A5087387911- Chronic Obstructive Pulmonary Disease (COPD) Research
- Microfluidic and Capillary Electrophoresis Applications
- Pediatric health and respiratory diseases
- Asthma and respiratory diseases
- Respiratory Support and Mechanisms
- Innovative Microfluidic and Catalytic Techniques Innovation
- COVID-19 Clinical Research Studies
- Inhalation and Respiratory Drug Delivery
- Immune cells in cancer
- Analytical Chemistry and Sensors
- Molecular Junctions and Nanostructures
- Electrochemical Analysis and Applications
- Neonatal Respiratory Health Research
- 3D Printing in Biomedical Research
- Electrowetting and Microfluidic Technologies
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Respiratory viral infections research
- Mechanical Failure Analysis and Simulation
- Oral Health Pathology and Treatment
- Fire Detection and Safety Systems
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Wnt/β-catenin signaling in development and cancer
- SARS-CoV-2 detection and testing
Anhui University
2025
Children's Hospital of Zhejiang University
2024
University of British Columbia
2007-2022
St. Paul's Hospital
2019-2022
St. Paul's Hospital
2019-2022
Providence Health Care
2020
CRRC (China)
2020
British Heart Foundation
2019
Canada's Michael Smith Genome Sciences Centre
2010-2017
Southern Medical University
2017
Surface plasmon resonance imaging (SPRi) is a label-free technique used for the quantitation of binding affinities and concentrations wide variety target molecules. Although SPRi capable determining constants multiple ligands in parallel, current commercial instruments are limited to single analyte stream on ligand spots. Measurement kinetics requires serial introduction different concentrations; such repeated experiments conducted manually therefore time-intensive. To address these...
Abstract The classical M1/M2 polarity of macrophages may not be applicable to inflammatory lung diseases including chronic obstructive pulmonary disease (COPD) due the complex microenvironment in lungs and plasticity macrophages. We examined macrophage sub-phenotypes bronchoalveolar lavage (BAL) fluid 25 participants with CD40 (a M1 marker) CD163 M2 marker). Of these, we performed RNA-sequencing on each subtype 10 patients using Illumina NextSeq 500. Approximately 25% did harbor or surface...
Asthma is a chronic respiratory disorder marked by airway hyperresponsiveness and inflammation, yet the specific molecular mechanisms driving these processes remain only partially understood. This study aims to better understand how JAK2/STAT3/EPAS1 axis regulates inflammation ferroptosis in asthma. Asthma-related datasets were retrieved from Gene Expression Omnibus (GEO) database, differentially expressed genes (DEGs) identified. Weighted Co-expression Network Analysis (WGCNA) was used...
Rationale: Inhaled corticosteroids (ICS) are commonly prescribed with long-acting β2-agonists (LABA) in chronic obstructive pulmonary disease (COPD). To date, the effects of ICS therapy on airway microbiome COPD unknown. Objectives: determine ICS/LABA patients COPD. Methods: Clinically stable were enrolled into a 4-week run-in period during which was discontinued and all participants placed formoterol (Form) 12 μg twice daily (BID). The then randomized to budesonide/formoterol (Bud + Form;...
Genome-wide association studies have linked gene variants of the receptor patched homolog 1 (PTCH1) with chronic obstructive pulmonary disease (COPD). However, its biological role in is unclear. Our objective was to determine expression pattern and PTCH1 lungs patients COPD. Airway epithelial-specific protein epithelial morphology were assessed lung tissues control COPD patients. mRNA measured bronchial cells obtained from individuals without The effects siRNA knockdown on repair mucous...
The effect of air-borne nanoparticles (NPs) on human health is an active area research, with clinical relevance evidenced by the current COVID-19 pandemic. As in vitro models for such studies, lung-on-a-chip (LOAC) devices can represent key physical and physiological aspects alveolar tissues. However, widespread adoption LOAC device NP testing has been hampered low intra-laboratory inter-laboratory reproducibility. To complement ongoing experimental work, we carried out finite-element...
As the field of microfluidics continues to grow, there is an increasing demand for public education about this technology. This article presents a quick, simple, safe, and inexpensive method teaching younger students general public. (To listen podcast article, please go Analytical Chemistry multimedia page at pubs.acs.org/page/ancham/audio/index.html .).
