A5029936028- DNA Repair Mechanisms
- Probiotics and Fermented Foods
- Genetics, Aging, and Longevity in Model Organisms
- Microtubule and mitosis dynamics
- Fungal and yeast genetics research
- CRISPR and Genetic Engineering
- Microbial Metabolites in Food Biotechnology
- Gut microbiota and health
- Semiconductor Lasers and Optical Devices
- Photonic and Optical Devices
- Digestive system and related health
- Immune Response and Inflammation
- Genomics and Chromatin Dynamics
- Semiconductor Quantum Structures and Devices
- Synthesis and Characterization of Heterocyclic Compounds
- Analytical Chemistry and Chromatography
- RNA Research and Splicing
- Advanced Fiber Optic Sensors
- Mitochondrial Function and Pathology
- Insect Utilization and Effects
- Animal Diversity and Health Studies
- Neonatal Health and Biochemistry
- Animal health and immunology
- Glycosylation and Glycoproteins Research
- Biochemical effects in animals
Kindai University
1988-2024
Harvard University
2011-2023
Boston VA Research Institute
2021-2023
Tohoku University
2006-2017
Sumitomo Electric Industries (United States)
1999-2012
Osaka University
2001-2010
Nihon University
2008
Sumitomo Electric Industries (Japan)
2005
Tokyo Institute of Technology
2005
Research Institute for Bioscience and Biotechnology
1997-2004
To characterize the adhesion molecule of Lactobacillus plantarum LA 318 that shows high to human colonic mucin (HCM).The test used BIACORE assay where PBS-washed bacterial cells showed a significant decrease in adherence HCM than distilled water-washed cells. A component PBS wash fraction adhered and main protein was detected as c. 40-kDa band using SDS-PAGE. Using homology comparisons N-terminal amino acid sequences compared with sequence databases, this identified...
Homologous recombination (HR) is essential for the repair of blocked or collapsed replication forks and production crossovers between homologs that promote accurate meiotic chromosome segregation. Here, we identify HIM-18, an ortholog MUS312/Slx4, as a critical player required in vivo processing late HR intermediates Caenorhabditis elegans. DNA damage sensitivity accumulation (RAD-51 foci) during premeiotic entry suggest HIM-18 HR–mediated at stalled forks. A reduction crossover...
Abstract Four different SYP proteins (SYP-1, SYP-2, SYP-3, and SYP-4) have been proposed to form the central region of synaptonemal complex (SC) thereby bridging axes paired meiotic chromosomes in Caenorhabditis elegans. Their interdependent localization suggests that they may interact within SC. Our studies reveal for first time how these are organized Yeast two-hybrid co-immunoprecipitation show SYP-1 is only protein capable homotypic interactions, able with both SYP-2 SYP-3 directly,...
The number and distribution of crossover events are tightly regulated at prophase meiosis I. resolution Holliday junctions by structure-specific endonucleases, including MUS-81, SLX-1, XPF-1 GEN-1, is one the main mechanisms proposed for formation. However, how these nucleases coordinately resolve still unclear. Here we identify both functional overlap differences between four regarding their roles in formation control Caenorhabditis elegans germline. We show that but not can bind to...
Lactobacillus acidophilus group and a Lact. reuteri isolated from human faeces were examined for production of antimicrobial agents against 16 strains six species food-borne enteric pathogenic bacteria. Several gasseri showed wide inhibitory activity the tested Gassericin A produced by LA39 was one most widely active bacteriocins. It bactericidal without causing cell lysis.
Breast cancer susceptibility gene 1 (BRCA1) and binding partner BRCA1-associated RING domain protein (BARD1) form an essential E3 ubiquitin ligase important for DNA damage repair homologous recombination. The Caenorhabditis elegans orthologs, BRC-1 BRD-1, also function in repair, recombination, as well meiosis. Using functional GFP fusions we show that mitotically-dividing germ cells BRD-1 are nucleoplasmic with enrichment at foci partially overlap the recombinase RAD-51. Co-localization...
Although the SLX4 complex, which includes structure-specific nucleases such as XPF, MUS81, and SLX1, plays important roles in repair of several kinds DNA damage, function SLX1 germline remains unknown. Here we characterized endonuclease activities Caenorhabditis elegans SLX-1-HIM-18/SLX-4 complex co-purified from human 293T cells determined SLX-1 via analysis slx-1(tm2644) mutants. shows a HIM-18/SLX-4–dependent activity toward replication forks, 5′-flaps, Holliday junctions. slx-1 mutants...
Crossover recombination is essential for generating genetic diversity and promoting accurate chromosome segregation during meiosis. The process of crossover tightly regulated initiated by the formation programmed meiotic DNA double-strand breaks (DSBs). number DSBs around 10-fold higher than crossovers in most species, because only a limited are repaired as Moreover, not randomly distributed. Most located on chromosomal arm regions both centromeres telomeres usually devoid crossovers. Either...
