A5088328263- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Infectious Diseases and Mycology
- Biosensors and Analytical Detection
- Infectious Diseases and Tuberculosis
- Antibiotic Resistance in Bacteria
- Diagnosis and treatment of tuberculosis
- Otolaryngology and Infectious Diseases
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Pneumonia and Respiratory Infections
- RNA and protein synthesis mechanisms
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced biosensing and bioanalysis techniques
- Plant Pathogenic Bacteria Studies
- Neonatal and Maternal Infections
- Mosquito-borne diseases and control
- RNA modifications and cancer
- Cytomegalovirus and herpesvirus research
- Glycosylation and Glycoproteins Research
- Cancer therapeutics and mechanisms
- Biochemical and Molecular Research
- Microbial infections and disease research
Osaka Prefectural Institute of Public Health
2010-2024
Osaka City University
2012
Czech Academy of Sciences, Institute of Microbiology
1997
Streptomycin has been an important drug for the treatment of tuberculosis since its discovery in 1944. But numerous strains Mycobacterium tuberculosis, bacterial pathogen that causes are now streptomycin resistant. Although such resistance is often mediated by mutations within rrs, a 16S rRNA gene or rpsL, which encodes ribosomal protein S12, these found limited proportion clinically isolated streptomycin-resistant M. strains. Here we have succeeded identifying mutation confers low-level to...
ABSTRACT In Mycobacterium smegmatis and a limited number of tuberculosis strains, the involvement alterations 16S rRNA gene ( rrs ) in resistance to kanamycin has been shown. To investigate extent which mutations specific region kanamycin-resistant phenotype clinically isolated M. strains were correlated, 43 (MICs, ≧200 μg/ml), 71 kanamycin-susceptible 4 type examined. The 300-bp DNA fragments carrying intervening sequence between 23S rrl amplified by PCR subjected PCR-based direct...
The distribution of Mycobacterium avium-intracellulare complex (MAC) in residences was examined. MAC only recovered from bathrooms but not other sites residences. appearance ratio the patients with pulmonary significantly higher than that healthy volunteers' (P = .01). For 2 patients, genotypes environmental isolates were identical to their respective clinical isolates.
A number of nucleic acid amplification assays (NAAs) have been employed to detect tubercle bacilli in clinical specimens for tuberculosis (TB) diagnosis. Among these, loop-mediated isothermal (LAMP) is an NAA possessing superior reaction characteristics. In the present study, a set six specific primers targeting Mycobacterium 16S rRNA gene with high sensitivity was selected and LAMP system (MTB-LAMP) developed. Using this system, total 200 sputum samples from Nepalese patients were...
ABSTRACT In Japan, more than 10% of streptococcal toxic shock-like syndrome (TSLS) cases have been caused by Streptococcus pyogenes M3/T3 isolates since the first reported TSLS case in 1992. Most from or severe invasive infection during 1992 to 2001 and those noninvasive this period are indistinguishable pulsed-field gel electropherograms. The longest fragments these recent were 300 kb size, whereas recovered before 1973 260 size. These 260- 300-kb hybridized each other, suggesting...
ABSTRACT Mutations in particular nucleotides of genes coding for drug targets or drug-converting enzymes lead to resistance Mycobacterium tuberculosis . For rapid detection drug-resistant M. clinical specimens, a simple and applicable method is needed. Eight TaqMan minor groove binder (MGB) probes, which discriminate one-base mismatches, were designed (dual-probe assay with four reaction tubes). The target six MGB probes was the rpoB gene, involved rifampin resistance; five detect mutation...
The number of patients with severe invasive group-G streptococcal ( Streptococcus dysgalactiae subsp. equisimilis ) infections has been increasing in Japan. emm genotypes and Sma I-digested pulsed-field gel electrophoresis DNA profiles were variable among the strains isolated, suggesting there not clonal expansion a specific subpopulation strains. However, all carried scpA , ska slo sag genes, some which may be involved pathogenesis disease.
Abstract Background Rapid diagnosis of Mycobacterium tuberculosis ( Mtb ) is key to controlling the spread tuberculosis, which a global health concern. In this study, isothermal recombinase polymerase amplification RPA was developed detect specific targets , IS 6110 and 1081. Additionally, SYBR Green I used for endpoint detection products by naked eye. Method A total 146 genomic DNA samples 24 nontuberculous mycobacteria NTM were amplified at 1081 . After complete amplification, amplicons...
Abstract Background Species identification of isolates belonging to the Mycobacterium tuberculosis complex (MTC) seems be important for appropriate treatment patients, since M. bovis is naturally resistant a first line anti-tuberculosis (TB) drug, pyrazinamide, while most other MTC members are susceptible this antimicrobial agent. A simple and low-cost differentiation method was needed in higher TB burden countries, such as Bangladesh, where prevalence among people or cattle has not been...
Abstract Mycobacterium tuberculosis ( Mtb ) strains of Beijing lineage have caused great concern because their rapid emergence drug resistance and worldwide spread. DNA mutation rates that reflect evolutional adaptation to host responses the appearance not been elucidated in human-infected strains. We tracked obtained an original isolate from 1999 outbreak Japan, as well five other isolates spread humans, two patient recurrence. Three were patients who developed TB within one year after...
