A5017746637- Mycobacterium research and diagnosis
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Tuberculosis Research and Epidemiology
- Legionella and Acanthamoeba research
- Reproductive tract infections research
- Gut microbiota and health
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Pneumonia and Respiratory Infections
- Streptococcal Infections and Treatments
- Aquaculture disease management and microbiota
- Antimicrobial Resistance in Staphylococcus
- Clostridium difficile and Clostridium perfringens research
- Bacterial biofilms and quorum sensing
- Microbial Community Ecology and Physiology
- Maternal Mental Health During Pregnancy and Postpartum
- Genomics and Phylogenetic Studies
- Otolaryngology and Infectious Diseases
- Urinary Tract Infections Management
- Nosocomial Infections in ICU
- Maternal and Perinatal Health Interventions
- Respiratory viral infections research
- Pleural and Pulmonary Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Fukuoka Jo Gakuin Nursing University
2022
University of Occupational and Environmental Health Japan
2011-2021
Fukuoka Jo Gakuin University
2021
Kyushu University
1977-2020
Department of Medical Sciences
2020
Korea Research Institute of Chemical Technology
2016
Kyushu Institute of Technology
2012
Kyoto University
2009-2012
Samsung Medical Center
2011
Sungkyunkwan University
2011
Background Molecular biological modalities with better detection rates have been applied to identify the bacteria causing infectious diseases. Approximately 10–48% of bacterial pathogens community-acquired pneumonia are not identified using conventional cultivation methods. This study evaluated bacteriological causes a cultivation-independent clone library analysis 16S ribosomal RNA gene bronchoalveolar lavage specimens, and compared results those Methods Patients were enrolled based on...
ABSTRACT In Mycobacterium smegmatis and a limited number of tuberculosis strains, the involvement alterations 16S rRNA gene ( rrs ) in resistance to kanamycin has been shown. To investigate extent which mutations specific region kanamycin-resistant phenotype clinically isolated M. strains were correlated, 43 (MICs, ≧200 μg/ml), 71 kanamycin-susceptible 4 type examined. The 300-bp DNA fragments carrying intervening sequence between 23S rrl amplified by PCR subjected PCR-based direct...
Using 39 clinical isolates of Mycobacterium tuberculosis strains with a broad range susceptibility to rifampicin, we examined the relationship between degree resistance rifampicin and mutational sites rpoB gene. All rifampicin-resistant had missense mutations. Twenty (95%) mutation in cluster I region, which has also been reported Escherichia coli [Jin Gross (1988) J. Mol. Biol. 202, 45–58], remaining one strain at codon 381 [Ala → Val] N-terminal not E. coli. Among 18 rifampicin-susceptible...
Two hemolysin genes of Vibrio parahaemolyticus WP1, a thermostable direct (TSD) gene and thermolabile gene, were cloned into the pBR322 vector in Escherichia coli K-12 C600. A large amount TSD produced E. accumulated periplasmic space. The was localized on 0.9-kilobase HindIII-BamHI fragment by identifying qualitatively production reverse passive hemagglutination assay osmotic shock fluid. isolated 1.3-kilobase HindIII-PstI selection with blood agar. Southern blot hybridization colony...
Aspiration pneumonia has been a growing interest in an aging population. Anaerobes are important pathogens, however, the etiology of aspiration is not fully understood. In addition, relationship between patient clinical characteristics and causative pathogens patients with risk factors unclear. To evaluate for bacterial flora bronchoalveolar lavage fluid (BALF) patients, floral analysis 16S ribosomal RNA gene was applied addition to cultivation methods BALF samples. From April 2010 February...
Lecithin-dependent haemolysin (LDH) of Vibrio parahemolyticus was purified from Escherichia coli C600 transformed with a plasmid (pHL591) ligated 1·5 kb DNA fragment V. parahaemolyticus. The final preparation comprised two LDH proteins different molecular masses which were immunologically crossreactive and had the same enzymic activity. phospholipase hydrolysing both fatty acid esters phospholipid, i.e. it hydrolysed phosphatidylcholine (PC) to lysophosphatidylcholine (LPC) then LPC...
We examined the molecular mechanisms of resistance to kanamycin and viomycin in Mycobacterium smegmatis. All M. smegmatis strains with high-level had a nucleotide substitution from A G at position 1389 16S rRNA gene (rrs). This is equivalent 1408 Escherichia coli, mutation this known cause aminoglycoside resistance. Mutations or T 1473 rrs were found viomycin-resistant mutants which been designated vicB our earlier studies. Using conjugation system, we confirmed that these mutations indeed...
In previous studies, neutrophil-ingesting macrophages were clearly and easily observed in the peritoneal cavity of guinea pigs after intraperitoneal injection thioglycolate medium, phagocytosis neutrophils by could be detected vitro cultures exudate cells. Using an system, we examined effect bacterial lipopolysaccharide recombinant human granulocyte colony-stimulating factor on apoptosis (programmed cell death) their subsequent ingestion macrophages. Lipopolysaccharide delayed karyopyknosis...
