A5083074463- Electrowetting and Microfluidic Technologies
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Cellular transport and secretion
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biosensors and Analytical Detection
- Mesenchymal stem cell research
- Retinal Development and Disorders
- Bone Tissue Engineering Materials
- Microfluidic and Capillary Electrophoresis Applications
- Protein Kinase Regulation and GTPase Signaling
- Orbital Angular Momentum in Optics
- Single-cell and spatial transcriptomics
- Angiogenesis and VEGF in Cancer
- PI3K/AKT/mTOR signaling in cancer
- Pancreatic function and diabetes
- Erythrocyte Function and Pathophysiology
- Micro and Nano Robotics
- Escherichia coli research studies
- Vibrio bacteria research studies
- Modular Robots and Swarm Intelligence
- Microtubule and mitosis dynamics
- Phagocytosis and Immune Regulation
University of Saskatchewan
2005-2024
Saskatchewan Cancer Agency
2004-2024
University of Toronto
2013-2023
Toronto Public Health
2017
Occupational Cancer Research Centre
2015
University of Regina
2002
The phosphatidylinositol 3-kinase (PI3K) signaling pathway is deregulated in many human diseases including cancer, diabetes, obesity, and autoimmunity. PI3K consists of a p110 catalytic protein p85alpha regulatory protein, required for the stabilization localization p110-PI3K activity. enzyme generates key lipid 3,4,5-trisphosphate, which dephosphorylated by PI3-phosphatase PTEN. Here we show another function protein: it binds directly to enhances PTEN phosphatase We demonstrate that...
We introduce the microfluidic organoids for drug screening (MODS) platform, a digital system that is capable of generating arrays individually addressable, free-floating, three-dimensional hydrogel-based microtissues (or 'organoids'). Here, we focused on liver organoids, driven by need early-stage methods hepatotoxicity enable "fail early, fail cheaply" strategy in discovery. demonstrate hepatic can be formed from co-cultures HepG2 and NIH-3T3 cells embedded hydrogel matrices. The exhibit...
Portable digital microfluidic serological immunoassays for measles and rubella were developed evaluated in a remote setting.
Abstract We introduce Digital microfluidic Isolation of Single Cells for -Omics (DISCO), a platform that allows users to select particular cells interest from limited initial sample size and connects single-cell sequencing data their immunofluorescence-based phenotypes. Specifically, DISCO combines digital microfluidics, laser cell lysis, artificial intelligence-driven image processing collect the contents single heterogeneous populations, followed by analysis genomes transcriptomes...
We report a new technique called Digital microfluidic Immunocytochemistry in Single Cells (DISC). DISC automates protocols for cell culture, stimulation and immunocytochemistry, enabling the interrogation of protein phosphorylation on pulsing with stimulus as little 3 s. was used to probe states platelet-derived growth factor receptor (PDGFR) downstream signalling protein, Akt, evaluate concentration- time-dependent effects stimulation. The high time resolution allowed surprising...
Microrobotics extends the reach of human-controlled machines to submillimeter dimensions. We introduce a microrobot that relies on optoelectronic tweezers (OET) is straightforward manufacture, can take nearly any desirable shape or form, and be programmed carry out sophisticated, multiaxis operations. One particularly useful program serial combination "load," "transport," "deliver," which applied manipulate wide range micrometer-dimension payloads. Importantly, microrobots in this manner are...
Abstract There is great interest in the development of micromotors which can convert energy to motion sub-millimeter dimensions. Micromachines take micromotor concept a step further, comprising complex systems multiple components work concert effectively realize mechanical tasks. Here we introduce light-driven and micromachines that rely on optoelectronic tweezers (OET). Using circular micro-gear as unit component, demonstrate range new functionalities, including touchless micro-feed-roller...
Highly sensitive and reproducible analysis of samples containing low amounts protein is restricted by sample loss the introduction contaminants during processing. Here, we report an All-in-One digital microfluidic (DMF) pipeline for proteomic reduction, alkylation, digestion, isotopic labeling analysis. The system features end-to-end automation, with integrated thermal control optimized droplet additives manipulation analysis, automated interface to liquid chromatography tandem mass...
