A5049996926- Pluripotent Stem Cells Research
- Liver physiology and pathology
- Renal and related cancers
- Acute Myeloid Leukemia Research
- Complement system in diseases
- Pancreatic function and diabetes
- COVID-19 Clinical Research Studies
- Cell Adhesion Molecules Research
- Immunodeficiency and Autoimmune Disorders
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Hematopoietic Stem Cell Transplantation
- Metabolism and Genetic Disorders
- RNA modifications and cancer
- Blood disorders and treatments
- Renal Diseases and Glomerulopathies
- Erythrocyte Function and Pathophysiology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Single-cell and spatial transcriptomics
- 3D Printing in Biomedical Research
- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- Global Health and Surgery
- Anesthesia and Neurotoxicity Research
- Erythropoietin and Anemia Treatment
Kyoto University
2015-2024
Tokyo Medical and Dental University
2019-2024
The University of Tokyo
2019-2020
Yokohama City University
2019
Background & AimsPreclinical identification of compounds at risk causing drug induced liver injury (DILI) remains a significant challenge in development, highlighting need for predictive human system to study complicated DILI mechanism and susceptibility individual drug. Here, we established organoid (HLO)–based screening model analyzing pathology resolution.MethodsWe first developed reproducible method generate HLO from storable foregut progenitors pluripotent stem cell (PSC) lines with...
Abstract Obtaining highly purified differentiated cells via directed differentiation from human pluripotent stem (hPSCs) is an essential step for their clinical application. Among the various conditions that should be optimized, precise role and contribution of extracellular matrix (ECM) during are relatively unclear. Here, using a short fragment laminin 411 (LM411-E8), ECM predominantly expressed in vascular endothelial basement membrane, we demonstrate switching defined ECMs robustly...
Nakajo-Nishimura syndrome (NNS) is an immunoproteasome-associated autoinflammatory disorder caused by a mutation of the PSMB8 gene. Although dysfunction immunoproteasome causes various cellular stresses attributed to overproduction inflammatory cytokines and chemokines in NNS, underlying mechanisms autoinflammation are still largely unknown. To investigate understand signal pathways we established panel isogenic pluripotent stem cell (PSC) lines with mutation. Activity PSMB8-mutant...
Heterogeneity among both primed and naive pluripotent stem cell lines remains a major unresolved problem. Here we show that expressing the maternal-specific linker histone H1FOO fused to destabilizing domain (H1FOO-DD), together with OCT4, SOX2, KLF4, LMYC, in human somatic cells improves quality of reprogramming pluripotency. H1FOO-DD expression was associated altered chromatin accessibility around pluripotency genes suppression innate immune response. Notably, generates induced lower...
AK2 is an adenylate phosphotransferase that localizes at the intermembrane spaces of mitochondria, and its mutations cause a severe combined immunodeficiency with neutrophil maturation arrest named reticular dysgenesis (RD). Although dysfunction hematopoietic stem cells (HSCs) has been implicated, earlier developmental events affect fate HSCs and/or progenitors have not reported. Here, we used RD-patient-derived induced pluripotent (iPSCs) as model AK2-deficient human cells. Hematopoietic...
Abstract The induction of tissue-specific vessels in vitro living tissue systems remains challenging. Here, we directly differentiated human pluripotent stem cells into CD32b + putative liver sinusoidal progenitors (iLSEP) by dictating developmental pathways. By devising an inverted multilayered air-liquid interface (IMALI) culture, hepatic endoderm, septum mesenchyme, arterial and quadruple self-organized to generate sustain hepatocyte-like neighbored divergent endothelial subsets composed...
The potential of extrapulmonary ventilation pathways remains largely unexplored. Here, we assessed the enteral approach in hypoxic porcine models under controlled mechanical ventilation. 20 mL/kg oxygenated perfluorodecalin (O2-PFD) was intra-anally delivered by a rectal tube. We simultaneously monitored arterial and pulmonary blood gases every 2 min up to 30 determine gut-mediated systemic venous oxygenation kinetics. Intrarectal O2-PFD administration significantly increased partial...