A5071520231- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cardiovascular and exercise physiology
- Quinazolinone synthesis and applications
- Bacteriophages and microbial interactions
- Respiratory Support and Mechanisms
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Immunodeficiency and Autoimmune Disorders
- Diagnosis and treatment of tuberculosis
- Immune responses and vaccinations
- Genomics and Phylogenetic Studies
- Microbial Natural Products and Biosynthesis
- RNA and protein synthesis mechanisms
- Tannin, Tannase and Anticancer Activities
- Infectious Diseases and Tuberculosis
- Regulation of Appetite and Obesity
- Cancer therapeutics and mechanisms
- Pharmacological Effects of Natural Compounds
- Inhalation and Respiratory Drug Delivery
- Bacterial Genetics and Biotechnology
- Respiratory and Cough-Related Research
- Heart Rate Variability and Autonomic Control
- Glycosylation and Glycoproteins Research
Niigata University
2014-2024
Fujita Health University
2024
Airlangga University
2024
University of Minnesota Medical Center
2024
Japan Anti Tuberculosis Association
2024
Niigata University Medical and Dental Hospital
2017-2023
Toneyama National Hospital
2004-2022
National Hospital Organization
2008-2022
National Institute of Allergy and Infectious Diseases
2021
National Institutes of Health
2021
Background Pulmonary cachexia is common in advanced chronic obstructive pulmonary disease (COPD), culminating exercise intolerance and a poor prognosis. Ghrelin novel growth hormone (GH)-releasing peptide with GH-independent effects. The efficacy safety of adding ghrelin to rehabilitation (PR) cachectic COPD patients were investigated. Methodology/Principal Findings In multicenter, randomized, double-blind, placebo-controlled trial, 33 randomly assigned PR intravenous (2 µg/kg) or placebo...
Although Mycobacterium avium complex pulmonary disease (MAC-PD) is a growing health problem, little known about long-term radiographic outcome and factors for deterioration in patients with MAC-PD.Data on nodular bronchiectatic (NBE) MAC-PD who underwent regular follow-up >5 years were retrospectively reviewed. Changes plain chest radiograph (CXR) baseline characteristics compared between the stable deteriorated groups.Seventy-two investigated, including 30 examined 10 after initial visit....
Tuberculosis remains one of the most deadly infectious diseases worldwide and is a leading public health problem. Although isoniazid (INH) key drug for treatment tuberculosis, tolerance to INH necessitates prolonged treatment, which concern effective tuberculosis chemotherapy. prodrug that activated by mycobacterial enzyme, KatG. Here, we show DNA-binding protein 1 (MDP1), histone-like conserved in mycobacteria, negatively regulates katG transcription leads phenotypic mycobacteria....
Abstract Survival of the live attenuated Bacillus Calmette-Guérin (BCG) vaccine amidst harsh host environments is key for BCG effectiveness as it allows continuous immune response induction and protection against tuberculosis. Mycobacterial DNA binding protein 1 (MDP1), a nucleoid associated protein, essential in BCG. However, there limited knowledge on extent MDP1 gene regulation how this influences survival. Here, we demonstrate that conditional knockdown (cKD) grows slower than vector...
Medical treatment of pulmonary Mycobacterium avium complex (MAC) disease does not always provide curative effects and is frequently hampered by recurrence. This suggests the presence a reservoir for MAC in environment surrounding patients. We previously reported recovery isolates from residential bathrooms outpatients. In present study, to ascertain colonizing sites possibility an patients, we tested genetic diversity 6 specimens, including 2 additional sampling sites, inside showerhead...
Abstract Mycobacterium avium subsp. hominissuis (MAH) is the major causative agent of nontuberculous mycobacteriosis, representative case environment-related infectious diseases incidence which increasing in industrialized countries. MAH found biofilm drinking water distribution system and residential environments. We investigated effect gaseous nutritional conditions, role glycopeptidolipids (GPLs) on biofilm-like pellicle formation MAH. Pellicle was observed under 5% oxygen Middlebrook 7H9...
Abstract Mycobacterium tuberculosis ( Mtb ) strains of Beijing lineage have caused great concern because their rapid emergence drug resistance and worldwide spread. DNA mutation rates that reflect evolutional adaptation to host responses the appearance not been elucidated in human-infected strains. We tracked obtained an original isolate from 1999 outbreak Japan, as well five other isolates spread humans, two patient recurrence. Three were patients who developed TB within one year after...
Abstract Background Mycobacterium intracellulare is a major etiological agent of avium-intracellulare pulmonary disease (MAC-PD). However, the characteristics virulence M. and in vivo chemotherapeutic efficacy remain unclear. In this study, we examined nine strains with different clinical phenotypes genotypes C57BL/6 mice. Results We classified three types (high, intermediate, low) based on kinetics bacterial load, histological lung inflammation, neutrophilic infiltration. High showed more...
Bacteria can proliferate perpetually without ageing, but they also face conditions where must persist. Mycobacteria survive for a long period. This state appears during mycobacterial diseases such as tuberculosis and leprosy, which are chronic develop after long-term persistent infections. However, the fundamental mechanisms of living mycobacteria unknown. Every Mycobacterium species expresses Mycobacterial DNA-binding protein 1 (MDP1), histone-like nucleoid associated protein. smegmatis is...
One-third of the world's humans has latent tuberculosis infection (LTBI), representing a large pool potentially active TB. Recent LTBI carries higher risk disease progression than remote LTBI. studies suggest important roles antibodies in TB pathology, prompting us to investigate serum antibody profiles cohort with In this single-center prospective observational study, we analyzed IgG-antibody concentrations against five major Mycobacterium (Mtb) antigens (including 6 kDa early secretory...
