A5013465269- Microfluidic and Capillary Electrophoresis Applications
- Electrowetting and Microfluidic Technologies
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biosensors and Analytical Detection
- Nanofabrication and Lithography Techniques
- Advanced Biosensing Techniques and Applications
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Analytical Chemistry and Sensors
- Cancer Immunotherapy and Biomarkers
- Advanced biosensing and bioanalysis techniques
- Molecular Junctions and Nanostructures
- COVID-19 Clinical Research Studies
- Epigenetics and DNA Methylation
- Melanoma and MAPK Pathways
- Mechanical and Optical Resonators
- Advanced Electron Microscopy Techniques and Applications
- Photonic and Optical Devices
- Near-Field Optical Microscopy
- MicroRNA in disease regulation
University of Utah
2003-2024
Institute for Systems Biology
2019-2023
California Institute of Technology
2019-2023
InSysBio (Russia)
2019
Division of Chemistry
2019
Pasadena City College
2019
University of Toronto
2009-2018
Toronto Public Health
2016
Occupational Cancer Research Centre
2014-2015
AbbVie (United States)
2013
Abstract T cell receptors (TCRs) enable cells to specifically recognize mutations in cancer 1–3 . Here we developed a clinical-grade approach based on CRISPR–Cas9 non-viral precision genome-editing simultaneously knockout the two endogenous TCR genes TRAC (which encodes TCRα) and TRBC TCRβ). We also inserted into locus chains of neoantigen-specific (neoTCR) isolated from circulating patients. The neoTCRs were using personalized library soluble predicted neoantigen–HLA capture reagents....
A monolithic waveguide system using poly(dimethyl siloxane) (PDMS) was designed, fabricated, and characterized. The demonstrated good confinement of light relatively low attenuation at 0.40 dB/cm. robustness handling properties the completed waveguides were excellent, process yield exceeded 96%. did exhibit moderate temperature humidity sensitivity but no temporal variation, insertion loss remained stable over extended periods time. Applications this in microscale sensing are immense,...
We introduce a new format for particle-based immunoassays relying on digital microfluidics (DMF) and magnetic forces to separate resuspend antibody-coated paramagnetic particles. In DMF, fluids are electrostatically controlled as discrete droplets (picoliters microliters) an array of insulated electrodes. By applying appropriate sequences potentials these electrodes, multiple can be manipulated simultaneously various droplet operations achieved using the same device design. This flexibility...
Portable digital microfluidic serological immunoassays for measles and rubella were developed evaluated in a remote setting.
We introduce an automated digital microfluidic (DMF) platform capable of performing immunoassays from sample to analysis with minimal manual intervention. This features (a) a 90 Pogo pin interface for control, (b) integrated (and motorized) photomultiplier tube chemiluminescent detection, and (c) magnetic lens assembly which focuses fields into narrow region on the surface DMF device, facilitating up eight simultaneous separations. The new was used implement three-level full factorial design...
The first example of so-called "digital microfluidics" (DMF) implemented on paper by inkjet printing is reported. A sandwich enzyme-linked immunosorbent assay (ELISA) demonstrated as an a complex, multistep protocol that would be difficult to achieve with capillary-driven microfluidics. Furthermore, it shown paper-based DMF devices have comparable performance traditional photolithographically patterned at fraction the cost.
Non-invasively probing metabolites within single live cells is highly desired but challenging. Here we utilize Raman spectro-microscopy for spatial mapping of cells, with the specific goal identifying druggable metabolic susceptibilities from a series patient-derived melanoma cell lines. Each line represents different characteristic level cancer de-differentiation. First, spectroscopy, followed by stimulated scattering (SRS) microscopy and transcriptomics analysis, identify fatty acid...
Digital microfluidics (DMF) has emerged as a popular format for implementing quantitative immunoassays diagnostic biomarkers. All previous reports of such assays have relied on optical detection; here, we introduce the first digital microfluidic immunoassay relying electrochemical detection. In this system, an indium tin oxide (ITO) based DMF top plate was modified to include gold sensing electrodes and silver counter/pseudoreference suitable in-line amperometric measurements. A thyroid...
