Jongchan Choi
A5102801051- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Single-cell and spatial transcriptomics
- CAR-T cell therapy research
- COVID-19 Clinical Research Studies
- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Microfluidic and Bio-sensing Technologies
- Immune Cell Function and Interaction
- Immune cells in cancer
- Diabetes and associated disorders
- Tryptophan and brain disorders
- Intensive Care Unit Cognitive Disorders
- Biosimilars and Bioanalytical Methods
- Peripheral Neuropathies and Disorders
- Monoclonal and Polyclonal Antibodies Research
- Inflammasome and immune disorders
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Microbial Metabolic Engineering and Bioproduction
- Multiple Sclerosis Research Studies
- Adipose Tissue and Metabolism
Institute for Systems Biology
2019-2025
Systems Biology Institute
2025
InSysBio (Russia)
2019-2023
Gwangju Institute of Science and Technology
2019-2021
Seattle University
2019
Post-acute sequelae of COVID-19 (PASC) represent an emerging global crisis. However, quantifiable risk factors for PASC and their biological associations are poorly resolved. We executed a deep multi-omic, longitudinal investigation 309 patients from initial diagnosis to convalescence (2-3 months later), integrated with clinical data patient-reported symptoms. resolved four PASC-anticipating at the time diagnosis: type 2 diabetes, SARS-CoV-2 RNAemia, Epstein-Barr virus viremia, specific...
We present an integrated analysis of the clinical measurements, immune cells, and plasma multi-omics 139 COVID-19 patients representing all levels disease severity, from serial blood draws collected during first week infection following diagnosis. identify a major shift between mild moderate disease, at which point elevated inflammatory signaling is accompanied by loss specific classes metabolites metabolic processes. Within this stressed environment multiple unusual cell phenotypes emerge...
Infection, autoimmunity, and cancer are principal human health challenges of the 21st century. Often regarded as distinct ends immunological spectrum, recent studies hint at potential overlap between these diseases. For example, inflammation can be pathogenic in infection autoimmunity. T resident memory (TRM) cells beneficial cancer. However, findings limited by size scope; exact factors shared across diseases remain elusive. Here, we integrate large-scale deeply clinically biologically...
Elucidating the relationships between a class I peptide antigen, CD8 T cell receptor (TCR) specific to that and phenotype emerges following antigen stimulation, remains mostly unsolved problem, largely due lack of large data sets can be mined resolve such relationships. Here, we describe Antigen-TCR Pairing Multiomic Analysis T-cells (APMAT), an integrated experimental-computational framework designed for high-throughput capture analysis cells, with paired TCR sequence, single-cell...
Elucidating the relationships between a class I peptide antigen, CD8 T cell receptor (TCR) specific to that and phenotype emerges following antigen stimulation, remains mostly unsolved problem, largely due lack of large data sets can be mined resolve such relationships. Here, we describe Antigen-TCR Pairing Multiomic Analysis T-cells (APMAT), an integrated experimental-computational framework designed for high-throughput capture analysis cells, with paired TCR sequence, single-cell...
Adaptive immunity is based on peptide antigen recognition. Our ability to harness the immune system for therapeutic gain relies discovery of T cell receptor (TCR) genes that selectively target antigens from infections, mutated proteins, and foreign agents. Here we present a method labels antigen-specific CD8+ cells using magnetic nanoparticles functionalized with peptide-MHC tetramers, isolates these specific within an integrated microfluidic device, directly amplifies TCR sequencing....
We report here on antigens from the SARS-CoV-2 virus spike protein, that when presented by Class I MHC, can lead to cytotoxic CD8+ T cell anti-viral responses in COVID-19 patients. present a method which protein is converted into library of peptide antigen-Major Histocompatibility Complexes (pMHCs) as single chain trimers contain antigen, MHC HLA allele, and β-2 microglobulin sub-unit. That used detect evolution virus-specific populations two patients, at time points over course infection....