Abstract Background Mycobacterium intracellulare is a representative etiological agent of emerging pulmonary M. avium-intracellulare complex disease in the industrialized countries worldwide. The recent genome sequencing clinical strains isolated from has provided insight into genomic characteristics pathogenic mycobacteria, especially for avium ; however, remain to be elucidated. Results In this study, we performed comparative analysis 55 and related such as paraintracellulare (MP), indicus...
The global incidence of the human nontuberculous mycobacteria (NTM) disease is rapidly increasing. However, knowledge gene essentiality under optimal growth conditions and relevant to natural ecology NTM, such as hypoxia, lacking. In this study, we utilized transposon sequencing comprehensively identify genes essential for in Mycobacterium intracellulare. Of 5126 M. intracellulare ATCC13950, 506 were identified genes, which 280 158 shared with tuberculosis marinum, respectively. included...
The survival rate of chronic obstructive pulmonary disease (COPD) patients with severely reduced exercise capacity is extremely low. We recently identified three life-threatening pathophysiological conditions during cardiopulmonary testing (CPET): (1) exercise-induced hypoxemia, (2) sympathetic overactivity, and (3) progressive respiratory acidosis at low-intensity exercise. present prospective observation study aimed to determine whether these parameters constitute risk factors mortality in...
The aim of this substudy the ghrelin treatment, multicenter, randomized, double-blind, placebo-controlled trial was to investigate effects administration on exercise capacity and underlying mechanisms in underweight patients with chronic obstructive pulmonary disease (COPD) using cardiopulmonary testing. Twenty COPD were randomized rehabilitation intravenous (2 μg/kg, n = 10) or placebo (n twice daily for 3 weeks a double-blind fashion. primary outcome changes peak oxygen uptake . Secondary...
<h3>BACKGROUND:</h3> Patients with COPD have reduced exercise tolerance associated dyspnea. This intolerance is primarily due to impaired ventilatory mechanics, but it also a combination of factors, including inefficient gas exchange, lactic acidosis at low work rate, and exercise-induced hypoxemia. The survival prognosis patients severely capacity extremely poor, the pathophysiology these during remains be accurately established. present study aimed characterize life-threatening factors...
Exertional dyspnoea limits patients with IPF in their activities of daily living. The mechanism, however, has not been elucidated. This study tested the hypothesis that exertional correlates cardiopulmonary exercise responses, specifically changes arterial blood pH and plasma norepinephrine (NE).Cardiopulmonary testing measurements (Borg scale), NE, lactate gases were performed 29 nine controls.Both groups showed obvious break points during exercise. In IPF, an abrupt change Borg scale, pH,...
The results of studies on the oxygen response in patients with COPD should provide important clues to pathophysiology exertional dyspnoea. We investigated exercise responses hyperoxia relation dyspnoea profile, as well cardiopulmonary, acidotic and sympathetic parameters 35 stable (mean FEV(1) 46% predicted).This was a single-blind trial, which breathed 24% O(2) or compressed air (CA) random order during two incremental cycle tests.PaO(2) PaCO(2) were higher (P < 0.0001 P 0.05, respectively)...
Abstract The architecture of the genome influences functions DNA from bacteria to eukaryotes. Intrinsically disordered regions (IDR) eukaryotic histones have pivotal roles in various processes gene expression. IDR is rare bacteria, but interestingly, mycobacteria produce a unique histone-like protein, MDP1 that contains long C-terminal IDR. Here we analyzed role function. By employing Mycobacterium smegmatis inducibly expresses or its IDR-deficient mutant, observed induces IDR-dependent...
Delamanid (DLM), a nitro-dihydroimidazooxazole derivative currently approved for pulmonary multidrug-resistant tuberculosis (TB) therapy, is prodrug activated by mycobacterial 7,8-didemethyl-8-hydroxy 5-deazaflavin electron transfer coenzyme (F 420 )-dependent nitroreductase (Ddn). Despite inhibiting the biosynthesis of subclass mycolic acids, active DLM metabolite remained unknown. Comparative liquid chromatography-mass spectrometry (LC-MS) analysis metabolites revealed covalent binding...
Development of accurate methods for predicting progression tuberculosis (TB) from the latent state is recognized as vitally important in controlling TB, because a majority cases develop infections. Past TB that has never been treated higher risk progressing than does Mycobacterium infection patients who have previously received treatment. Antibody responses against 23 kinds M. proteins individuals with past had not medicated were evaluated. These significantly concentrations antibodies...
Pyrazolo[1,5-a]pyrimidin-7(4H)-one was identified through high-throughput whole-cell screening as a potential antituberculosis lead. The core of this scaffold has been several times previously and associated with various modes action against Mycobacterium tuberculosis (Mtb). We explored the synthesis focused library analogues key features pharmacophore while achieving substantial improvements in antitubercular activity. Our best hits had low cytotoxicity showed promising activity Mtb within...
Abstract DNA is basically an intracellular molecule that stores genetic information and carries instructions for growth reproduction in all cellular organisms. However, some bacteria, has additional roles outside the cells as extracellular (eDNA), which essential component of biofilm formation hence antibiotic tolerance. Mycobacteria include life-threating human pathogens, most are slow growers. little known about nature pathogenic mycobacteria’s eDNA. Here we found eDNA present slow-growing...