A5032126282- Bacterial Infections and Vaccines
- Microbial Community Ecology and Physiology
- Antibiotic Resistance in Bacteria
- Peripheral Neuropathies and Disorders
- Marine and coastal ecosystems
- Infective Endocarditis Diagnosis and Management
- Bacterial biofilms and quorum sensing
- Ion channel regulation and function
- Enterobacteriaceae and Cronobacter Research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Streptococcal Infections and Treatments
- Hepatitis B Virus Studies
- Infectious Encephalopathies and Encephalitis
- Polar Research and Ecology
- Vaccine Coverage and Hesitancy
- Pneumonia and Respiratory Infections
- Herpesvirus Infections and Treatments
- Antibiotics Pharmacokinetics and Efficacy
- Antimicrobial Resistance in Staphylococcus
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial infections and disease research
- Water Treatment and Disinfection
- Viral Infections and Immunology Research
- Otolaryngology and Infectious Diseases
- Diphtheria, Corynebacterium, and Tetanus
National Institute of Infectious Diseases
2003-2023
National Institutes of Health
2006-2010
National Institute of Health Sciences
1996-1997
National Institute of Health
1986-1989
Tokyo Metropolitan University
1980-1986
ABSTRACT Plasmid-mediated Qnr and AAC(6′)-Ib-cr have been recognized as new molecular mechanisms affecting fluoroquinolone (FQ) resistance. C316, an Escherichia coli strain demonstrating resistance to various FQs, was isolated in Japan. Resistance FQs augmented E. CSH2 transconjugant, but PCR failed detect qnr genes, suggesting the presence of novel plasmid-mediated FQ mechanisms. Susceptibility tests, DNA manipulation, analyses gene its product were performed characterize genetic...
Of 1,456 strains isolated from 2001 to 2003 demonstrating resistance either oxyimino-cephalosporin, 317 strains, in 57 of 132 clinical facilities, were found harbor bla(CTX-M) genes by PCR. Fifty-seven, 161, and 99 harbored belonging the bla(CTX-M-1), bla(CTX-M-2), bla(CTX-M-9) clusters, respectively.
A carbapenem-resistant Serratia marcescens strain, 10mdr148, was identified in a Japanese hospital 2010. The carbapenem resistance of this strain attributed to the production novel metallo-β-lactamase (MBL), named SMB-1 (Serratia metallo-β-lactamase). possessed zinc binding motif, H(Q)XHXDH (residues 116 121), H196, and H263 categorized as member subclass B3 MBL. has 75% amino acid identity with most closely related MBL, AMO1, uncultured bacterium, recently through metagenomic analysis apple...
Abstract To investigate the exact isolation frequency of 16S rRNA methylase–producing, gram-negative pathogenic bacteria, we tested 87,626 clinical isolates from 169 hospitals. Twenty-six strains 16 hospitals harbored methylase genes, which suggests sparse but diffuse spread pan-aminoglycoside–resistant microbes in Japan.
The complete 41 268 bp nucleotide sequence of the IncP-1 β plasmid pBP136 from human pathogen Bordetella pertussis , primary aetiological agent whooping cough, was determined and analysed. This carried a total 46 ORFs: 44 ORFs corresponding to genes in conserved backbone, 2 similar XF1596 XF1597 with unknown function plant Xylella fastidiosa . Interestingly, had no accessory carrying genetic traits such as antibiotic or mercury resistance and/or xenobiotic degradation. Moreover, only two kle...
ABSTRACT Antigenic divergence has been found between Bordetella pertussis vaccine strains and circulating in several countries. In the present study, we analyzed B. isolates collected Japan from 1988 to 2001 using pulsed-field gel electrophoresis (PFGE) sequencing of two virulence-associated proteins. The 107 were classified into three major groups by PFGE analysis; 87 (81%) type A, 19 (18%) B, 1 (1%) was C. Sequence analysis S1 subunit toxin ( ptxS1 ) adhesion pertactin prn genes revealed...
Abstract The Quellung reaction provides a standard means for serotyping Streptococcus pneumoniae , but it requires microscopic examination with skillful technique. We have developed an improved agglutination method anti‐rabbit IgG‐coated latex particles, which are sensitized pooled antisera serotyping/serogrouping S. . Our is as specific and sensitive the test, much easier to perform.
To clarify the relationship between epidemics of severe invasive group A streptococcal infections (streptococcal Toxic Shock-Like Syndrome; TSLS) and common in Japan, we examined T serotypes S. pyogenes strains (group streptococci) isolated from clinical specimens (17999 cases) period 1990–5, including (TSLS) (29 1992–5. Characteristic points analyses were: (1) dominant these periods were T12, T4, T1, T28 TB3264, which consistently isolated; (2) isolates T3 rapidly increased through 1990 to...
