A5000134747- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- Nuclear Receptors and Signaling
- Epigenetics and DNA Methylation
- 14-3-3 protein interactions
- Immune cells in cancer
- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- MicroRNA in disease regulation
- Cell Image Analysis Techniques
- Histone Deacetylase Inhibitors Research
- Cytomegalovirus and herpesvirus research
- Melanoma and MAPK Pathways
- vaccines and immunoinformatics approaches
- Immune Response and Inflammation
- Receptor Mechanisms and Signaling
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Macrophage Migration Inhibitory Factor
Suzhou Institute of Systems Medicine
2018-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2018-2024
California Institute of Technology
2018-2022
Academy of Medical Sciences
2022
Pasadena City College
2018
University of California, Irvine
2009-2016
The University of Texas Health Science Center at San Antonio
2014
University of California System
2011
Xiamen University
2006-2008
National University of Singapore
2008
By diversifying antibody biological effector functions, class switch DNA recombination has a central role in the maturation of response. Here we show that BCR-signalling synergizes with Toll-like receptor (TLR) signalling to induce recombination. activates non-canonical NF-κB pathway and enhances TLR-dependent canonical pathway, thereby inducing activation-induced cytidine deaminase (AID), which is critical for Escherichia coli lipopolysaccharide (LPS) triggers dual TLR4/BCR-signalling...
The accumulation of acidic metabolic waste products within the tumor microenvironment inhibits effector functions tumor-infiltrating lymphocytes (TILs). However, it remains unclear how an environment affects T cell metabolism and differentiation. Here we show that prolonged exposure to acid reprograms intracellular mitochondrial fitness preserves stemness. Mechanistically, elevated extracellular acidosis impairs methionine uptake via downregulation SLC7A5, therefore altering H3K27me3...
Adoptive T cell transfer, in particular TCR therapy, holds great promise for cancer immunotherapy with encouraging clinical results. However, finding the right clone is a tedious, time-consuming, and costly process. Thus, there critical need single technologies to conduct fast multiplexed functional analyses followed by recovery of interest. Here, we use droplet microfluidics screening real-time monitoring activation upon recognition target tumor cells. Notably, our platform includes...
Abstract Class-switch DNA recombination (CSR) and somatic hypermutation (SHM), which require activation-induced cytidine deaminase (AID), plasma cell differentiation, requires B lymphocyte–induced maturation protein-1 (Blimp-1), are critical for the generation of class-switched hypermutated (mature) Ab autoantibody responses. We show that histone deacetylase inhibitors valproic acid butyrate dampened AICDA/Aicda (AID) PRDM1/Prdm1 (Blimp-1) mRNAs by upregulating miR-155, miR-181b, miR-361 to...
The determination of individual cell trajectories through a high-dimensional cell-state space is an outstanding challenge for understanding biological changes ranging from cellular differentiation to epigenetic responses diseased cells upon drugging. We integrate experiments and theory determine the that single BRAF
The acidic metabolic byproducts within the tumor microenvironment (TME) hinder T cell effector functions. However, their effects on infiltration remain largely unexplored. Leveraging comprehensive Cancer Genome Atlas dataset, we pinpoint 16 genes that correlate with extracellular acidification and establish a metric known as "tumor acidity (TuAci) score" for individual patients. We consistently observe negative association between TuAci score lymphocyte (T score) across various human cancer...
RAD51 recombinase activity plays a critical role for cancer cell proliferation and survival, often contributes to drug-resistance. Abnormally elevated function hyperactive homologous recombination (HR) rates have been found in panel of cancers, including breast chronic myeloid leukaemia (CML). Directly targeting attenuating the deregulated has therefore proposed as an alternative supplementary strategy treatment. Here we show that newly identified small molecule, IBR2, disrupts...
Despite extensive studies on the antitumor properties of berberine, a small molecule derived from Coptidis rhizoma (Huanglian in Chinese) and many other plants, underlying mechanism remains poorly understood. Here, we found that berberine-induced cell apoptosis human gastric cancer cells with increase expression level cleaved poly ADP-ribose polymerase caspase-3, impairment mitochondrial membrane potential (Δψm) berberine-treated cells. In our further studies, results demonstrated...
High expression in cancer 1 (Hec1) is an oncogene overly expressed many human cancers. Small molecule inhibitor of Nek2/Hec1 (INH) targeting the Hec1 and its regulator, Nek2, mitotic pathway, was identified to inactivate Hec1/Nek2 function mediated by protein degradation that subsequently leads chromosome mis-segregation cell death. To further improve efficacy INH, a series INH analogues were designed, synthesized, evaluated. Among these 33 newly synthesized analogues, three them, 6, 13, 21,...
Class-switch DNA recombination (CSR) is central to the antibody response, in that it changes immunoglobulin heavy chain (IgH) constant region, thereby diversifying biological effector functions of antibodies. The activation-induced cytidine deaminase (AID)-centered CSR machinery excises and rejoins between an upstream (donor) a downstream (acceptor) S which precede respective region DNA. AID stabilized on regions by 14-3-3 adaptors. These adaptors display high affinity for 5'-AGCT-3'...
By diversifying the biological effector functions of antibodies, class switch DNA recombination (CSR) plays a critical role in maturation immune response. It is initiated by activation-induced cytidine deaminase (AID)-mediated deoxycytosine deamination, yielding deoxyuridine (dU), and dU glycosylation uracil glycosylase (Ung) antibody (S) region DNA. Here we showed that translesion synthesis polymerase Rev1 directly interacted with Ung targeted an AID-dependent Ung-independent fashion S...