A5018600439- Sepsis Diagnosis and Treatment
- Respiratory Support and Mechanisms
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Long-Term Effects of COVID-19
- Single-cell and spatial transcriptomics
- Intensive Care Unit Cognitive Disorders
- Nosocomial Infections in ICU
- Neonatal Respiratory Health Research
- Heart Rate Variability and Autonomic Control
- Machine Learning in Healthcare
- S100 Proteins and Annexins
- Fatty Acid Research and Health
- Adolescent and Pediatric Healthcare
- Pharmacological Receptor Mechanisms and Effects
- Chronic Obstructive Pulmonary Disease (COPD) Research
- COVID-19 Impact on Reproduction
- Thermal Regulation in Medicine
- Immune cells in cancer
- Pancreatitis Pathology and Treatment
- Healthcare Policy and Management
- Peptidase Inhibition and Analysis
- Galectins and Cancer Biology
- Antimicrobial Peptides and Activities
Swedish Medical Center
2020-2023
University of Washington
2001-2023
Swedish Medical Center
2017-2023
St. Joseph Health System
2020
Providence College
2020
Harborview Medical Center
2012-2017
Pulmonary and Critical Care Associates
2013
Seattle University
2006
We present an integrated analysis of the clinical measurements, immune cells, and plasma multi-omics 139 COVID-19 patients representing all levels disease severity, from serial blood draws collected during first week infection following diagnosis. identify a major shift between mild moderate disease, at which point elevated inflammatory signaling is accompanied by loss specific classes metabolites metabolic processes. Within this stressed environment multiple unusual cell phenotypes emerge...
Toll-like receptor (TLR) 2 and TLR4 play important roles in the early, innate immune response to microbial challenge. TLR2 is preferentially involved inflammatory lipoteichoic acid, lipopeptides, glycans from a variety of microbes, whereas essential for complete LPSs. We report here that transduces phenol-soluble modulin, factor secreted by Staphylococcus epidermidis. The TLR2-mediated this modulin was enhanced TLR6 but inhibited TLR1, indicating functional interaction between these...
Human Toll-like receptors (TLRs) comprise a family of proteins that recognizes pathogen-associated molecular patterns (PAMPs) and initiates host innate immune responses.Neutrophils, monocytes, macrophages are critical cellular components the human system.Proinflammatory cytokines, such as granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage (GM-CSF), macrophage (M-CSF), interferon-γ (IFN-γ), have been shown to up-regulate microbicidal activity in these effector cells...
To characterize the use of and disposition from a tertiary pediatric emergency department (PED) by children with chronic conditions varying degrees medical complexity.We conducted retrospective cohort study using dataset all registered PED patient visits at Seattle Children's Hospital January 1, 2008, through December 31, 2009. complexity was classified validated algorithm (Clinical Risk Group software) into nonchronic conditions: episodic chronic, lifelong progressive malignancy. Outcomes...
Introduction Common variants in genes related to inflammation, innate immunity, epithelial cell function, and angiogenesis have been reported be associated with risks for Acute Lung Injury (ALI) outcomes. We tested whether previously-reported associations can validated an independent cohort at risk ALI. Methods identified 37 genetic 27 previously ALI prepared allelic discrimination assays 12 SNPs from 11 MAF>0.05 genotyped these Caucasian subjects a of critically ill patients meeting...
Improving the prospective identification of patients with systemic inflammatory response syndrome (SIRS) and sepsis at low risk for organ dysfunction death is a major clinical challenge.To develop validate multibiomarker-based prediction model 28-day mortality in critically ill SIRS sepsis.A derivation cohort (n = 888) internal test 278) were taken from study intensive care unit (ICU) meeting two four criteria an academic medical center whom plasma was obtained within 24 hours. The...
Multiple organ dysfunction syndrome (MODS) is a common complication of sepsis in mechanically ventilated patients with acute respiratory distress syndrome, but the links between mechanical ventilation and MODS are unclear. Our goal was to determine whether minimally injurious strategy synergizes low-dose endotoxemia induce activation pro-inflammatory pathways lungs systemic circulation, resulting distal and/or injury.We administered intraperitoneal Escherichia coli lipopolysaccharide (LPS; 1...
BackgroundSevere COVID-19 is associated with innate immunopathology, and CD14, a proximal activator of immunity, has been suggested as potential therapeutic target.MethodsWe conducted the anti-CD14 Treatment Trial (CaTT), Phase II randomized, double-blind, placebo-controlled trial at 5 US-sites between April 12, 2021 November 30, (NCT04391309). Hospitalized adults requiring supplemental oxygen (<30 LPM) were randomized 1:1 to receive 4 daily doses intravenous IC14, an monoclonal antibody, or...
Abstract Modulins represent microbial products that stimulate cytokine production in host cells. The modulins responsible for gram-positive sepsis remain poorly understood. Staphylococci release a factor (or factors) activates nuclear factor-κB and stimulates cells of macrophage lineage. This factor, termed phenol-soluble modulin (PSM), has been recently isolated from culture supernatant Staphylococcus epidermidis. We examined the effects PSM on proinflammatory properties human neutrophils...
Section:ChooseTop of pageAbstract <<Material and MethodsResultsDiscussionReferencesCITING ARTICLES
IMPORTANCE: The Surviving Sepsis Campaign recommends standard operating procedures for patients with sepsis. Real-world evidence about sepsis order set implementation is limited. OBJECTIVES: To estimate the effect of usage on hospital mortality. DESIGN: Retrospective cohort study. SETTING AND PARTICIPANTS: Fifty-four acute care hospitals in United States from December 1, 2020 to November 30, 2022 involving 104,662 hospitalized MAIN OUTCOMES MEASURES: Hospital RESULTS: was used 58,091 (55.5%)...
Mitogen-activated protein kinase 1 (MAP3K1) regulates numerous intracellular signaling pathways involved in inflammation and apoptosis. We hypothesized that genetic variation MAP3K1 might be associated with outcomes patients acute respiratory distress syndrome (ARDS), these variants would alter MAP3K1-mediated changes transcriptional regulation. To test this hypothesis, we genotyped single-nucleotide polymorphisms covering linkage disequilibrium bins 306 subjects ARDS from the ARDSNet FACTT...
SUMMARY Host immune responses play central roles in controlling SARS-CoV2 infection, yet remain incompletely characterized and understood. Here, we present a comprehensive response map spanning 454 proteins 847 metabolites plasma integrated with single-cell multi-omic assays of PBMCs which whole transcriptome, 192 surface proteins, T B cell receptor sequence were co-analyzed within the context clinical measures from 50 COVID19 patient samples. Our study reveals novel cellular subpopulations,...
Background Sepsis is a life-threatening condition that can rapidly lead to organ damage and death. Existing risk scores predict outcomes for patients who have already become acutely ill. Objective We aimed develop model identifying at of getting sepsis within 2 years in order support the reduction morbidity mortality. Methods Machine learning was applied 2,683,049 electronic health records (EHRs) with over 64 million encounters across five states models predicting patient’s years. Features...
<sec> <title>BACKGROUND</title> Sepsis is a life-threatening condition that can rapidly lead to organ damage and death. Existing risk scores predict outcomes for patients who have already become acutely ill. </sec> <title>OBJECTIVE</title> We aimed develop model identifying at of getting sepsis within 2 years in order support the reduction morbidity mortality. <title>METHODS</title> Machine learning was applied 2,683,049 electronic health records (EHRs) with over 64 million encounters across...