We performed a comparative molecular analysis on three types of clinically isolated Acinetobacter spp.: epidemic sequence (STs) baumannii (epidemic ST-AB), non-epidemic A. (non-epidemic ST-AB) and non- spp. A total 87 isolates – 46 , 25 pittii 16 nosocomialis from 43 hospitals were analysed. Of these, 31 ST1 or ST2 according to the Pasteur Institute multilocus typing scheme defined as ST-AB. The other 15 ST-AB harboured bla OXA-23-like gene had an IS Aba1 element upstream OXA-51-like both,...
We examined the major pathogenic substances of Bordetella pertussis for ability to induce nitric oxide, and important biological function macrophages, via gamma interferon in spleen cells. B. pertussis, which produces a variety substances, including toxin filamentous hemagglutinin, causes severe respiratory disease. Nitric oxide was detected culture fluid cells stimulated with or its B oligomer but not A protomer hemagglutinin. Incubation peritoneal exudate macrophages toxin, oligomer,...
Diphtheria tetanus acellular pertussis combined (DTaP) vaccines have been successfully used in Japan by controlling their potencies and toxicities with animal models. In accordance the recent practical introduction of DTaP various formulations, a question has raised other nations as to efficacy quality control system based on tests standard preparations. The World Health Organization issued its guidelines production 1998 along concept ensuring that lots were consistent clinical trial lots,...
Abstract The planktonic bacterial populations in the surface water of hypertrophic Himon‐ya Pond were separated into four fractions (>35 μm, 35‐5 5–1 and <1 μm) by size fractionation suspended particles water. seasonal variations numbers over a two year period differed for each fractions. counts >35 μm fraction mainly dependent on biomass Microcystis colonies. Their peaks observed summer. In fractions, several observed, influenced quality quantity associated with bacteria....
Intercalibration for counting planktonic bacteria by the acridine orange direct count (AODC) method was made among ten investigators of seven laboratories in Japan. The first calibration one freshwater and two seawater samples sent to belonging five laboratories. Each investigator estimated bacterial abundance these AODC according routine procedures each laboratory. For sample estimates differed a factor 2.3 [coefficient variation (CV), 36%], while they varied greatly factors 4.0 11 (CV's,...
Biological products, such as vaccines, blood antitoxins, and antivenoms, are released into the market following a lot release conducted by National Regulatory Authorities or Control Laboratories, even if their manufacturing marketing have been authorized. Independent regulatory authorities is not procedure unique to Japan, but performed worldwide. Previously, Japan carried out mainly laboratory tests, manufacturers' in-house test records were used reference, involved in decision of release....
Vertical distributions of bacterial numbers, cell sizes, and biomass in the water Lake Fryxell McMurdo Dry Valleys Antarctic were examined by acridine orange direct count (AODC) method. Total numbers increased with increasing depth from 5.4×105 cells⋅ml-1 surface (5.0 m depth) to 1.7×107 bottom (19.0 depth). Bacteria waters at all sampling depths dominated rod-shaped cells having average values ranges 0.37-0.64 μm width 1.04-1.85 length. However, those between 10.0 12.5 had relatively large...
ABSTRACT The effect of Bordetella pertussis adenylate cyclase toxin (ACT) on platelet aggregation was investigated. This cell-invasive completely suppressed ADP (10 μM)-induced rabbit platelets at 3 μg/ml and strongly thrombin (0.2 U/ml)-induced 10 μg/ml. suppression accompanied by marked increase in intracellular cyclic AMP (cAMP) content diminished the anti-ACT monoclonal antibody B7E11. A catalytically inactive point mutant ACT did not show suppressive effect. Since an cAMP is a known...
The adenylate cyclase toxin (ACT) of Bordetella pertussis internalizes its catalytic domain into target cells. ACT can function as a tool for delivering foreign protein antigen moieties immune effector cells to induce cytotoxic T lymphocyte response. In this study, we replaced the with an enzymatically active moiety, S1 (ADP-ribosyltransferase) subunit (PT). moiety was successfully internalized independent endocytosis sheep erythrocytes. introduced polypeptide exhibited...
Encystment and excystment in the life cycle of a dinoflagellate Peridinium sp. B3, which was isolated from Lake Begnas Pokhara Valley, Nepal, were investigated by use clonal population. The maximum cell concentration 3×103 vegetative cells·ml-1 BL medium after about 4 weeks at 30°C. Sexual reproduction cells induced late logarithmic phase inoculated into nitrogen phosphorous deficient medium, also spontaneously medium.Sexual isogamous homothallic. initiated gamete formation, gametes produced...