A5035465005- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Chemical Synthesis and Analysis
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Click Chemistry and Applications
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Diabetes and associated disorders
- Ultrasound and Hyperthermia Applications
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- COVID-19 Clinical Research Studies
- Cancer, Stress, Anesthesia, and Immune Response
California Institute of Technology
2015-2024
McGill University
2022
Institute for Systems Biology
2020
Division of Chemistry
2018
We present an integrated analysis of the clinical measurements, immune cells, and plasma multi-omics 139 COVID-19 patients representing all levels disease severity, from serial blood draws collected during first week infection following diagnosis. identify a major shift between mild moderate disease, at which point elevated inflammatory signaling is accompanied by loss specific classes metabolites metabolic processes. Within this stressed environment multiple unusual cell phenotypes emerge...
Abstract We describe a general synthetic strategy for developing high‐affinity peptide binders against specific epitopes of challenging protein biomarkers. The epitope interest is synthesized as polypeptide, with detection biotin tag and strategically placed azide (or alkyne) presenting amino acid. This (SynEp) incubated library complementary alkyne or peptides. Library elements that bind the SynEp in correct orientation undergo Huisgen cycloaddition, are covalently linked to SynEp. Hit...
ABSTRACT Targeted gene delivery to the brain is a critical tool for neuroscience research and has significant potential treat human disease. However, site-specific of common vectors such as adeno-associated viruses (AAVs) typically performed via invasive injections, limiting their scope clinical applications. Alternatively, focused ultrasound blood-brain-barrier opening (FUS-BBBO), noninvasively, enables entry AAVs into from systemic circulation. when used in conjunction with natural AAV...
Protein catalyzed capture agents (PCCs) are synthetic antibody surrogates that can target a wide variety of biologically relevant proteins. As step toward developing high-throughput PCC pipeline, we report on the preparation barcoded rapid assay platform for analysis hits from library screens. The is constructed by first surface patterning micrometer scale barcode composed orthogonal ssDNA strands onto glass slide. slide then partitioned into microwells, each which contains multiple copies...
Abstract We describe a general synthetic strategy for developing high‐affinity peptide binders against specific epitopes of challenging protein biomarkers. The epitope interest is synthesized as polypeptide, with detection biotin tag and strategically placed azide (or alkyne) presenting amino acid. This (SynEp) incubated library complementary alkyne or peptides. Library elements that bind the SynEp in correct orientation undergo Huisgen cycloaddition, are covalently linked to SynEp. Hit...
Tumor neoantigens are fragments of mutated proteins that contain the mutation, and can be presented by major histocompatibility complex molecules on tumor cells, where they surveyed T cells. The rapid sensitive identification neoantigen‐specific cell populations from tissues or blood has proven challenging. A microchip platform for non‐destructive CD8 cells is described. method utilizes a library neoantigen/MHC tetramers linked to magnetic nanoparticle via DNA barcode. neoantigen‐specificity...
Abstract At the heart of most cancer immunotherapies are specific interactions between principal cell killers, T cells, and antigens presented by tumor cells. Those may be exposed through use checkpoint inhibitors, or they can amplified engineered cell-based therapies. A class that has emerged as being particularly important neoantigens, which small fragments mutated proteins contain mutation, and, for CD8+ recognition, MHC Class I. In principle, neoantigens draw cells into a comprise...
Abstract At the heart of most cancer immunotherapies are specific interactions between tumor-infiltrating T cells, and antigens that attract those cells into tumor. It has become increasingly apparent (mutation containing) neoantigens likely play an important role in this regard. Putative identified by tumor exome analysis, but finding which candidates actually promote cell infiltration is challenging, as pairing such with cognate receptor (TCR) genes. Technologies can address challenge help...
Abstract The tumor microenvironment (TME) is a complex arms race composed of host stroma and rapidly adapting cancer cells. This symbiosis further complicated when dissecting the influence inflammation, due to its multifaceted function within TME as driver both pro- anti-tumor responses. Comprising major component TME, targeting or exploiting tumor-associated inflammation has long been sought for therapeutic purposes. However, optimal amount composition such purposes remains elusive. We have...