A5037957654- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Immunotherapy and Immune Responses
- Long-Term Effects of COVID-19
- Refrigeration and Air Conditioning Technologies
- Gut microbiota and health
- Probiotics and Fermented Foods
- Tryptophan and brain disorders
- Microfluidic and Capillary Electrophoresis Applications
- Adipose Tissue and Metabolism
- RNA Research and Splicing
- COVID-19 Clinical Research Studies
- Erythrocyte Function and Pathophysiology
- Respiratory Support and Mechanisms
- Extracellular vesicles in disease
- Diabetes and associated disorders
- T-cell and B-cell Immunology
- Nanofabrication and Lithography Techniques
- Cancer-related gene regulation
Institute for Systems Biology
2015-2025
Systems Biology Institute
2025
Seattle University
2004-2022
InSysBio (Russia)
2020-2022
North Seattle College
2013-2015
University of Maryland, College Park
1999-2002
Post-acute sequelae of COVID-19 (PASC) represent an emerging global crisis. However, quantifiable risk factors for PASC and their biological associations are poorly resolved. We executed a deep multi-omic, longitudinal investigation 309 patients from initial diagnosis to convalescence (2-3 months later), integrated with clinical data patient-reported symptoms. resolved four PASC-anticipating at the time diagnosis: type 2 diabetes, SARS-CoV-2 RNAemia, Epstein-Barr virus viremia, specific...
We present an integrated analysis of the clinical measurements, immune cells, and plasma multi-omics 139 COVID-19 patients representing all levels disease severity, from serial blood draws collected during first week infection following diagnosis. identify a major shift between mild moderate disease, at which point elevated inflammatory signaling is accompanied by loss specific classes metabolites metabolic processes. Within this stressed environment multiple unusual cell phenotypes emerge...
Exosomes are endosome-derived membrane vesicles carrying proteins and nucleic acids that involved in cellular functions such as intercellular communication, protein RNA secretion, antigen presentation. Therefore, exosomes serve potential biomarkers for many diseases including cancer. Because difficult to enrich or purify from biofluids, quantification of is tedious inaccurate. Here, we present a real-time, label-free, quantitative method detect characterize tumor-derived without enrichment...
Objective Programmed cell death protein 1 (PD-1) checkpoint inhibition and adoptive cellular therapy have had limited success in patients with microsatellite stable colorectal cancer liver metastases (CRLM). We sought to evaluate the effect of interleukin 10 (IL-10) blockade on endogenous T chimeric antigen receptor (CAR-T) antitumour function CRLM slice cultures. Design created organotypic cultures from human (n=38 patients’ tumours) tested effects a neutralising antibody against IL-10...
Surface plasmon resonance imaging (SPRi) is a label-free technique used for the quantitation of binding affinities and concentrations wide variety target molecules. Although SPRi capable determining constants multiple ligands in parallel, current commercial instruments are limited to single analyte stream on ligand spots. Measurement kinetics requires serial introduction different concentrations; such repeated experiments conducted manually therefore time-intensive. To address these...
The field of nutrition has evolved rapidly over the past century. Nutrition scientists and policy makers in developed world have shifted focus their efforts from dealing with diseases overt nutrient deficiency to a new paradigm aimed at coping conditions excess-calories, sedentary lifestyles stress. Advances science, technology manufacturing largely eradicated diseases, while simultaneously facing growing challenges obesity, non-communicable aging. research gone through necessary evolution,...
DNA arrays are valuable tools in molecular biology laboratories. Their rapid acceptance was aided by the release of plans for a pin-spotting microarrayer researchers at Stanford. Inkjet microarraying is flexible, complementary technique that allows synthesis any oligonucleotide sequences de novo. We describe here an open-source inkjet arrayer capable rapidly producing sets unique 9,800-feature arrays.
The detection and quantification of specific proteins in complex mixtures is a major challenge for proteomics. For example, the development disease-related biomarker panels will require fast efficient methods obtaining multiparameter protein profiles. We established high throughput, label-free method analyzing serum using surface plasmon resonance imaging antibody microarrays. Microarrays were fabricated standard pin spotting on bare gold substrates, samples applied binding analysis...
We discuss here a new approach to detecting hepatotoxicity by employing concentration changes of liver-specific blood proteins during disease progression.These are capable assessing the behaviors their cognate liver biological networks for toxicity or perturbations.Blood biomarkers highly desirable diagnostics as is easily accessible and baths virtually all organs.Fifteen were identified markers acetaminophen (APAP)-induced using three proteomic technologies: label-free antibody microarrays,...
Peptides are excellent biointerface molecules and diagnostic probes with many advantages such as good penetration, short turnover time, low cost. We report here an efficient peptide screening strategy based on in situ single bead sequencing a microarray. Two novel peptides YLFFVFER (H6) KLRLEWNR (H10) specifically binding to the tumor biomarker human epidermal growth factor receptor 2 (HER2) aKD of 10–8 M were obtained from 105 library. Conjugated nanoparticles, both H6 H10 showed specific...
