A5101723747- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Diabetes Treatment and Management
- Malaria Research and Control
- Animal Virus Infections Studies
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Viral gastroenteritis research and epidemiology
- Plant Virus Research Studies
- SARS-CoV-2 detection and testing
- Salivary Gland Tumors Diagnosis and Treatment
- Insect symbiosis and bacterial influences
- Advanced Glycation End Products research
- Monoclonal and Polyclonal Antibodies Research
- Diabetes and associated disorders
- Liver Disease Diagnosis and Treatment
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Diabetic Foot Ulcer Assessment and Management
- Ear and Head Tumors
- Natural Antidiabetic Agents Studies
- Poxvirus research and outbreaks
- Neuroblastoma Research and Treatments
- CAR-T cell therapy research
Shenzhen Bay Laboratory
2022-2025
Fudan University
2012-2025
Qingdao University
2013-2025
Zhongshan Hospital
2012-2025
The First Affiliated Hospital, Sun Yat-sen University
2012-2025
Sun Yat-sen University
2012-2025
Zhongshan Hospital of Xiamen University
2024-2025
Sichuan University
2009-2024
Beijing Tongren Hospital
2022-2024
Capital Medical University
2022-2024
The B.1.1.7 variant (also known as Alpha) of SARS-CoV-2, the cause COVID-19 pandemic, emerged in UK summer 2020. prevalence this increased rapidly owing to an increase infection and/or transmission efficiency1. Alpha contains 19 nonsynonymous mutations across its viral genome, including 8 substitutions or deletions spike protein that interacts with cellular receptors mediate and tropism. Here, using a reverse genetics approach, we show individual substitutions, only N501Y resulted consistent...
We report that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta spike mutation P681R plays a key role in the Alpha-to-Delta variant replacement during disease 2019 (COVID-19) pandemic. SARS-CoV-2 efficiently outcompetes Alpha human lung epithelial cells and primary airway tissues. The is located at furin cleavage site separates 1 (S1) S2 subunits. Reverting to wild-type P681 significantly reduces replication of level lower than variant. Mechanistically, enhances full-length...
Abstract A high-throughput platform would greatly facilitate coronavirus disease 2019 (COVID-19) serological testing and antiviral screening. Here we present a nanoluciferase severe respiratory syndrome 2 (SARS-CoV-2-Nluc) that is genetically stable replicates similarly to the wild-type virus in cell culture. SARS-CoV-2-Nluc can be used measure neutralizing antibody activity patient sera within 5 hours, it produces results concordance with plaque reduction neutralization test (PRNT)....
Abstract We report a live-attenuated SARS-CoV-2 vaccine candidate with (i) re-engineered viral transcription regulator sequences and (ii) deleted open-reading-frames (ORF) 3, 6, 7, 8 (∆3678). The ∆3678 virus replicates about 7,500-fold lower than wild-type on primary human airway cultures, but restores its replication interferon-deficient Vero-E6 cells that are approved for production. is highly attenuated in both hamster K18-hACE2 mouse models. A single-dose immunization of the protects...
Uric acid (UA) is a risk factor for endothelial dysfunction, process in which inflammation may play an important role. UA increases high mobility group box chromosomal protein 1 (HMGB1) expression and extracellular release cells. HMGB1 inflammatory cytokine that interacts with the receptor advanced glycation end products (RAGE), inducing oxidative stress response, leads to dysfunction. In this study, human umbilical vein cells (HUVECs) were incubated concentration of (20 mg/dL) after...
Abstract We engineered three SARS-CoV-2 viruses containing key spike mutations from the newly emerged United Kingdom (UK) and South African (SA) variants: N501Y UK SA; 69/70-deletion+N501Y+D614G UK; E484K+N501Y+D614G SA. Neutralization geometric mean titers (GMTs) of twenty BTN162b2 vaccine-elicited human sera against mutant were 0.81- to 1.46-fold GMTs parental virus, indicating small effects these on neutralization by elicited two BNT162b2 doses.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the global COVID-19 pandemic infecting more than 106 million people and causing 2.3 deaths. The rapid deployment of antibody-based countermeasures provided hope for curtailing disease ending
Arboviruses maintain high mutation rates due to lack of proofreading ability their viral polymerases, in some cases facilitating adaptive evolution and emergence. Here we show that, just before its 2013 spread the Americas, Zika virus (ZIKV) underwent an envelope protein V473M substitution (E-V473M) that increased neurovirulence, maternal-to-fetal transmission, viremia facilitate urban transmission. A preepidemic Asian ZIKV strain (FSS13025 isolated Cambodia 2010) engineered with...
Zika virus (ZIKV) is an emerging mosquito-transmitted flavivirus that shares a considerable degree of homology with dengue (DENV). Here, we examined longitudinal antibody response against ZIKV during natural infection in 2 convalescent individuals. By decomposing the recognition into DI/DII and DIII E glycoprotein, showed their development humans followed spatiotemporal hierarchy. Plasma binding to appeared peak wane early extensive cross-reactivity DENV. Binding DIII, however, peaked but...
Abstract Beginning in the summer of 2020, a variant SARS-CoV-2, cause COVID-19 pandemic, emerged United Kingdom (UK). This B.1.1.7 increased rapidly prevalence among sequenced strains, attributed to an increase infection and/or transmission efficiency. The UK has 19 nonsynonymous mutations across its viral genome including 8 substitutions or deletions spike protein, which interacts with cellular receptors mediate and tropism. Here, using reverse genetics approach, we show that, individual...
A spike protein mutation D614G became dominant in SARS-CoV-2 during the COVID-19 pandemic. However, mutational impact on viral spread and vaccine efficacy remains to be defined. Here we engineer USA-WA1/2020 strain characterize its effect replication, pathogenesis, antibody neutralization. The significantly enhances replication human lung epithelial cells primary airway tissues, through an improved infectivity of virions with receptor-binding domain "up" conformation for binding ACE2...
Abstract Zika virus (ZIKV) emerged from obscurity in 2013 to spread Asia the South Pacific and Americas, where millions of people were infected, accompanied by severe disease including microcephaly following congenital infections. Phylogenetic studies have shown that ZIKV evolved Africa later Asia, Asian lineage is responsible for recent epidemics Americas. However, reasons sudden emergence remain enigmatic. Here we report evolutionary analyses revealed four mutations, which occurred just...
NOTCH2NLC-related neuronal intranuclear inclusion disease (NIID) is a neurodegenerative with characteristic white matter lesions (WMLs) visible on MRI. However, the distribution of WMLs and their clinical correlations remain poorly understood in NIID. This study aims to investigate spatial temporal brain patients We retrospectively evaluated diagnosed NIID Zhongshan Hospital, Fudan University. Detailed information, including retrospective MRI data, was collected. Spatial fluid-attenuated...
The global outbreak of monkeypox virus (MPXV), combined with the termination smallpox vaccination and lack specific antiviral treatments, raises increasing concerns. surface proteins M1R B6R MPXV are crucial for transmission serve as key targets vaccine development. In this study, a panel human antibodies targeting is isolated from antibody library using phage display technology. Among these antibodies, A138 against B026 show most potent broad-spectrum neutralizing activities Vaccinia...