Even after decades of research, the mechanism neurodegeneration remains understudied, hindering discovery effective treatments for neurodegenerative diseases. Recent reports suggest that ferroptosis could be a novel therapeutic target While polyunsaturated fatty acid (PUFA) plays an important role in and ferroptosis, how PUFAs may trigger these processes largely unknown. PUFA metabolites from cytochrome P450 epoxide hydrolase metabolic pathways modulate neurodegeneration. Here, we test...

Wrch-1, an atypical and transforming Rho GTPase, regulates cellular activities including proliferation actin organization, but its functions effectors remain poorly characterized. We show here that Wrch-1 distributes along the apical basolateral membranes in MDCK cells binds cell polarity protein Par6 a GTP-dependent manner. Activated negatively kinetics of tight junction (TJ) assembly during epithelial polarization has no detectable effect on overall confluent monolayers. It also causes...

Activation of the mammalian Ras-Raf-MEK-ERK MAPK signaling cascade promotes cellular proliferation, and activating Ras mutations are implicated in cancer onset maintenance. This pathway, a therapeutic target interest, is highly conserved required for vulval development C. elegans. Gain-of-function ortholog lead to constitutive pathway multivulva (Muv) phenotype. MCP compounds were identified yeast two-hybrid screen their ability disrupt Ras-Raf interactions. However, this had not been...

Previous studies described functional roles for Rho GDP dissociation inhibitor 2 (RhoGDI2) in bladder, gastric and breast cancers. However, only limited expression no analyses have been done RhoGDI2 ovarian cancer. We determined protein function First, gel blot analysis was performed to determine the levels of cells lines. but not RhoGDI1 varied widely carcinoma cell lines, with elevated seen Ras-transformed epithelial cells. Next, immunohistochemistry detect patient samples cysts cancer...

Adenylyl cyclase type 6 (AC6) activity is inhibited by protein kinase C (PKC) in vitro; however, intact cells, PKC activation does not inhibit the of transiently expressed AC6. To investigate effects on AC6 we constructed human embryonic kidney (HEK) 293 cells that stably express wild-type (AC6-WT) or an mutant lacking a and cyclic AMP-dependent (PKA) phosphorylation site, Ser674 (AC6-S674A). In contrast to vitro observations, observed PKC-mediated enhancement forskolin-...

Wrch-1 is an atypical Rho family small GTPase with roles in migration, epithelial cell morphogenesis, osteoclastogenesis, and oncogenic transformation. Here, we observed rapid relocalization of from the plasma membrane upon serum stimulation. Studies revealed a requirement for serum-stimulated tyrosine phosphorylation at residue Y254 within its C-terminal targeting domain, mediated by nonreceptor kinase Src. Genetic or pharmacological loss Src activity blocked both Wrch-1. Functionally, was...

Rho GTPases are key regulators of cellular protrusion and involved in many developmental events including axon guidance during nervous system development. GTPase pathways display functional redundancy events, guidance. Therefore, their roles can often be masked when using simple loss-of-function genetic approaches. As a complement to genetics, we constructed constitutively activated CDC-42(G12V) expressed C. elegans neurons. drove the formation ectopic lamellipodial filopodial protrusions...

During development, neuronal cells extend an axon toward their target destination in response to a cue form properly functioning nervous system. Rho proteins, Ras-related small GTPases that regulate cytoskeletal organization and dynamics, cell adhesion, motility, are known guidance. Despite extensive knowledge about canonical proteins (RhoA/Rac1/Cdc42), little is the Caenorhabditis elegans (C. elegans) atypical Cdc42-like family members CHW-1 CRP-1 regards pathfinding migration....

Even after decades of research, the mechanism neurodegeneration remains understudied, hindering discovery effective treatments for neurodegenerative diseases. Recent reports suggest that ferroptosis could be a novel therapeutic target While polyunsaturated fatty acid (PUFA) plays an important role in and ferroptosis, how PUFAs may trigger these processes largely unknown. PUFA metabolites from cytochrome P450 epoxide hydrolase metabolic pathways modulate neurodegeneration. Here, we test...

Polyunsaturated fatty acids (PUFAs) and non-steroidal anti-inflammatory drugs (NSAIDs) show anticancer activities through diverse molecular mechanisms. However, the capacities of either PUFAs or NSAIDs alone is limited. We examined whether combining with docosahexaenoic (DHA), commonly derived from fish oils, would possibly synergize their activity. determined viability lung cancer cell lines (NCI-H1573, A549, NCI-H1299, NCI-H1975) after exposure to DHA various NSAIDs. further conducted...

Rho proteins are part of the Ras superfamily, which function to modulate cytoskeletal dynamics including cell adhesion and motility. Recently, an activating mutation in Cdc42, a family GTPase, was found patient sample melanoma. Previously, our work had shown PI3K important downstream mutationally active Cdc42. Our present study sought determine whether crucial partner for Cdc42 melanoma cells line with BRAF mutation, is most common cutaneous In this we were able show that contributes...

This study aims to uncover potent cytochrome P450 (CYP) and epoxide hydrolase (EH) metabolites implicated in Aβ and/or tau-induced neurodegeneration, independent of neuroinflammation, by utilizing

This presentation explores the molecular interplay between cytochrome P450-epoxide hydrolase (CYP-EH) metabolism of polyunsaturated fatty acid (PUFA) and neurodegeneration. While PUFA CYP-EH metabolites are key lipid mediators for physiological processes, their role in neurodegeneration remains understudied due to complexity enigmatic nature mechanism The central hypothesis this project is that plays a critical To test efficiently, used Caenorhabditis elegans (C. elegans) as simple model...

Abstract A virtual addiction medicine elective was developed using interactive multimedia modules, active learning strategies, and patient-based cases. Student had opportunities for professional networking interacting with physicians patients. The successful in boosting Year-1 medical students’ confidence to screen, manage, treat patients substance abuse disorder.

Abstract During development, neuronal cells extend an axon towards their target destination in response to a cue form properly functioning nervous system. Rho proteins, Ras-related small GTPases that regulate cytoskeletal organization and dynamics, cell adhesion, motility, are known guidance. Despite extensive knowledge about canonical proteins (RhoA/Rac1/Cdc42), little is the Caenorhabditis elegans ( C. ) atypical Cdc42-like family members CHW-1 CRP-1 regards pathfinding migration. chw-1...

Ras-related C3 botulinum toxin substrate (Rac1) and Cell division control protein 42 (Cdc42) GTPases play critical roles in cancer cell metastasis. Omega-3 polyunsaturated fatty acids (PUFAs) non-steroidal anti-inflammatory drugs (NSAIDs) show anticancer activities through diverse molecular mechanisms. We have already demonstrated that combining NSAIDs with PUFAs was more effective at altering the expression of proteins RAS/MEK/ERK PI3K/Akt pathways. studied ability selected omega-3...

Abstract Aging is one of the major risk factors for many chronic diseases, including diabetes, neuropathy, hypertension, cancer, and neurodegenerative diseases. However, mechanism behind aging how affects a variety disease progression remains unknown. Recent research demonstrated cytochrome P450 (CYP)-epoxide hydrolase (EH) metabolites polyunsaturated fatty acids (PUFAs) play critical role in abovementioned age-associated Therefore, could affect diseases by modulating CYP-EH PUFA metabolism....