The application scope of surface plasmon resonance (SPR) and SPR imaging (SPRi) is rapidly growing, tools such as high-performance low-cost slides could enable more rapid growth the field. We describe herein a novel silver slide, addressing inherent instability plain structure by improving adhesion between glass substrate layer with thin buffer gold. Covered self-assembled monolayer (SAM) only, characteristics slide remain steady for than 3 months under regular storage. In bioassay,...
Elucidating the relationships between a class I peptide antigen, CD8 T cell receptor (TCR) specific to that and phenotype emerges following antigen stimulation, remains mostly unsolved problem, largely due lack of large data sets can be mined resolve such relationships. Here, we describe Antigen-TCR Pairing Multiomic Analysis T-cells (APMAT), an integrated experimental-computational framework designed for high-throughput capture analysis cells, with paired TCR sequence, single-cell...
Elucidating the relationships between a class I peptide antigen, CD8 T cell receptor (TCR) specific to that and phenotype emerges following antigen stimulation, remains mostly unsolved problem, largely due lack of large data sets can be mined resolve such relationships. Here, we describe Antigen-TCR Pairing Multiomic Analysis T-cells (APMAT), an integrated experimental-computational framework designed for high-throughput capture analysis cells, with paired TCR sequence, single-cell...
Peptide ligands as targeting probes for in vivo imaging and drug delivery have attracted great interest the biomedical community. However, high affinity specificity screening of large peptide libraries remains a tedious process. Here, we report continuous-flow microfluidic method one-bead-one-compound (OBOC) combinatorial library screening. We screened with 2 × 10(5) beads within 4 h discovered 140 noncanonical hits tumor marker, aminopeptidase N (APN). Using Clustal algorithm, identified...
Aging manifests as progressive deteriorations in homeostasis, requiring systems-level perspectives to investigate the gradual molecular dysregulation of underlying biological processes. Here, we report systemic changes regulation processes under multiple lifespan-extending interventions. Differential Rank Conservation (DIRAC) analyses mouse liver proteomics and transcriptomics data show that mechanistically distinct interventions (acarbose, 17α-estradiol, rapamycin, calorie restriction)...
Metastatic colorectal cancer (CRC) is a major cause of cancer-related death, and incidence rising in younger populations (younger than 50 years). Current chemotherapies can achieve response rates above 50%, but immunotherapies have limited value for patients with microsatellite-stable (MSS) cancers. The present study investigates the impact chemotherapy on tumor immune microenvironment. We treat human liver metastases slices 5-fluorouracil (5-FU) plus either irinotecan or oxaliplatin, then...
The anemias of myelodysplastic syndrome (MDS) and Diamond Blackfan anemia (DBA) are generally macrocytic always reflect ineffective erythropoiesis yet result from diverse genetic mutations. To delineate shared mechanisms that lead to cell death, we studied the fate single erythroid marrow cells individuals with DBA or MDS-5q. We defined an unhealthy (vs healthy) differentiation trajectory using transcriptional pseudotime surface proteins. trajectories diverge immediately after upregulate...
Maximum pressures developed by the respiratory muscles can indicate health of system, help to determine maximum flow rates, and contribute power development. Past measurements have been found be inadequate for inclusion in some exercise models involving respiration.Maximum inspiratory expiratory airway were measured over a range lung volumes 29 female 19 male adults. A commercial bell spirometry system was programmed occlude airflow at nine target ranging from 10% 90% vital capacity.In...
We present a microfluidic synthesizer made entirely of Teflon material for solid phase peptide synthesis (SPPS). Solvent-resistant perfluoroalkoxy (PFA) was used to construct chip-sized devices featuring multiple tri-layer pneumatic microvalves. Using these devices, model peptides were automatically synthesized and cleaved in situ continuous-flow manner. The total coupling cleavage time significantly reduced compared conventional bulk reactors. decapeptide, instance, took less than 6 h using...
Respirator inspiratory resistance can affect performance times, especially when the experiment is optimized to elicit respiratory stress. Twelve subjects performed on a treadmill at constant speeds and grades chosen result in times of 5-15 min. Six levels were used, ranging from 0.78 7.64 cm H2O.sec/L. The results showed that decrease linearly with level, no threshold value apparent. Inspiratory also induces hypoventilation, lower minute volumes oxygen consumption values higher resistances....
The airflow perturbation device (APD) is an instrument for the measurement of respiratory resistance. APD small, lightweight, fast and requires no special breathing manoeuvres. Airflow determines resistance by superimposing a periodic signal onto spontaneous with variable device. Respiratory impedance ratio magnitude pressure to flow perturbation, in-phase portion impedance. was tested determine its responses repeated measurements changes in A mechanical model test showed that could detect...