A5016838702- Probiotics and Fermented Foods
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolites in Food Biotechnology
- Enzyme Production and Characterization
- Gut microbiota and health
- Cleft Lip and Palate Research
- Glycosylation and Glycoproteins Research
- Proteins in Food Systems
- Digestive system and related health
- Biopolymer Synthesis and Applications
- Medical and Biological Sciences
- Enzyme Catalysis and Immobilization
- Biochemical effects in animals
- Infant Nutrition and Health
- Craniofacial Disorders and Treatments
- Orthodontics and Dentofacial Orthopedics
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Polysaccharides and Plant Cell Walls
- Carbohydrate Chemistry and Synthesis
- Dental Anxiety and Anesthesia Techniques
- Skin and Cellular Biology Research
- Advanced Glycation End Products research
- thermodynamics and calorimetric analyses
- Freezing and Crystallization Processes
Tohoku University
1977-2023
National Hospital Organization
2005
Morinaga (Japan)
1997
Niigata College of Technology
1983
We previously identified a gene, reg (i.e. regenerating gene), in the screening of islet-derived cDNA library rat (Terazono, K., Yamamoto, H., Takasawa, S., Shiga, Yonemura, Y., Tochino, and Okamoto, H. (1988) J. Biol. Chem. 263, 2111-2114), isolated human gene (Watanabe, T., Yonekura, Terazono, (1990) 265, 7432-7439); cDNAs encode 165- 166-amino acid proteins, respectively. Until now, it was thought that there is single locus for Reg protein mammalian genome. In this study, we two distinct...
Liquid droplet has emerged as a flexible intracellular compartment that modulates various cellular processes. Here, we uncover an antimetastatic mechanism governed by the liquid droplets formed through liquid–liquid phase separation (LLPS) of SQSTM1/p62 and neighbor BRCA1 gene 1 (NBR1). Some tyrosine kinase inhibitors (TKIs) initiated lysosomal stress response promotes LLPS p62 NBR1, resulting in spreading p62/NBR1 droplets. Interestingly, droplet, degradation RAS-related C3 botulinum toxin...
Lactobacillus acidophilus group and a Lact. reuteri isolated from human faeces were examined for production of antimicrobial agents against 16 strains six species food-borne enteric pathogenic bacteria. Several gasseri showed wide inhibitory activity the tested Gassericin A produced by LA39 was one most widely active bacteriocins. It bactericidal without causing cell lysis.
Lactobacillus reuteri LA 6, isolated from infant faeces, produced an antimicrobial agent active against acidophilus JCM 2125, delbrueckii subsp. bulgaricus 1002 and lactis 1148 1248. The was sensitive to proteolytic enzymes retained activity after heating at 100°C for 20 min. This a bacteriocin has been designated as reutericin 6. Reutericin 6 exceeds 200 kDa determined by ultrafiltration studies. Activity cells both bacteriocidal bacteriolytic.
Bacteriocin production can be easily detected by using microdilution wells. The method is very convenient for screening bacteria bactericidal activity.
A new method was developed for the determination of extracellular polysaccharides produced by microorganisms. Culture supernatants were ultrafiltered through a membrane having molecular exclusion limit 5000 Da. Mono‐ and disaccharides removed repeated washings with water then polysaccharide in retentate determined colorimetry. The has been successfully applied to determine production Lactobacillus delbrueckii subsp. bulgaricus NCFB 2483 milk.
We compared the biologic activities of 85-kDa macrophage-CSF (85-kDa M-CSF), which is fully active, and a proteoglycan M-CSF (PG-M-CSF). Both originate from same precursor, but latter retains carboxyl-terminal portion, must be proteolytically removed precursor to generate uniquely modified by chondroitin sulfate glycosaminoglycan chain. PG-M-CSF supported formation murine macrophage colonies such as M-CSF. Furthermore, stimulated proliferation bone marrow macrophages, an M-CSF-dependent cell...
The accessory polymer of the capsular polysaccharide a 'kefiran'‐producing Lactobacillus kefiranofaciens was studied. teichoic acid L. K1 extracted from cell with 5% trichloroacetic (w v) at 5°C for 24 h and purified by ion‐exchange chromatography on DEAE‐Toyopearl 650 M linear gradient (NH4)2CO3; yield only 2%. has molecular weight 22000 is composed D‐glucose, 2‐acetamide‐2‐deoxy‐D‐glucose, glycerol phosphorus in molar ratio 1.0 : 2.0 2.3 1.1. low suggested that kefiran main cell‐wall...
β‐Galactosidase (β‐gal) and 6‐phospho‐β‐galactosidase (P‐β‐gal) activities were measured in a total of 34 strains from Lactobacillus acidophilus, crispatus, amylovorus, gasseri johnsonii. The Lact. have P‐β‐gal but little or no β‐gal activities. other species only very
beta-N-Acetylhexosaminidase [EC 3.2.1.30] was purified 820-fold from the viscera of Halocynthia roretzi by Sephadex G-200 gel filtration and chromatography on columns DEAE-Sephadex CM-Sephadex. The final preparation sufficiently free alpha-N-acetylglucosaminidase, alpha-N-acetylgalactosaminidase, alpha- beta-glucosidases, beta-galactosidases, beta-mannosidases, alpha-L-fucosidase, gave one protein band disc electrophoresis. Two different molecular weight forms which depended upon pH were...
Journal Article Properties of β‐galactosidase Lactobacillus kefiranofaciens K‐1 isolated from kefir grains Get access K. Itoh, Itoh Laboratory Animal Products Chemistry, Faculty Agriculture, Tohoku University, 1‐1 Tsutsumidoriamamiyamachi, Aoba‐ku, Sendai 981 Search for other works by this author on: Oxford Academic Google Scholar T. Toba, Toba S. Adachi 981*Shoukei Women's Junior College, 4–10‐1 Yurigaoka, Natori 981‐12, Japan Letters in Applied Microbiology, Volume 15, Issue 5, 1 November...
物理的性質の検討) 充分に行なわれていない安定型無水α-, 無水αβ複合-および無水β-ラクトース結晶について, 融点および溶解度を中心とした検討を行なった. 安定型無水α-ラクトースの常法および示差熱分析 (昇温速度10℃/分) による融点 (分解) はそれぞれ207.7, 216.5℃, 溶解度は5, 15, 25, 35℃ において, それぞれ, 10.1±0.14, 11.5±0.02, 13.9±0.24, 18.5±0.16 (g/100g水) であった. 無水αβ複合ラクトース (α/β比, 4:1) の上記方法による融点はそれぞれ204.0, 205.0℃, 上記と同じ温度における溶解度はそれぞれ17.9±0.5, 15.2±0.47, 17.5±0.23, 20.7±0.45であった. また, 無水β-ラクトースの同じく融点はそれぞれ228.8, 243.0℃, 同じく溶解度はそれぞれ39.9±0.42, 42.6±0.47, 45.0±0.34, 48.3±0.12で, 文献からの計算値よりもかなり低い値を示した. 無水αβ複合ラクトースについては,...