We report a new technique called Digital microfluidic Immunocytochemistry in Single Cells (DISC). DISC automates protocols for cell culture, stimulation and immunocytochemistry, enabling the interrogation of protein phosphorylation on pulsing with stimulus as little 3 s. was used to probe states platelet-derived growth factor receptor (PDGFR) downstream signalling protein, Akt, evaluate concentration- time-dependent effects stimulation. The high time resolution allowed surprising...
Inkjet printing is combined with roll-coating to fabricate digital microfluidic (DMF) devices outside of the cleanroom for inexpensive, miniaturized diagnostic assays, straightforward scalability towards mass production.
Digital synthesis has been applied to the formation of peptide-based macrocycles and their analogues with side chains appended during late-stage aziridine ring-opening. Discrete nanoliter- microliter-sized droplets samples reagents are controlled in parallel by applying a series electrical potentials an array electrodes coated hydrophobic insulator.
This paper reports the characterization of a microfluidic packaging technique involving use press-fit interconnects to channels molded in PDMS. is implemented by, first, coring small hole PDMS access or buried microchannels using modified 20 gauge needle; and second, inserting an unmodified needle into create direct connection microchannel that requires no bonding molding. The needles can then easily be removed reinserted multiple times since seal created purely by compression around needle....
Abstract BACKGROUND Whereas disease surveillance for infectious diseases such as rubella is important, it critical to identify pregnant women at risk of passing their offspring, which can be fatal and result in congenital syndrome (CRS). The traditional centralized model diagnosing cost-prohibitive resource-limited settings, representing a major obstacle the prevention CRS. As step toward decentralized diagnostic systems, we developed proof-of-concept digital microfluidic (DMF) platform that...
Phenotypic plasticity is associated with non-genetic drug tolerance in several cancers. Such can arise from chromatin remodeling, transcriptomic reprogramming, and/or protein signaling rewiring, and characterized as a cell state transition response to molecular or physical perturbations. This, turn, confound interpretations of responses resistance development. Using BRAF-mutant melanoma lines the prototype, we report on joint theoretical experimental investigation cell-state dynamics BRAF...
A relatively low-cost fabrication method using soft lithography and molding for large-area, high-aspect-ratio microfluidic devices, which have traditionally been difficult to fabricate, has developed is presented in this work. The process includes novel but simple modifications of conventional microfabrication steps can be performed any standard facility. Specifically, the testing a device continuous flow deposition bio-molecules an array format are presented. layout requires elastomeric...
Many important biomarkers for disease diagnosis are present at low concentrations in human serum. These masked proteomic analysis by highly abundant proteins such as serum albumin (HSA) and immunoglobulins (IgGs) which account up to 80% of the total protein content Traditional depletion methods using macro-scale LC-columns involve slow separations impart considerable dilution samples. Furthermore, most techniques lack ability process multiple samples simultaneously. We a method...
Adaptive immunity is based on peptide antigen recognition. Our ability to harness the immune system for therapeutic gain relies discovery of T cell receptor (TCR) genes that selectively target antigens from infections, mutated proteins, and foreign agents. Here we present a method labels antigen-specific CD8+ cells using magnetic nanoparticles functionalized with peptide-MHC tetramers, isolates these specific within an integrated microfluidic device, directly amplifies TCR sequencing....
We report here on antigens from the SARS-CoV-2 virus spike protein, that when presented by Class I MHC, can lead to cytotoxic CD8+ T cell anti-viral responses in COVID-19 patients. present a method which protein is converted into library of peptide antigen-Major Histocompatibility Complexes (pMHCs) as single chain trimers contain antigen, MHC HLA allele, and β-2 microglobulin sub-unit. That used detect evolution virus-specific populations two patients, at time points over course infection....