The aims of this study were to evaluate the effectiveness nisin A control growth spore-forming bacteria, Bacillus and Paenibacillus, in chilled high-fat, milk pudding reduce heat treatment improve aroma flavour.Nisin was added containing 5·0 7·5% fat final concentrations 40, 80, 120 240 IU ml(-1). Spores from thuringiensis, cereus Paenibacillus jamilae inoculated into samples at 10 spores ml(-1) prior pasteurization 130°C for 2 s. Milk without inoculation pasteurized using less condition...
In order to isolate meiosis-specific genes in Schizosaccharomyces pombe, we have constructed a subtracted cDNA library enriched clones whose expression is enhanced during meiosis induced by nitrogen starvation. Using northern blot analysis, isolated 31 kinds of was meiosis/sporulation-specific manner. We comprehensively named them meu after meiotic upregulated. The transcription 20 found be dependent on the mei4(+) gene, which encodes factor required for progression meiosis. DNA sequencing...
We have reported previously that novel immunostimulatory sequence (ISS) oligodeoxynucleotide (ODN) BL07S from a probiotic strain of Bifidobacterium longum inhibited immunoglobulin (Ig) E production in vitro. However, whether ISS-ODNs probiotics regulate T helper type 2 (Th2)-polarized immune reactions vivo remains unclear. To evaluate the inhibitory effects ODN on I allergic response, BALB/c mice were injected with or without presence ovalbumin (OVA) days 0 and 14. Serum Ig levels (IgE, IgG1...
Abstract In a previous study of the immunoregulatory properties commensal bacterial DNA, we identified strong immunostimulatory oligodeoxynucleotide (ISS‐ODN) ID35 in genomic DNA Lactobacillus rhamnosus GG (LGG). The observed effects are because unique TTTCGTTT motif located at 5′ end ODN, which is different from previously ISS motifs humans and mice. present study, used an ovalbumin (OVA)‐sensitized mouse model to show that potent suppressor antigen‐specific immunoglobulin E (IgE)...
To identify and characterize a new adhesin-like protein of probiotics that show specific adhesion to human blood group A B antigens.Using the BIACORE assay, cell surface components obtained from four lactobacilli strains adhered antigens was tested. Their showed significant when compared bovine serum albumin (BSA) control. The 1 mol l(-1) GHCl fraction extracted Lactobacillus mucosae ME-340 contained 29-kDa band (Lam29) using SDS-PAGE. N-terminal amino acid sequence homology analysis Lam29...
To isolate lactobacilli from the mucus layer of human intestine and evaluate their adhesion abilities using a BIACORE assay.Thirty strains were isolated normal intestinal tissues conventional plate culture. The identified homology comparisons 16S rDNA sequence to databases as Lactobacillus salivarius (26%), fermentum (13%), gasseri (10%), paracasei (7%), casei (3%), mucosae (3%) plantarum (3%). LA 318 shows highest colonic mucin (HCM) assay at 115.30 +/- 12.37 resonance unit (RU). cell wall...
During meiotic prophase I of the fission yeast Schizosaccharomyces pombe, oscillatory nuclear movement occurs. This promotes homologous chromosome pairing and recombination involves cortical dynein, which plays a pivotal role by generating pulling force with help an unknown dynein anchor. We show that Mcp5, homologue budding anchor Num1, may be this putative mcp5+ is predominantly expressed during prophase, GFP-Mcp5 localizes at cell cortex. Moreover, mcp5Delta strain lacks movement....
In order to evaluate probiotic strains applicable for the beneficial immunomodulation of porcine gut (immunobiotics), we previously developed a intestinal epitheliocyte cell line (PIE cells). Here, transcriptomic studies using PIE cells were performed considering that this information would be valuable understanding mechanisms involved in protective activity immunobiotic strain Lactobacillus jensenii TL2937 against inflammatory damage pigs. addition, those provide criteria selecting...
Significance Recombination and chromosome remodeling are tightly regulated to ensure accurate segregation during meiosis. However, how these important steps remains poorly understood. Here, we show that RAD-51 foci, which form at the sites of meiotic DNA double-strand breaks (DSBs), exhibit a biased distribution toward off-centered positions along Caenorhabditis elegans chromosomes, identify two roles for chromatin-associated protein HIM-17. During early prophase I, HIM-17 regulates...
The study aimed for the complete purification and recharacterization of highly hydrophobic circular bacteriocins, gassericin A reutericin 6.Gassericin 6 were purified to homogeneity using previously described method reverse-phase HPLC with an octyl column eluents aqueous acetonitrile 2-propanol. Mass analysis, N-terminal sequencing bacteriocin assay HPLC-purified bacteriocins showed two had identical seamless structures same m/z value (5651) [M + H](+) both specific activity. D/L-amino acid...