A5010796412- Sunflower and Safflower Cultivation
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Peanut Plant Research Studies
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Plant tissue culture and regeneration
- Insect Resistance and Genetics
- Plant Disease Resistance and Genetics
- Insect-Plant Interactions and Control
- Plant Virus Research Studies
- Genetic diversity and population structure
- CRISPR and Genetic Engineering
- Plant Genetic and Mutation Studies
- Plant Disease Management Techniques
- Agricultural pest management studies
- Plant Stress Responses and Tolerance
- Nematode management and characterization studies
- Transgenic Plants and Applications
- Legume Nitrogen Fixing Symbiosis
- Genetically Modified Organisms Research
- Wheat and Barley Genetics and Pathology
- Soybean genetics and cultivation
- RNA and protein synthesis mechanisms
University of Delhi
2015-2024
Defence Food Research Laboratory
2020
Abstract Background Extensive mapping efforts are currently underway for the establishment of comparative genomics between model plant, Arabidopsis thaliana and various Brassica species. Most these studies have deployed RFLP markers, use which is a laborious time-consuming process. We therefore tested efficacy PCR-based Intron Polymorphism (IP) markers to analyze genome-wide synteny oilseed crop, juncea (AABB genome) A. analyzed arrangement 24 (previously described) genomic block segments in...
Brassica juncea var. Varuna is an economically important oilseed crop of family Brassicaceae which vulnerable to abiotic stresses at specific stages in its life cycle. Till date no attempts have been made elucidate genome-wide changes transcriptome against high temperature or drought stress. To gain global insights into genes, transcription factors and kinases regulated by these explore information on coding transcripts that are associated with traits agronomic importance, we utilized a...
Summary Root‐knot nematodes (RKNs, Meloidogyne incognita ) are economically important endoparasites with a wide host range. We used comprehensive transcriptomic approach to investigate the expression of both tomato and RKN genes in roots at five infection time intervals from susceptible plants two resistant plants, grown under soil conditions. Differentially expressed during (1827, tomato; 462, RKN) resistance (25, 160, interactions were identified. In responses, involved cell wall...
Abstract Background MicroRNAs (miRNAs) are ubiquitous components of endogenous plant transcriptome. miRNAs small, single-stranded and ~21 nt long RNAs which regulate gene expression at the post-transcriptional level known to play essential roles in various aspects development growth. Previously, a number have been identified potato through silico analysis deep sequencing approach. However, identification approach was limited few tissue types developmental stages. This study reports...
Micro RNAs (miRNAs) are involved in diverse biological processes including adaptive response towards abiotic stresses. To unravel small and more specifically miRNAs that can potentially regulate determinants of stress tolerance, next generation sequencing B. juncea seedlings subjected to high temperature, salt drought conditions was carried out. With the help UEA sRNA workbench software package, 51 conserved belonging 30 miRNA families were identified. As there limited genomic information...
Carthamus tinctorius L. is a dryland oilseed crop yielding high quality edible oil. Previous studies have described significant phenotypic variability in the and used geographical distribution trait values to develop core collections. However, molecular diversity component was lacking earlier collections thereby limiting their utility breeding programs. The present study evaluated for twelve agronomically important traits during two growing seasons (2011-12 2012-13) global reference...
Carthamus tinctorius L. (safflower) is an important oilseed crop producing seed oil rich in unsaturated fatty acids. Scarcity of identified marker-trait associations a major limitation toward development successful marker-assisted breeding programs safflower. In the present study, safflower panel (CartAP) comprising 124 accessions derived from two core collections was assayed for its suitability association mapping. Genotyping CartAP using microsatellite markers revealed significant genetic...
Safflower (Carthamus tinctorius L.), an Asteraceae member, yields high quality edible oil rich in unsaturated fatty acids and is resilient to dry conditions. The crop holds tremendous potential for improvement through concerted molecular breeding programs due the availability of significant genetic phenotypic diversity. Genomic resources that could facilitate such remain largely underdeveloped crop. present study was initiated develop a large set novel microsatellite markers safflower using...
Root-knot nematodes (RKNs, Meloidogyne spp.) are the most damaging plant parasites causing severe losses to crop production. The present study reports genome-wide identification and characterization of both tomato RKN miRNAs simultaneously from RKN-infected susceptible roots using high-throughput sequencing technique. RNAseq data 11 small RNA libraries derived 5 disease development stages identified 281 novel in addition 52 conserved 4 variants miRNAs. Additionally, same set 38 290 Both...
Limited information is available on abiotic stress-mediated alterations of chromatin conformation influencing gene expression in plants. In order to characterize the effect stresses changes conformation, we employed FAIRE-seq (formaldehyde-assisted isolation regulatory element sequencing) and DNase-seq isolate accessible regions from Arabidopsis thaliana seedlings exposed either heat, cold, salt, or drought stress. Approximately 25% genome were captured as open chromatin, majority which...
Low temperature is a major abiotic stress that impedes plant growth and development. Brassica juncea an economically important oil seed crop sensitive to freezing during pod filling subsequently leading abortion of seeds. To understand the cold mediated global perturbations in gene expression, whole transcriptome B. siliques were exposed sub-optimal was sequenced. Manually self-pollinated at different stages development subjected either short (6 h) or long (12 durations chilling followed by...
Abstract Black gram [ Vigna mungo (L.) Hepper var. ] is a warm‐season legume highly prized for its protein content along with significant folate and iron proportions. To expedite the genetic enhancement of black gram, high‐quality draft genome from center origin crop indispensable. Here, we established sequence an Indian cultivar, ‘Uttara’ (IPU 94‐1), known high resistance to mungbean yellow mosaic virus. Pacific Biosciences California, Inc. (PacBio) single‐molecule real‐time (SMRT) Illumina...
Nymphaea, commonly known as water lily, is the largest and most widely distributed genus in order Nymphaeales. The importance of Nymphaea wetland ecosystems their increased vulnerability make them a great choice for conservation management. In this work, we studied genetic diversity collection 90 N. micrantha 92 nouchali individuals from six different states India, i.e., Assam, Manipur, Meghalaya, Maharashtra, Goa, Kerala, using simple sequence repeat (SSR) markers developed by low...
Mushrooms are macrofungi that serve as a vegetarian source of protein along with various bio-active molecules primary health importance. The activity the range from antioxidant, immunomodulation, hepatoprotection. Cultivated oyster mushrooms also rich in these components which may be estimated quantitatively by skill intensive ‘destructive’ chemical techniques. Infra Red spectroscopy provides non-destructive user friendly technique to quickly assess presence compounds mushroom species used...