Lung macrophages are the key immune effector cells in pathogenesis of Chronic Obstructive Pulmonary Disease (COPD). Several studies have shown an increase their numbers bronchoalveolar lavage fluid (BAL) subjects with COPD compared to controls, suggesting a pathogenic role disease initiation and progression. Although reduced lung macrophage phagocytic ability has been previously COPD, relationship between macrophages’ phenotypic characteristics functional properties is still unclear. (1)...
Abstract Background People with human immunodeficiency virus (PWH) have an increased risk of developing chronic obstructive pulmonary disease (COPD). Methods We phenotyped lung macrophages in 4 subgroups—M1 (CD40+CD163−), M2 (CD40−CD163+), double positives (CD40+CD163+), and negatives (CD40−CD163−)—and we determined their phagocytic capacity PWH without COPD. Results COPD more double-negative (84.1%) versus (54.3%) controls (23.9%) (P=.004) reduced phagocytosis (P=.012). Double-negative had...
We present a novel bonding technique for poly(dimethylsiloxane) (PDMS)-based devices employing chemical surface modifications at room temperature. PDMS surfaces were functionalized to primary amine groups, and glass or gold substrates carboxylic acid groups. Irreversible was achieved by bringing the two in contact reacting temperature form peptide bonds between substrates. Shear tests reveal bond strengths be comparable values obtained using conventional methods. also describe use of...
In surface-based biosensors, the nonspecific or undesired adsorption of probe is an important characteristic that typically difficult to measure and therefore control eliminate. A methodology for measuring then minimizing eliminating this problem on gold surfaces, readily applicable many common surface modifications presented. Combining electrochemical perturbation fluorescence microscopy, we show potential at which adsorbed species removed can be used as estimate strength adsorbate-surface...
Patients with chronic obstructive pulmonary disease (COPD) are commonly treated inhaled corticosteroid/long-acting ß2-agonist combination therapy. While previous studies have investigated the host-microbiome interactions in COPD, effects of specific steroid formulations on this complex cross-talk remain obscure.We collected and evaluated data from Study to Investigate Differential Effects Inhaled Symbicort Advair Lung Microbiota (DISARM), a randomized controlled trial. Bronchoscopy was...
Acne vulgaris is a very common skin disorder. Previous studies have indicated that genetic background factors play key roles in the onset of acne. Our previous investigation implicated several genes androgen metabolism pathway with acne Han Chinese population. Thus, we further investigated and variants important this for their relationship pathology In study, total 610 subjects, including 403 patients 207 healthy controls, were genotyped 15 single-nucleotide polymorphisms...
The receptor for advanced glycation end products (RAGE) is a cell surface molecule of the immunoglobulin superfamily that binds diverse endogenous ligands involved in development chronic diseases and inflammatory damage. A growing body evidence has suggested RAGE progression obstructive pulmonary disease (COPD). present study investigated existence an association among three polymorphisms (-374T/A, -429T/C, G82S) gene with risk COPD Chinese population. genotypes were determined by polymerase...
Epidemiological studies have shown that female smokers are at higher risk of chronic obstructive pulmonary disease (COPD). Female patients worse symptoms and health status increased exacerbations. We determined the differences in transcriptome airway epithelium between males females, as well sex-by-smoking interaction. processed public gene expression data human into a discovery cohort 211 subjects (never n = 68; current 143) two replication cohorts 104 (21 never, 52 current, 31 former...
The associations between airway eosinophilia, measured in sputum or peripheral blood, and acute exacerbations of chronic obstructive pulmonary disease (AECOPD) are inconsistent. We therefore aimed to determine the association eosinophilia bronchoalveolar lavage (BAL) fluid AECOPD a clinical cohort. analyzed differential cell counts from baseline BAL participants DISARM trial (Clinicaltrials.gov #NCT02833480) classified by presence absence (>1% total leukocytes). determined over 1 year...
Integrated sensing and biosensing microfluidic systems often require sealing between polysiloxane, glass, gold interfaces, while maintaining functional support on the surface within cell chamber (e.g., biomolecular interaction analysis). A carboxylated trimethoxysilane (TMS-EDTA) coating has been shown to facilitate bonding of polydimethylsiloxane (PDMS) glass slides. In this work, adsorption TMS-EDTA onto Au is characterized in order enable its rational use hybrid glass/gold substrates....