Digital microfluidics (DMF), a fluid-handling technique in which picolitre-microlitre droplets are manipulated electrostatically on an array of electrodes, has recently become popular for applications chemistry and biology. DMF devices reconfigurable, have no moving parts, compatible with conventional high-throughput screening infrastructure (e.g., multiwell plate readers). For these other reasons, digital been touted as being potentially useful new tool multiplexed screening. Here, we...
Rab5 and Rab4 are small monomeric GTPases localized on early endosomes function in vesicle fusion events. These Rab proteins regulate the endocytosis recycling or degradation of activated receptor tyrosine kinases such as platelet-derived growth factor (PDGFR). The p85alpha subunit phosphatidylinositol 3'-kinase contains a BH domain with sequence homology to GTPase activating (GAPs), but has not previously been shown possess GAP activity. In this report, we demonstrate that activity toward...
We report a digital microfluidic system designed for droplet-based long-term culture and analysis of algae. The includes unique innovations relative to standard devices including an active reservoir structure maintain homogeneous cell density, customized device layout capable controlling wide range different droplet volumes, vertical interconnects collect spent reagents, detection zones compatible with parallel-scale optical measurements using multiwell plate reader, optimized features...
Optical micromanipulation has become popular for a wide range of applications. In this work, new type optical platform, patterned optoelectronic tweezers (p-OET), is introduced. p-OET devices, the photoconductive layer (that continuous in conventional OET device) patterned, forming regions which electrode locally exposed. It demonstrated that micropatterns are useful repelling unwanted particles/cells, and also keeping selected particles/cells place after turning off light source, minimizing...
An emerging tool to better recapitulate the complexity of tumor biology in vitro is 3D culture models. Here, we describe a free-floating collagen-based hydrogel system with embedded cancer cells, called microtissues. The microtissues are based on well-established modular tissue engineering method. They mimic natural development microenvironment, features such as hypoxia and treatment resistance. To demonstrate utility model system, triple negative breast cells were cultured using this method...
Adding bone marrow-derived mesenchymal stromal cells (bmMSCs) to endothelialized collagen gel modules resulted in mature vessel formation, presumably caused part by the observed display of pericyte-like behavior for transplanted GFP(+) bmMSCs. A previous study determined that rat aortic endothelial (RAECs) delivered on surface small (∼0.8 mm long×0.5 diameter) cylinders (microtissues, modular tissue engineering) formed vessels after transplantation into immunosuppressed Sprague-Dawley (SD)...
Immunoprecipitation (IP) is a common method for isolating targeted protein from complex sample such as blood, serum, or cell lysate. In particular, IP often used the primary means of target purification analysis by mass spectrometry novel biologically derived pharmaceuticals, with particular utility identification molecules bound to target. Unfortunately, labor-intensive technique, difficult perform in parallel, and has limited options automation. Furthermore, technique typically large...
A digital microfluidic technique allows for analysis of sub-populations invading cells by transcriptomics.
Rab proteins regulate vesicle fusion events during the endocytosis, recycling, and degradation of activated receptor tyrosine kinases. The p85α subunit phosphatidylinositol 3-kinase has GTPase-activating protein activity toward Rab5 Rab4, an severely reduced by a single point mutation (p85-R274A). Expression p85-R274A resulted in increased platelet-derived growth factor (PDGFR) activation downstream signaling (Akt MAPK) decreased PDGFR degradation. We now report that biological consequences...
Pre-concentration by liquid intact paper (P-CLIP) is a new technique that allows digital microfluidics to handle large volumes of dilute analyte.
Summary Aeromonas hydrophila secretes protein toxins via the type II pathway, involving products of at least two operons, exeAB ( gspAB ) and exeC‐N gspC‐N ). In studies reported here, aerolysin secretion was restored to C5.84, an exeA ::Tn 5 –751 mutant, by overexpression exeD alone in trans . Expression indicated that these results did not reflect a role ExeAB regulation operon. Instead, immunoblot analysis showed ExeD multimerize fractionation membranes monomeric remained inner membrane....
Modular tissue engineering is a means of building functional, vascularized tissues using small (∼1 mm long×0.5 diameter) components. While this approach being explored for its utility in adipose and cardiac islet transplantation, the initial question study was to assess fate endothelial cells (EC) after transplantation delivered on surface modules, without an embedded cell. Rat aortic EC-covered collagen gel modules were transplanted into omental pouch allogeneic (outbred) Sprague-Dawley...