Multidrug-resistant tuberculosis (MDR-TB) poses a serious threat to TB control. Early diagnosis and proper treatment are essential factors limit the spread of disease. The existing molecular tests for MDR-TB usually require specific instruments, steady power supply, routine maintenance, which might be obstacles low-resource settings. This study aimed develop allele-specific isothermal recombinase polymerase amplification (allele-specific RPA) simultaneously detect most common mutations in...
A rapid, simple, and low-cost diagnostic tool for tuberculosis (TB) detection is urgently needed in countries with a high TB burden. Here, we report novel loop-mediated isothermal amplification (LAMP) assay targeting the hspX gene rapid of Mycobacterium tuberculosis, M. bovis, africanum, microti. The specificity this was evaluated using 4 reference strains complex (MTC), 22 species non-tuberculous mycobacteria (NTM), 7 non-mycobacterial species, 50 clinical isolates. All MTC isolates were...
ABSTRACT Pyrazinamide (PZA), an analog of nicotinamide, is a prodrug for tuberculosis which requires conversion to the bactericidal compound pyrazinoic acid by bacterial pyrazinamidase activity. Mutations leading loss activity cause PZA resistance in Mycobacterium . Thus, detection makes discrimination PZA-resistant possible. However, M. isolates needs large amount bacilli and therefore time consuming. In this paper, we describe new method with PCR-based system. The genes encoding ( pncA...
In spite of the importance hyaluronan in host protection against infectious organisms alveolar spaces, its role mycobacterial infection is unknown. a previous study, we found that mycobacteria interact with on lung epithelial cells. Here, have analyzed after was established and pathogenic can grow by utilizing as carbon source. Both mouse human possess 3 kinds synthases (HAS), designated HAS1, HAS2, HAS3. Utilizing individual HAS-transfected cells, show HAS1 HAS3 but not HAS2 support growth...
The purpose of this study was to examine characteristic profiles Streptococcus pyogenes clinical isolates isolated in Japan during 1994–9. Genotyping the M protein ( emm typing) revealed that types 12 and 28 were most common among 316 isolates. Most emm12 from mucosa, while emm58 emm89 skin. Moreover, emm3 dominant invasive infections. distribution 6 superantigen genes showed all harboured mf gene many had spe G gene. Invasive shown have ssa at a higher rate (76%) than noninvasive (37%)....
Bovine tuberculosis (TB) caused by Mycobacterium bovis is a significant health threat to cattle and zoonotic for humans in many developing countries. Rapid accurate detection of M . fundamental controlling the disease animals humans, proper treatment patients as one first-line anti-TB drug, pyrazinamide, ineffective against Currently, there are no rapid, simplified low-cost diagnostic methods that can be easily integrated use Here, we report development loop-mediated isothermal amplification...
Journal Article Characterization of the rpsL and rrs genes streptomycin‐resistant clinical isolates Mycobacterium tuberculosis in Japan Get access C. Katsukawa, Katsukawa Laboratory Microbiology Dr Chihiro Microbiology, Osaka Prefectural Institute Public Health, 1‐3‐69, Nakamichi, Higashinari‐ku, Osaka, 537, (e‐mail : katsukawa@iph.pref.osaka.jp) Search for other works by this author on: Oxford Academic Google Scholar A. Tamaru, Tamaru Y. Miyata, Miyata Abe, Abe Research Tuberculosis,...
ABSTRACT The Beijing genotype of Mycobacterium tuberculosis is known to be a worldwide epidemic clade. It suggested possibly resistant clone against BCG vaccination and also highly pathogenic prone becoming drug resistant. Thus, monitoring the prevalence this lineage seems important for proper control tuberculosis. Rv0679c protein M. has been predicted one outer membrane proteins contribute host cell invasion. Here, we conducted sequence analysis gene using clinical isolates found that...
We surveyed T serotypes and emm genotypes of Streptococcus pyogenes isolates from streptococcal toxic shock-like syndrome (TSLS) patients. T1 (emm1) remained dominant through 1992 to 2000, but the T3 (emm3.1) strains 1995 disappeared during 1996-2000. Strains several emerged 1996-2000, indicating alterations in prevalent causing TSLS.
To clarify the relationship between epidemics of severe invasive group A streptococcal infections (streptococcal Toxic Shock-Like Syndrome; TSLS) and common in Japan, we examined T serotypes S. pyogenes strains (group streptococci) isolated from clinical specimens (17999 cases) period 1990–5, including (TSLS) (29 1992–5. Characteristic points analyses were: (1) dominant these periods were T12, T4, T1, T28 TB3264, which consistently isolated; (2) isolates T3 rapidly increased through 1990 to...
Tuberculosis (TB) is a serious infectious disease caused by bacterial pathogen. Mortality from tuberculosis was estimated at 1.5 million deaths worldwide in 2013. Development of new TB drugs needed to not only shorten the medication period but also treat multi-drug resistant and extensively drug-resistant TB. Mycobacterium (Mtb) grows slowly multiplies once or twice per day. Therefore, conventional drug screening takes more than 3 weeks. Additionally, biosafety level-3 (BSL-3) facility...