and related clinical outcomes in China. The aim of present study was explore patients with bloodstream infection (BSI) Chinese teaching hospital. A retrospective conducted during 2011 2015 one Demographic, microbiological characteristics enrolled subjects were collected from medical records. Data...

Colistin has been considered as a last-line treatment option in severe infections caused by multidrug-resistant (MDR) gram-negative pathogens. However, the emergence of mobile colistin resistance gene (mcr-1) challenged this viewpoint. The aim study is to explore prevalence mcr-1 Escherichia coli (E. coli) Chinese teaching hospital, and investigate their molecular characteristics. A total 700 E. isolates were used screen PCR sequencing university hospital from August 2014 2015....

The emergence and prevalence of carbapenem-resistant Enterobacteriaceae (CRE) have drawn worldwide attention. Ceftazidime/avibactam (CAZ/AVI) gives us a valuable alternative strategy to treat CRE infections. Unfortunately, CAZ/AVI resistance could occur during treatment. CAZ/AVI-resistant Carbapenem-resistant Klebsiella pneumoniae (CR-KP) (KP137060) earlier CAZ/AVI-susceptible isolate (KP135194) from the same hospitalized patient were collected at Fujian Medical University Union Hospital...

Colorectal cancer (CRC) remains one of the leading causes cancer-related death worldwide. Gelsemium elegans Benth (GEB) is a traditional Chinese medicine commonly used for treatment gastrointestinal cancer, including CRC. However, underlying active ingredients and mechanism remain unknown. This study aims to explore components functional mechanisms GEB in treating CRC by network pharmacology-based approaches.Candidate compounds were collected from Traditional Medicine@Taiwan, Medicines...

The aim of this study was to characterize the carbapenemases in carbapenem-resistant Klebsiella pneumoniae (CR-KP) from a Chinese teaching hospital. A total 40 CR-KPs were screened for presence carbapenemases. Minimum inhibitory concentrations determined by agar dilution. modified Hodge test used detection carbapenemase production. Carbapenemase, extended-spectrum β-lactamase, and AmpC genes detected using polymerase chain reaction (PCR) sequencing. conjugation performed broth culture mating...

Automated systems (MicroScan WalkAway 96 Plus, Phoenix 100, and Vitek 2 Compact) are widely used in clinical laboratories nowadays. The aim of this study is to evaluate the performance these three for susceptibility testing aminoglycosides fluoroquinolones against Carbapenem-resistant Enterobacteriaceae (CRE). A total 75 CRE isolates were study. Quinolone resistance determinants (QRDs) (qnrA, qnrB, qnrC, qnrD, qnrS, aac(6′)-Ib-cr, oqxAB qepA) aminoglycoside (ARDs) (aac(6′)-Ib, armA, npmA,...

Antibiotic therapy can affect not only the pathogenic bacteria, but also commensal microorganisms in gut flora of humans, which might serve as a reservoir antimicrobial resistance genes. 1 Escherichia coli be found widely nature, and implicated human infectious diseases.4][5] In China, however, very little is known about prevalence antibiotic resistant genes intestinal tracts healthy people.The aim our study to investigate diversity integrons fecal E. strains recovered from persons China.In...

Background: Hospital wastewater (HWW) promotes the spread of carbapenem resistance genes (CRGs). Aeromonas carry a large number CRGs in HWW, they may play role as suitable reservoir for CRGs, while resistomes HWW are still poorly characterized regarding resistant . Thus, aim study was to evaluate molecular epidemiological characteristics HWW. Methods: A total 33 were isolated from Antimicrobial susceptibility testing and polymerase chain reaction (PCR) used assess antimicrobial profiles....

Escherichia coli (E. coli) ST1193 is an emerging fluoroquinolones-resistant and virulent lineage. Large gaps remain in our understanding of the evolutionary processes differences this Therefore, we used 76 E. genomes to detect strain-level genetic diversity phylogeny lineage globally. All possessed fimH64, filCH5, fumC14. There was 94.7% isolates classified as O-type O75. 9.33% that K5 capsular, while 90.67% K1 capsular. The core genome analysis revealed all were divided into two...

Sequence type 1193 is a new such lineage among fluoroquinolone-resistant Escherichia coli, which has risen dramatically within the last several years. However, reasons for rapid emergence and successful spread of E. coli ST1193 remain unclear. The aim this study was to compare pathogenicity survivability features with global epidemic lineage, ST131.A total 30 were used in study. Isolates divided into two groups, (n=15) ST131 (n=15). Adhesion invasion T24 cells resistance serum quantified...

Two series of novel N 6 derivatives 8-azapurine I and II were designed as antiplatelet agents.

A novel series of 4-aryl-5,7-dihydro-6H-pyrrolo[2,3-d]pyrimidin-6-one derivatives were designed as a phosphoinositide 3-kinase α (PI3Kα) inhibitor by scaffold hopping. The target compounds, characterized 1H-NMR, 13C-NMR and high resolution (HR)-MS, synthesized from diethyl malonate ethyl chloroacetate nucleophilic substitution, ring-closure, chlorination Suzuki reaction, etc. biological activities evaluated with cytotoxic activity in vitro on Uppsala 87 Malignant Glioma (U87MG) prostate...

O16:H41 is an important subclone among Escherichia coli (E. coli) sequence type (ST) 131, which has risen dramatically in recent years. However, reasons for the rapid increase of E. O16:H41-ST131 remain unclear. The aim this study was to compare pathogenicity and survivability features with global epidemic O25b:H30-ST131 lineage.Sixteen ST131 were divided into two groups: group (n=6) (n=10). Adhesion invasion activity different isolates measured using human T24 cells. Biofilm production...

To obtain new anticancer agents with antimetastatic adjunct efficacy, a series of novel N4 -hydrazone derivatives 5,7-dihydro-6H-pyrrolo[2,3-d]pyrimidin-6-one were designed and synthesized by an eight-step reaction, appropriate yields. All the compounds evaluated for their antiproliferative activity against A549 MCF-7 cells antiplatelet aggregation in vitro. The results showed that 25 35 not only potent (IC50 = 15.3 21.4 μM) 15.6 10.9 cell lines but also certain (inhibition rates: 47.0%...

Pharmacoeconomics is an emerging interdiscipline, and microeconomics the professional foundation of it. However at present teaching system in pharmacoeconomics course has not been established. In this article, starting from goal cultivating applied pharmaceutical talents, characteristics was analyzed combination with practice, new model proposed on basis Some exploration practice had carried three aspects, contents, methods effect evaluating, strategies put forward to improve quality...