A5077319422- Liver physiology and pathology
- Pancreatic function and diabetes
- Growth Hormone and Insulin-like Growth Factors
- Pluripotent Stem Cells Research
- Respiratory and Cough-Related Research
- Immune Cell Function and Interaction
- Organ Transplantation Techniques and Outcomes
- Digestive system and related health
- Pharmaceutical studies and practices
- T-cell and B-cell Immunology
- Cancer Cells and Metastasis
- Renal Transplantation Outcomes and Treatments
- Metabolism and Genetic Disorders
- Asthma and respiratory diseases
- Renal and related cancers
- Biomedical and Chemical Research
- Reproductive Biology and Fertility
- Transplantation: Methods and Outcomes
- Viral Infections and Immunology Research
- Immunotherapy and Immune Responses
- 3D Printing in Biomedical Research
Cincinnati Children's Hospital Medical Center
1992-2023
University of Cincinnati
2023
Background & AimsPreclinical identification of compounds at risk causing drug induced liver injury (DILI) remains a significant challenge in development, highlighting need for predictive human system to study complicated DILI mechanism and susceptibility individual drug. Here, we established organoid (HLO)–based screening model analyzing pathology resolution.MethodsWe first developed reproducible method generate HLO from storable foregut progenitors pluripotent stem cell (PSC) lines with...
Changes in symptom score and peak expiratory flow (PEF) the use of medications during first acute exacerbation asthma were studied 41 patients matched controls who took part a panel study. An was defined as presence at least one following: any unscheduled physician visit, visit to an emergency room, or hospitalization for treatment asthma; decrease PEF by 30% from patient's best reading; increase symptoms day night over 48 h not responding usual medications; commencement doubling dose oral...
Timely controlled oxygen (O2) delivery is crucial for the developing liver. However, influence of O2 on intercellular communication during hepatogenesis unclear. Using a human induced pluripotent stem cell-derived liver bud (hiPSC-LB) model, we found hypoxia with an O2-permeable plate promoted hepatic differentiation accompanied by TGFB1 and TGFB3 suppression. Conversely, extensive generated O2-non-permeable elevated TGFBs cholangiocyte marker expression. Single-cell RNA sequencing revealed...
Significant evidence suggests a connection between transplant rejection and the presence of high levels pre-existing memory T cells. Viral infection can elicit viral-specific cells that cross-react with allo-MHC capable driving allograft in mice. Despite these advances, despite their critical role rejection, systematic study allo-reactive cells, specificities, cross-reactivity viral antigens has not been performed. Here, we established model to identify, isolate, characterize cross-reactive...
FSH, which stimulates cAMP in the Sertoli cell, markedly lowers concentration of insulin-like growth factor-binding protein-3 (IGFBP-3) cell-conditioned medium; contrast, factor-I (IGF-I) increases BP-3 expression. In this study, mechanisms controlling contrasting effects and IGF-I were investigated. The abundance mRNA was dramatically lowered by (Bu)2cAMP, but unaffected IGF-I. Analyzed ligand blot conditioned medium, coincubation (Bu)2cAMP largely eliminated increase observed with alone....
This study was designed to test the hypothesis that children with atopic dermatitis and short stature fail release growth hormone after falling asleep. Peak serum response arginine compared peak concentration during sleep in 6 (greater than 3 SD below mean) aged 7 12 years, 5 control 9 years without or asthma but unexplained mean). All achieved normal levels of asleep, whereas did not. patients were capable releasing stimulation. The results suggest some prepubertal there may be an...
Abstract Following their proliferative expansion and differentiation into effector cells like Th1, Tfh, T central memory precursors (Tcmp), most CD4+ die, while some survive become cells. Here, we explored how Bcl-2 family members controlled the survival of during distinct phases mouse acute LCMV infection. During expansion, found that Th1 dominated response, downregulated expression Bcl-2, did not require for survival. Instead, they relied on anti-apoptotic protein, A1 Similarly, Th17 in an...