A5101838689- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Software-Defined Networks and 5G
- Electrocatalysts for Energy Conversion
- Microfluidic and Capillary Electrophoresis Applications
- Cloud Computing and Resource Management
- Caching and Content Delivery
- Recycling and utilization of industrial and municipal waste in materials production
- Metallic Glasses and Amorphous Alloys
- Advanced battery technologies research
- Fuel Cells and Related Materials
- Wastewater Treatment and Nitrogen Removal
- Network Security and Intrusion Detection
- Advanced Photocatalysis Techniques
- Cooperative Communication and Network Coding
- Food composition and properties
- Quasicrystal Structures and Properties
- Opportunistic and Delay-Tolerant Networks
- Microbial Fuel Cells and Bioremediation
- Wireless Communication Security Techniques
- Analog and Mixed-Signal Circuit Design
- Coal and Its By-products
- Nuclear materials and radiation effects
- Combustion and Detonation Processes
- Zeolite Catalysis and Synthesis
Nanjing University of Chinese Medicine
2022-2025
Shanghai Jiao Tong University
2011-2024
Fudan University
2023-2024
State Key Laboratory of Genetic Engineering
2023-2024
University of South China
2024
China University of Geosciences
2024
Beijing Institute of Technology
2024
City University of Hong Kong
2024
South China University of Technology
2023-2024
Suzhou University of Science and Technology
2022-2024
Femtogram proteomics: An ultrasensitive capillary zone electrophoresis–mass spectrometry system that is based on an improved nanospray interface has been developed. This used for the analysis of picogram to femtogram amounts E. coli digests; example, over 100 proteins were identified from 16 pg digests by tandem mass spectrometry. AMTs=accurate and time tags. As a service our authors readers, this journal provides supporting information supplied authors. Such materials are peer reviewed may...
P2X receptors are ATP-activated cation channels, and the P2X4 subtype plays important roles in immune system central nervous system, particularly neuropathic pain. Therefore, of increasing interest as potential drug targets. Here, we report cryo-EM structures zebrafish receptor complex with two subtype-specific antagonists, BX430 BAY-1797. Both antagonists bind to same allosteric site located at subunit interface top extracellular domain. Structure-based mutational analysis by...
Abstract Capillary zone electrophoresis (CZE)–tandem mass spectrometry (MS/MS) has recently attracted attention as a tool for shotgun proteomics. However, its performance this analysis so far fallen below that of reversed‐phase liquid chromatography (RPLC)–MS/MS. The use CZE method with wide separation window (up to 90 min) and high peak capacity (ca. 300) is reported. This was coupled an Orbitrap Fusion spectrometer through electrokinetically pumped sheath‐flow interface the complex...
In the Software Defined Network (SDN) ecosystem, controller remains cornerstone of architecture and critical point its success. That is why performance concerns have existed throughout history SDN development. This paper aims at making a reality check on current achieved by mainstream open source controllers. The measurements are carried out in controlled environment, where each tested with own optimized configuration, one hand allowing measuring peak performance, while, other obtaining fair...
Abstract As a new networking paradigm, opportunistic communications have great vision in animal migration tracking, mobile social networking, network remote areas and intelligent transportation, so on. Opportunistic networks are one of the evolutionary ad hoc networks, whose communication links often suffer from frequent disruption long delays. Therefore, many forwarding protocols present major security issues, design faces serious challenges such as how to effectively protect data...
We used reversed-phase liquid chromatography to separate the yeast proteome into 23 fractions. These fractions were then analyzed using capillary zone electrophoresis (CZE) coupled a Q-Exactive HF mass spectrometer an electrokinetically pumped sheath flow interface. The parameters of first optimized for top-down proteomics mixture seven model proteins; we observed that intact protein mode with trapping pressure 0.2 and normalized collision energy 20% produced highest signals most...
Top-down proteomics offers the potential for full protein characterization, but many challenges remain this approach, including efficient separations and effective fragmentation of intact proteins. Capillary zone electrophoresis (CZE) has shown great separation proteins, especially differentially modified proteoforms same gene product. To date, however, CZE been used only with collision-based methods. Here we report first implementation electron transfer dissociation (ETD) online top-down...
Capillary zone electrophoresis (CZE) with an electrokinetically pumped sheath-flow nanospray interface was coupled a high-resolution Q-Exactive mass spectrometer for the analysis of culture filtrates from Mycobacterium marinum. We confidently identified 22 gene products wildtype M. marinum secretome in single CZE-tandem spectrometry (MS/MS) run. A total 58 proteoforms were observed post-translational modifications including signal peptide removal, N-terminal methionine excision, and...
The present study aims to explore the effects of grandiose and vulnerable narcissism on emotion dysregulation examine mediating role self‐esteem these associations. Undergraduates ( N = 426) completed self‐report measurements narcissism, dysregulation. Correlation analyses indicated that was negatively correlated with dysregulation, while positively Moreover, mediational revealed fully mediated association between partially highlights importance deepens understanding associations two forms
ABSTRACT Raspberry ketone is an aromatic phenolic natural product with a variety of bioactivities. To discover novel fungicide molecules based on raspberry ketone, total 25 ester derivatives were synthesized, and their chemical structures well characterized. Among them, most compounds have not been reported except for 3a 3n . Compound 3f exhibited the significant antifungal activity against Valsa mali EC 50 16.430 µM, indicating that its was 234.6 folds more potent than (EC : 3854.401 µM)....