A5003765049- Malaria Research and Control
- Invertebrate Immune Response Mechanisms
- Mosquito-borne diseases and control
- Parasites and Host Interactions
- Drug Transport and Resistance Mechanisms
- Aquaculture disease management and microbiota
- Complement system in diseases
- Studies on Chitinases and Chitosanases
- Research on Leishmaniasis Studies
- Vector-borne infectious diseases
- Antimicrobial Peptides and Activities
- Parasite Biology and Host Interactions
- Venomous Animal Envenomation and Studies
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- Entomopathogenic Microorganisms in Pest Control
- Glycosylation and Glycoproteins Research
- Lipid Membrane Structure and Behavior
- vaccines and immunoinformatics approaches
- Machine Learning in Bioinformatics
- HIV Research and Treatment
- Streptococcal Infections and Treatments
- Bird parasitology and diseases
- Eosinophilic Esophagitis
Kobe University
2014-2024
Hyogo University of Health Sciences
2024
Kobe International University
2015
Ehime University
2005-2012
Tottori University
2009-2012
Azabu University
1998-2002
ABSTRACT Loop-mediated isothermal amplification (LAMP), a novel nucleic acid method, was developed for the clinical detection of four species human malaria parasites: Plasmodium falciparum , P. vivax malariae and ovale . We evaluated sensitivity specificity LAMP in comparison with results microscopic examination nested PCR. showed limit (analytical sensitivity) 10 copies target 18S rRNA genes 100 genus detected parasites 67 68 microscopically positive blood samples (sensitivity, 98.5%) 3 53...
One of the major bottlenecks in malaria research has been difficulty recombinant protein expression. Here, we report application wheat germ cell-free system for successful production proteins. For proof principle, Pfs25, PfCSP, and PfAMA1 proteins were chosen. These genes contain very high A/T sequences are also difficult to express as In our system, native codon-optimized versions Pfs25 produced equal amounts PfCSP without any codon optimization expressed. The products soluble, with yields...
ABSTRACT The aim of a malaria transmission-blocking vaccine is to block the development parasites in mosquito and thus prevent subsequent infection human host. Previous studies have demonstrated that gametocyte/gamete surface protein Pfs230 can induce immunity evaluated Escherichia coli -produced as candidate. In this study, we used wheat germ cell-free expression system produce N-terminal fragments activity antisera raised against recombinant protein. rabbit reacted cultured gametocytes...
The major virulence determinant of the rodent malaria parasite, Plasmodium yoelii , has remained unresolved since discovery lethal line in 1970s. Because this parasite correlates with ability to invade different types erythrocytes, we evaluated potential role erythrocyte binding ligand, Py EBL. We found 1 amino acid substitution a domain responsible for intracellular trafficking between and nonlethal lines and, furthermore, that localization EBL was distinct these lines. Genetic modification...
Nearly 100% protection against malaria infection can be achieved in humans by immunization with P. falciparum radiation-attenuated sporozoites (RAS). Although it is thought that mediated T cell and antibody responses, only a few of the many pre-erythrocytic (sporozoite liver stage) antigens are targeted these responses have been identified.Twenty seven were selected using bioinformatics analysis expression databases expressed wheat germ cell-free protein system. Recombinant proteins...
We identified naturally induced antibodies from malaria patients in Thailand and clarified the effect of on gametocyte development. Fifty-nine percent Plasmodium falciparum-infected blood samples (17 29) fed to female Anopheles mosquitoes showed no oocyst infection. Seventeen (5 distorted morphology hampered maturity gametocytes. A possible mechanism for inhibitory activity was shown by binding plasma live, immature, intraerythrocytic gametocytes during incubation period. One hundred...
Plasmodium parasites cause malaria in mammalian hosts and are transmitted by Anopheles mosquitoes. Gametocytes, which differentiate from asexual-stage parasites, activated environmental changes when ingested into the mosquito midgut, rapidly released erythrocytes prior to fertilization. Secretory proteins localized osmiophilic bodies (OBs), organelles unique gametocytes, have been reported be involved female gametocyte egress. In this study, we investigate dynamics of OBs gametocytes...
A specific galactose-binding lectin was shown to inhibit the hemolytic effect of streptolysin O (SLO), an exotoxin produced by Streptococcus pyogenes. Commercially available lectins that recognize N-acetyllactosamine (ECA), T-antigen (PNA), and Tn-antigen (ABA) agglutinated rabbit erythrocytes, but had no on SLO-induced hemolysis. In contrast, hemolysis inhibited AKL, a purified from sea hare (Aplysia kurodai) eggs recognizes α-galactoside oligosaccharides. This inhibitory blocked...
Glycosphingolipids (GSLs) were purified from adults and plerocercoids of the tapeworm Diphyllobothrium hottai, their chemical structures determined. Total lipid fractions prepared chloroform/methanol extracts whole tissues fractionated successively on ion-exchange chromatography, silicic acid column preparative TLC. The GSLs characterized by methylation analysis, TLC-immunostaining, liquid secondary ion MS, MALDI-TOF 1H-NMR. Ten isolated adult worms four plerocercoids, comprising mono-, di-,...
We report a 20-year-old male with past history of diarrhea and discharge strobila broad tapeworm from his anus 3 months ago. He again noticed passing he visited our hospital the sample. The macroscopic appearance pathological features strongly suggested Diphyllobothriasis. Soon after first visit to hospital, capsule endoscopy was performed result no evidence strobilae or scoleces. Praziquantel at 20 mg/kg orally administered once. Before drug administration, egg-like structures were present...
Plasmodium species cause malaria, and in the instance of falciparum is responsible for a societal burden over 600,000 deaths annually. The symptoms pathology malaria are due to intraerythocytic parasites. Erythrocyte invasion mediated by parasite merozoite stage, accompanied formation parasitophorous vacuolar membrane (PVM), within which develops. apical rhoptry organelle contains various proteins that contribute erythrocyte attachment invasion. RON3, bulb protein, undergoes protein...