A5009833598- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Respiratory viral infections research
- Immune Response and Inflammation
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Complement system in diseases
- Animal Disease Management and Epidemiology
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- interferon and immune responses
- Genetic Neurodegenerative Diseases
- Monoclonal and Polyclonal Antibodies Research
- Calcium signaling and nucleotide metabolism
- CRISPR and Genetic Engineering
- Parkinson's Disease and Spinal Disorders
- Advanced Chemical Sensor Technologies
- Poxvirus research and outbreaks
- Olfactory and Sensory Function Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Pluripotent Stem Cells Research
- Systemic Lupus Erythematosus Research
- COVID-19 epidemiological studies
Shiga University of Medical Science
2020-2024
Hokkaido University
2021
We examined the histopathological changes in olfactory mucosa of cynomolgus and rhesus macaque models SARS-CoV-2 infection. infection induced severe inflammatory mucosa. A major histocompatibility complex (MHC) class II molecule, HLA-DR was expressed macrophage supporting cells, melanocytes were increased Supporting cells neurons infected, N protein detected axons bulbs. Viral RNA bulbs brain tissues. The epithelium-olfactory bulb pathway may be important as a route for intracranial by SARS-CoV-2.
As long as the coronavirus disease-2019 (COVID-19) pandemic continues, new variants of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) with altered antigenicity will emerge. The development vaccines that elicit robust, broad, and durable protection against SARS-CoV-2 is urgently required. We have developed a vaccine consisting attenuated vaccinia virus Dairen-I (DIs) strain platform carrying S gene (rDIs-S). rDIs-S induced neutralizing antibody T-lymphocyte responses in...
The use of therapeutic neutralizing antibodies against SARS-CoV-2 infection has been highly effective. However, there remain few practical viruses that are acquiring mutations. In this study, we created 494 monoclonal from patients with COVID-19-convalescent, and identified exhibited the comparable ability to clinically used in neutralization assay using pseudovirus authentic virus including variants concerns. These have different profiles various mutations, which were confirmed by...
In 2020, two mRNA-based vaccines, encoding the full length of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein, have been introduced for control disease (COVID-19) pandemic 1,2 . However, reactogenicity, such as fever, caused by innate immune responses to vaccine formulation remains be improved. Here, we optimized a lipid nanoparticle (LNP)-based mRNA candidate, SARS-CoV-2 protein receptor-binding domain (LNP-mRNA-RBD), which showed improved immunogenicity removing...
Attention has been paid to H5N6 highly pathogenic avian influenza virus (HPAIV) because of its heavy burden on the poultry industry and human mortality. Since an A carrying N6 neuraminidase (NA) never spread in humans, potential for HPAIV cause disease humans efficacy antiviral drugs against need be urgently assessed. We used nonhuman primates elucidate pathogenesis as well determine virus. infection led high fever cynomolgus macaques. The lung injury caused by was severe, with diffuse...
Influenza remains a significant global public health burden, despite substantial annual vaccination efforts against circulating virus strains. As result, novel vaccine approaches are needed to generate long-lasting and universal broadly cross-reactive immunity distinct influenza strains subtypes. Several new candidates currently under development and/or in clinical trials. The successful of vaccines requires testing animal models, other than mice, which capture the complexity human immune...
Tumorigenicity of induced pluripotent stem cells (iPSCs) is anticipated when derived from iPSCs are transplanted. It has been reported that formed a teratoma in vivo autologous transplantation nonhuman primate model without immunosuppression. However, there no study on tumorigenicity major histocompatibility complex (MHC)-matched allogeneic iPSC with immune-competent hosts. To examine the iPSCs, we generated four clones carrying homozygous haplotype MHC. Two were female fibroblasts by using...
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H7N9 highly pathogenic avian influenza virus (HPAIV) infection in a human was first reported 2017. A/duck/Japan/AQ-HE29-22/2017 (H7N9) (Dk/HE29-22), found imported duck meat at an airport Japan, possesses hemagglutinin with multibasic cleavage site, indicating high pathogenicity chickens, as the case of other H7 HPAIVs. In present study, we examined Dk/HE29-22 and effectiveness cap-dependent endonuclease inhibitor (baloxavir) neuraminidase inhibitors (oseltamivir zanamivir) against this...
Macaques are useful animal models for studying the pathogenesis of rheumatoid arthritis (RA) and development anti‐rheumatic drugs. The purpose this study was to identify major histocompatibility complex (MHC) polymorphisms associated with pathology collagen‐induced (CIA) anti‐collagen IgG induction in a cynomolgus macaque model, as MHC affect onset CIA other models. Nine female Filipino macaques were immunized bovine type II collagen (b‐CII) induce CIA, which diagnosed clinically by scoring...
Among inactivated influenza vaccines, the whole virus particle vaccine (WPV) elicits superior priming responses to split (SV) in efficiently inducing humoral and cellular immunity. However, there is concern for undesired adverse events such as fever WPV due its potent immunogenicity. Therefore, this study investigated febrile response induced by subcutaneous injection with quadrivalent vaccines of good manufacturing grade pharmaceutical or investigational products cynomolgus macaques. Body...
Abstract Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infectious disease (COVID-19) has been threatening the world because of severe symptoms and relatively high mortality. To develop vaccines antiviral drugs for COVID-19, an animal model SARS-CoV-2 infection is required to evaluate efficacy prophylactics therapeutics in vivo . Therefore, we examined pathogenicity cynomolgus macaques until 28 days after virus inoculation present study. Cynomolgus showed body temperature rises...
Abstract As long as the coronavirus disease 2019 (COVID-19) pandemic continues, new variants of severe acute respiratory syndrome 2 (SARS-CoV-2) with altered antigenicity will emerge. The development vaccines that elicit robust, broad, and durable protection against SARS-CoV-2 is urgently needed. We have developed a vaccine (rDIs-S) consisting attenuated vaccinia virus DIs strain platform carrying S gene. rDIs-S induced neutralizing antibody T-lymphocyte responses in cynomolgus macaques...
Summary The use of therapeutic neutralizing antibodies against SARS-CoV-2 infection has been highly effective. However, there remain few practical viruses that are acquiring mutations. In this study, we created 494 monoclonal from COVID-19–convalescent patients, and identified exhibited comparable ability to clinically used in the neutralization assay using pseudovirus authentic virus including variants concerns. These have different profiles various mutations, which were confirmed by...