SUMMARY Host immune responses play central roles in controlling SARS-CoV2 infection, yet remain incompletely characterized and understood. Here, we present a comprehensive response map spanning 454 proteins 847 metabolites plasma integrated with single-cell multi-omic assays of PBMCs which whole transcriptome, 192 surface proteins, T B cell receptor sequence were co-analyzed within the context clinical measures from 50 COVID19 patient samples. Our study reveals novel cellular subpopulations,...
The discovery and characterization of antigen-specific CD8+ T cell clonotypes typically involves the labor-intensive synthesis construction peptide-MHC tetramers. We adapt single-chain trimer (SCT) technologies into a high throughput platform for pMHC library generation, showing that hundreds can be rapidly prepared across multiple Class I HLA alleles. use this to explore impact peptide SCT template mutations on protein expression yield, thermal stability, functionality. libraries were an...
Host immune responses play central roles in controlling SARS-CoV2 infection, yet remain incompletely characterized and understood. Here, we present a comprehensive response map spanning 454 proteins 847 metabolites plasma integrated with single-cell multi-omic assays of 221,748 PBMCs which whole transcriptome, 192 surface proteins, T B cell receptor sequence were analyzed within the context clinical measures from 50 COVID19 patient samples. Our study reveals novel cellular subpopulations,...
<h3>Background</h3> Despite advances with single-clone CD8+ T cells in cancer therapy, results often fall short due to immune evasion by and the limited lifespan of administered cells.<sup>1–3</sup> To address these challenges, there is growing interest utilizing cancer-specific CD4+ cells. However, diversity TCRs Class II HLAs presents challenges identifying antigen-specific across diverse patients. Here, we developed a high-throughput platform screen cells, providing multilayer information...
Abstract The identification of cancer-specific T cell receptor (TCR) sequences is crucial for advancing cancer immunotherapies. Recent studies and clinical trials have revealed a vulnerability in monoclonal therapy, where cells can evade the immune response through loss HLA heterozygosity low antigen heterogeneity. To address this challenge, cocktail therapy using TCRs that target multiple HLAs antigens has been proposed to enhance efficacy adoptive transfer therapy. In pursuit goal, we...
Abstract The immunological picture of how different patients recover from COVID-19, and those recovery trajectories are influenced by infection severity, remain unclear. We investigated 140 COVID-19 diagnosis to convalescence using clinical data, viral load assessments, multi-omic analyses blood plasma circulating immune cells. Immune-phenotype dynamics resolved four trajectories. One trajectory signals a return pre-infection healthy baseline, while the other three characterized differing...
Cancer is a dynamic disease involving constant changes. With these changes, cancer cells become heterogeneous, resulting in varying sensitivity to chemotherapy. The heterogeneity of plays key role chemotherapy resistance and recurrence. Therefore, for effective treatment, need be analyzed at the single-cell level by monitoring various proteins investigating their heterogeneity. We propose microfluidic chip proteomics assay that capable analyzing complex cellular signaling systems reveal...
CD8 + cytotoxic T cell responses against viral infection represent a major element of the adaptive immune response. We describe development peptide antigen - histompatibility complex (pMHC) library representing full SARS-CoV-2 proteome, and comprised 634 pMHC multimers A*02.01, A*24.02, B*07.02 HLA alleles, as well specific antigens associated with cytomegalovirus (CMV). These libraries were used to capture non-expanded cells from blood samples collected 64 infected individuals, then...
Abstract Infection, autoimmunity, and cancer are the principal human health challenges of 21st century major contributors to death disease. Often regarded as distinct ends immunological spectrum, recent studies have hinted there may be more overlap between these diseases than appears. For example, pathogenic inflammation has been demonstrated conserved infection autoimmune settings. T resident memory (T RM ) cells highlighted beneficial for cancer. However, findings limited by patient number...
Abstract Adaptive immunity is based on peptide antigen recognition. Our ability to harness the immune system for therapeutic gain relies discovery of T cell receptor (TCR) genes that selectively target antigens from infections, mutated proteins, and foreign agents. Here we present a method labels antigen-specific CD8+ T-cells in human blood using magnetic nanoparticles functionalized with peptide-MHC tetramers, isolates these specific cells within an integrated microfluidic device, directly...