Physician Job Satisfaction and Working Conditions in Japan: Koji W ada , et al . Department of Preventive Medicine Public Health, Kitasato University School Objectives The aim this study was to determine factors working conditions associated with job satisfaction among physicians Japan. Methods We sent a questionnaire all the who graduated from medical school Physicians were satisfied their determined as those selected “very satisfied” “satisfied” response question: “Overall, are you your...
The incidence of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infection has been increasing; however, the sources remain unclear. Therefore, we investigated involvement meat as a possible mediator CA-MRSA infection. We examined distribution MRSA strains in commercially distributed raw samples (n = 197) and diarrheal stool outpatients 1,287) that were collected Oita Prefecture, Japan, between 2003 2009 for routine legal inspections. Fourteen isolated from three 11...
Background The causative pathogens of healthcare-associated pneumonia (HCAP) remain controversial, and the use conventional cultivation sputum samples is occasionally inappropriate due to potential for oral bacterial contamination. It also sometimes difficult determine whether methicillin-resistant Staphylococcus aureus (MRSA) a true pathogen HCAP. Methods We evaluated diversity in bronchoalveolar lavage fluid (BALF) using molecular methods 82 HCAP patients. BALF specimens were obtained from...
We need look no further than midwifery for compelling evidence of gender inequalities blocking progress in global health. Despite growing the extensive impact midwifery,1-6 midwives and women they care are disempowered by patriarchal structures professional, socio-cultural, economic barriers.7, 8 Widespread misunderstanding role scope exists at all levels policy, health services, academia, funders.9 The consequence is fragmentation care, with inevitable safety quality gaps.8 This retards on...
An Epidemiological Study of Work‐related Violence Experienced by Physicians who Graduated from a Medical School in Japan: Mayuri A rimatsu , et al . Department Work Systems and Health, Institute Industrial Ecological Sciences, University Occupational Environmental Japan —Healthcare workers are at high risk being victim verbal physical violence perpetrated patients. There only few studies on work related among physicians. The aim this study was to determine how prevalent work‐related against...
<b><i>Background:</i></b> Appropriate antibiotic use reduces the mortality of patients with lung abscess; however, 40-60% bacterial etiologies in these have remained unknown culture methods. Obligate anaerobes and <i>Streptococcus anginosus</i> group are common pathogens abscess, but a precise evaluation bacteria by ordinary methods seems to be difficult due upper respiratory tract contamination. <b><i>Objectives:</i></b> The aim...
Background Accumulating evidence has shown that methionine- and choline-deficient high fat (MCD+HF) diet induces the development of nonalcoholic fatty liver disease (NAFLD), in which elevated reactive oxygen species play a crucial role. We have reported peroxiredoxin 4 (PRDX4), unique secretory member PRDX antioxidant family, protects against NAFLD progression. However, detailed mechanism potential effects on intestinal function still remain unclear. Methods & Results Two weeks after feeding...
An influenza epidemic is still a problem despite the development of vaccines and anti-influenza drugs. Preventive measures such as handwashing are fundamental important for counteracting virus infection. In this study, we clarified effects surfactants, which main components hand soaps washing: potassium oleate (C18:1), sodium laureth sulfate (LES) lauryl (SDS). For human strain (H3N2), C18:1 reduced infectivity by 4 logs or more, whereas LES SDS 1 log less. Similar results were obtained when...
We have found that Legionella dumoffii strain Tex-KL (ATCC 33343) invades into and proliferates in the human lung alveolar epithelial-cell line A549 vitro. The organism associated with cells at a 10-fold greater magnitude than L. pneumophila Philadelphia-1 during vitro coculture for 1 h. Thereafter, invaded significantly higher rate (100- to 1,000-fold) did Philadelphia-1. After internalization, however, both bacteria proliferated same rate. This finding led us examine bacterial localization...
After treatment with mitomycin D and other antibacterial agents, a translucent, smooth-colony-forming mycobacterium, isolated from sputum designated as Mycobacterium intracellulare strain 103, gave rise to variants forming opaque colonies. These were more sensitive streptomycin, kanamycin, viomycin, rifampin than the wild-type translucent variants. Plasmid deoxyribonucleic acids taken cells of certain analyzed by agarose gel electrophoresis. Two plasmids molecular weights approximately 2 x...
Journal Article A revised biosynthetic pathway for phosphatidylinositol in Mycobacteria Get access Hiroyuki Morii, Morii * 1Department of Chemistry and 2Department Microbiology, University Occupational Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555, Japan *Hiroyuki Department Chemistry, Japan. Tel: +81 93 603 1611, Fax: 693 9921, email: h-morii@health.uoeh-u.ac.jp Search other works by this author on: Oxford Academic PubMed Google Scholar Midori Ogawa, Ogawa...