A5066169002- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Allelopathy and phytotoxic interactions
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Plant responses to elevated CO2
- Plant responses to water stress
- Plant tissue culture and regeneration
- Weed Control and Herbicide Applications
- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- Peroxisome Proliferator-Activated Receptors
- Light effects on plants
- Plant Reproductive Biology
- Nitric Oxide and Endothelin Effects
- Pesticide and Herbicide Environmental Studies
- Plant Genetic and Mutation Studies
- Aluminum toxicity and tolerance in plants and animals
- Adipose Tissue and Metabolism
University of Zurich
2016-2024
Institute of Plant Biology
2023
Planta
2023
Consejo Superior de Investigaciones Científicas
2009-2021
Estación Experimental del Zaidín
2009-2014
Instituto de Biología Molecular y Celular de Plantas
2012
Centro de Investigaciones Biológicas Margarita Salas
2009
Abstract Cadmium (Cd) toxicity has been widely studied in different plant species; however, the mechanism involved its as well cell response against metal have not established. In this work, using pea (Pisum sativum) plants, we effect of Cd on antioxidants, reactive oxygen species (ROS), and nitric oxide (NO) metabolism leaves cellular, molecular, biochemical approaches. The growth plants with 50 μ m CdCl2 affected differentially expression superoxide dismutase (SOD) isozymes at both...
Starch serves functions that range over a timescale of minutes to years, according the cell type from which it is derived. In guard cells, starch rapidly mobilized by synergistic action β-AMYLASE1 (BAM1) and α-AMYLASE3 (AMY3) promote stomatal opening. leaves, typically accumulates gradually during day degraded at night BAM3 support heterotrophic metabolism. During osmotic stress, in light stress-activated BAM1 release sugar sugar-derived osmolytes. Here, we report AMY3 also involved...
Peroxisomes, single-membrane-bounded organelles with essentially oxidative metabolism, are key in plant responses to abiotic and biotic stresses. Recently, the presence of nitric oxide (NO) described peroxisomes opened possibility new cellular functions, as NO regulates diverse biological processes by directly modifying proteins. However, this mechanism has not yet been analysed peroxisomes. This study assessed S-nitrosylation pea-leaf peroxisomes, purified S-nitrosylated peroxisome proteins...
During photosynthesis of higher plants, absorbed light energy is converted into chemical that, in part, accumulated the form transitory starch within chloroplasts. In following night, mobilized to sustain heterotrophic metabolism plant. β-amylases are glucan hydrolases that cleave α-1,4-glycosidic bonds and release maltose units from non-reducing end polysaccharide chain. Arabidopsis, nocturnal degradation involves mainly β-amylase-3 (BAM3). A second β-amylase isoform, β-amylase-1 (BAM1),...
In this work the differential response of adult and young leaves from pea (Pisum sativum L.) plants to herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) (23 mm) applied by foliar spraying was investigated. The concentration 2,4-D time treatment (72 h) were previously optimized in order visualize its toxic effects on plants. Under these conditions, induced severe disturbances mesophyll cells structure proliferation vascular tissue increased acyl-CoA oxidase (ACX), xanthine (XOD) lipoxygenase...
2,4-Dichlorophenoxyacetic acid (2,4-D) is a synthetic auxin used as herbicide to control weeds in agriculture. A high concentration of 2,4-D promotes leaf epinasty and cell death. In this work, the molecular mechanisms involved toxicity are studied by analysing Arabidopsis plants accumulation reactive oxygen species (ROS) nitric oxide (NO), their effect on cytoskeleton structure peroxisome dynamics. (23mM) epinasty, whereas process was prevented EDTA, which can reduce ·OH accumulation. The...
The accumulation of starch within photosynthetic tissues and dedicated storage organs has been characterized extensively in many species, a function buffering carbon availability or fueling later growth phases, respectively, proposed. However, developmentally regulated turnover heterotrophic other than is poorly characterized, its not well understood. Here, we report on the characterization during flower, early embryo, silique development Arabidopsis (Arabidopsis thaliana) using combined...
Although structurally similar to the natural plant hormone indol-3- acetic acid, auxin herbicides were developed for purposes other than growth, and have been successfully used in agriculture last 60 years. Concerted efforts are being made understand decipher precise mechanism of action IAA synthetic auxins. Innumerable results need be interconnected resolve puzzle biology mode herbicides. To date, different breakthroughs providing more insights into process plant-herbicide interactions....
Plant protoplasts provide starting material for of inducing pluripotent cell masses that are competent tissue regeneration in vitro, analogous to animal induced stem cells (iPSCs). Dedifferentiation is associated with large-scale chromatin reorganisation and massive transcriptome reprogramming, characterised by stochastic gene expression. How this cellular variability reflects on organisation individual what factors influence transitions during culturing largely unknown. Here, we used...
Abstract The herbicide 2,4‐dichlorophenoxyacetic acid (2,4‐ D ) causes uncontrolled cell division and malformed growth in plants, giving rise to leaf epinasty stem curvature. In this study, mechanisms involved the regulation of induced by 2,4‐ were studied using different chemicals reactive oxygen species ( ROS accumulation (diphenyleniodonium, butylated hydroxyanisole, EDTA , allopurinol), calcium channels L a C l 3 ), protein phosphorylation (cantharidin, wortmannin) ethylene...
Abstract The synthetic auxin 2,4‐dichlorophenoxyacetic acid (2,4‐D) functions as an agronomic weed control herbicide. High concentrations of 2,4‐D induce plant growth defects, particularly leaf epinasty and stem curvature. Although the triggered reactive oxygen species (ROS) production, little is known about its signalling. In this study, by using a null mutant in peroxisomal acyl CoA oxidase 1 ( acx1‐2 ), we identified acyl‐coenzyme A (ACX1) one main sources ROS production and, part, also...
Abstract Plant protoplasts constitute the starting material to induce pluripotent cell masses in vitro competent for tissue regeneration. Dedifferentiation is associated with large-scale chromatin reorganisation and massive transcriptome reprogramming, characterized by stochastic gene expression. How this cellular variability reflects on organisation individual cells what are factors influencing transitions during culturing largely unknown. High-throughput imaging a custom, supervised image...