A5063589047- Malaria Research and Control
- Mosquito-borne diseases and control
- Tuberculosis Research and Epidemiology
- Complement system in diseases
- Biochemical and Molecular Research
- Quinazolinone synthesis and applications
- Vector-borne infectious diseases
- Immune Cell Function and Interaction
- Inflammasome and immune disorders
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Impact on Reproduction
- Global Maternal and Child Health
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Mycobacterium research and diagnosis
- Tryptophan and brain disorders
- Trauma and Emergency Care Studies
- Long-Term Effects of COVID-19
- Vaccine Coverage and Hesitancy
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
National Institutes of Health
2020-2025
University of Washington
2021-2025
National Institute of Allergy and Infectious Diseases
2021
Wexham Park Hospital
2014
Post-acute sequelae of COVID-19 (PASC) represent an emerging global crisis. However, quantifiable risk factors for PASC and their biological associations are poorly resolved. We executed a deep multi-omic, longitudinal investigation 309 patients from initial diagnosis to convalescence (2-3 months later), integrated with clinical data patient-reported symptoms. resolved four PASC-anticipating at the time diagnosis: type 2 diabetes, SARS-CoV-2 RNAemia, Epstein-Barr virus viremia, specific...
Background: Prior to infecting red blood cells and causing the clinical manifestations of malaria, hepatotropic parasite Plasmodium falciparum undergoes a complex liver stage. Biological sex-based differences in pathogenesis by microorganisms are well-documented, but unstudied for P. humans. We conducted pooled analysis on data from participants placebo arms infectivity controls Controlled Human Malaria Infection (CHMI) trials malaria-naïve populations evaluate impact biological sex time...
Pyrazolo[1,5-a]pyrimidin-7(4H)-one was identified through high-throughput whole-cell screening as a potential antituberculosis lead. The core of this scaffold has been several times previously and associated with various modes action against Mycobacterium tuberculosis (Mtb). We explored the synthesis focused library analogues key features pharmacophore while achieving substantial improvements in antitubercular activity. Our best hits had low cytotoxicity showed promising activity Mtb within...
Tryptophan biosynthesis represents an important potential drug target for new anti-TB drugs. We identified a series of indole-4-carboxamides with potent antitubercular activity. In vitro, Mycobacterium tuberculosis (Mtb) acquired resistance to these compounds through three discrete mechanisms: (1) decrease in metabolism via loss-of-function mutations the amidase that hydrolyses carboxamides, (2) increased biosynthetic rate tryptophan precursors loss allosteric feedback inhibition...
Abstract Radiation-attenuated sporozoite (RAS) vaccines can completely prevent blood stage Plasmodium infection by inducing liver-resident memory CD8 + T cells to target parasites in the liver. Such be induced ‘Prime-and-trap’ vaccination, which here combines DNA priming against P. yoelii circumsporozoite protein (CSP) with a subsequent intravenous (IV) dose of liver-homing RAS “trap” activated and expanding Prime-and-trap confers durable protection mice, efforts are underway translate this...
Abstract Background Plasmodium knowlesi is an established experimental model for basic and pre-clinical malaria vaccine research. Historically, rhesus macaques have been the most common host studies with P. parasites. However, are not natural hosts , there interest in identifying alternative The study team previously reported that pig-tailed (PTM), a could be challenged cryopreserved sporozoites (PkSPZ), time to blood stage infection equivalent rhesus. Here, additional exploratory were...
Tryptophan biosynthesis represents an important potential drug target for new anti-TB drugs. We identified a series of indole-4-carboxamides with potent antitubercular activity. In vitro, Mycobacterium tuberculosis (Mtb) acquired resistance to these compounds through three discrete mechanisms: (1) decrease in metabolism via loss-of-function mutations the amidase that hydrolyses carboxamides, (2) increased biosynthetic rate tryptophan precursors loss allosteric feedback inhibition...
7DW8-5 is a potent glycolipid adjuvant that improves malaria vaccine efficacy in mice by inducing IFN-γ and increasing protective CD8+ T cell responses. The addition of was previously shown to improve the cell-mediated heterologous 'prime-and-trap' against Plasmodium yoelii sporozoite challenge inbred female mice. Here, we report significant differential sex-specific responses outbred Male express significantly less IL-4 compared females following intravenous administration. Additionally,...
The Collaborative Action in Lowering Maternity Encountered Deaths (CALMED) aims to send vocational training teams (VTTs) help reduce maternal and newborn mortality. This article outlines two midwives' experience during a 2-week VTT programme manage obstetric emergencies India March 2013. CALMED project was organised by Rotary groups London Mumbai.
Abstract Malaria is caused by Plasmodium parasites and was responsible for over 247 million infections 619,000 deaths in 2021. Radiation-attenuated sporozoite (RAS) vaccines can completely prevent blood stage infection inducing protective liver-resident memory CD8 + T cells. Such cells be induced ‘prime-and-trap’ vaccination, which here combines DNA priming against the P. yoelii circumsporozoite protein (CSP) with a subsequent intravenous (IV) dose of liver-homing RAS to “trap” activated...
Abstract Background Plasmodium knowlesi (Pk) is an established experimental model for basic and pre-clinical malaria vaccine research. Historically, rhesus macaques have been the most common host studies with Pk parasites. However, are not natural hosts Pk, there interest in identifying alternative The study team previously reported that pig-tailed (PTM), a could be challenged cryopreserved sporozoites (PkSPZ), time to blood stage infection equivalent rhesus. Here, additional exploratory...
Abstract Generating liver resident-memory CD8+ T cells (TRM cells) is critical for effective liver-stage malaria vaccine. The role of biological sex in vaccine protection understudied. Here we report sex-specific immune responses and outcomes the two-step heterologous ‘Prime-and-Trap’ designed to induce TRM cells. Prime-and-Trap strategy combines sporozoite antigen DNA priming with a single intravenous dose liver-homing radiation-attenuated sporozoites (RAS) direct “trap” activated expanding...