A5070087498- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- Cytokine Signaling Pathways and Interactions
- DNA Repair Mechanisms
- Immune Response and Inflammation
- RNA modifications and cancer
- Cytomegalovirus and herpesvirus research
- 14-3-3 protein interactions
- CAR-T cell therapy research
- Hepatitis B Virus Studies
- Cancer-related molecular mechanisms research
- Hepatitis C virus research
- Endoplasmic Reticulum Stress and Disease
- IL-33, ST2, and ILC Pathways
- Protein Degradation and Inhibitors
- Histone Deacetylase Inhibitors Research
- Glycosylation and Glycoproteins Research
- Fungal and yeast genetics research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- NF-κB Signaling Pathways
The University of Texas at San Antonio
2015-2024
The University of Texas Health Science Center at Houston
2017-2024
The University of Texas Health Science Center at San Antonio
2014-2024
Mays Cancer Center at UT Health San Antonio
2021
Central South University
2020
University of California, Irvine
2005-2013
University of California System
2007
California Institute of Technology
2005
University of Southern California
1996-2004
Southern California University for Professional Studies
2004
The basal core promoter (BCP) of hepatitis B virus (HBV) controls the transcription both precore RNA and RNA. codes for secreted e antigen, while major protein DNA polymerase also is pregenomic double mutation nucleotides 1762 1764 in BCP from A G to T A, respectively, frequently observed HBV sequences isolated chronic patients. Several papers have reported conflicting results regarding whether this important antigen expression. In order address issue, we introduced into genome studied its...
By diversifying antibody biological effector functions, class switch DNA recombination has a central role in the maturation of response. Here we show that BCR-signalling synergizes with Toll-like receptor (TLR) signalling to induce recombination. activates non-canonical NF-κB pathway and enhances TLR-dependent canonical pathway, thereby inducing activation-induced cytidine deaminase (AID), which is critical for Escherichia coli lipopolysaccharide (LPS) triggers dual TLR4/BCR-signalling...
Humanized mice are limited in terms of modeling human immunity, particularly with regards to antibody responses. Here we constructed a humanized (THX) mouse by grafting non-γ-irradiated, genetically myeloablated Kit
Sirt1 mediates epigenetic modulation of B cell AID expression and antibody response by deacetylating histones, Dnmt1 NF-κB p65.
Abstract Of all gynecologic cancers, epithelial-ovarian cancer (OCa) stands out with the highest mortality rates. Despite efforts, 90% of individuals who receive standard surgical and cytotoxic therapy experience disease recurrence. The precise mechanism by which leukemia inhibitory factor (LIF) its receptor (LIFR) contribute to progression OCa remains unknown. Analysis databases revealed that elevated expression LIF or LIFR was associated poor progression-free survival patients a predictor...
ABSTRACT Hepatitis B virus (HBV) X gene encodes a multifunctional protein that can regulate cellular signaling pathways, interact with transcription factors, and induce hepatocellular oncogenesis. In spite of its diverse activities, the precise role in viral life cycle HBV remains unclear. To investigate this question, we have produced transgenic mice carry either wild-type genome or mutated incapable expressing 16.5-kDa protein. Our results indicate while is not absolutely essential for...
Estrogen enhances antibody and autoantibody responses through yet to be defined mechanisms. It has been suggested that estrogen up-regulates the expression of activation-induced cytosine deaminase (AID), which is critical for class switch DNA recombination (CSR) somatic hypermutation (SHM), direct activation this gene. AID, as we have shown, induced by HoxC4 homeodomain transcription factor, binds a conserved HoxC4/Oct site in AICDA/Aicda promoter. Here show estrogen-estrogen receptor (ER)...
Ig class switch DNA recombination (CSR) in B cells is crucial to the maturation of antibody responses. It requires IgH germline IH-CH transcription and expression AID, both which are induced by engagement CD40 or dual a Toll-like receptor (TLR) cell (BCR). Here, we have addressed cross-regulation between two different TLRs TLR CSR induction using stimulation system involving lipopolysaccharides (LPS). LPS-mediated long-term primary class-switched responses memory-like vivo generation plasma...
The relationship between B cells and CD4 T has been carefully studied, revealing a collaborative effort in which promote the activation, differentiation, expansion of while so-called "helper" provide signals to cells, influencing their class switching fate. Interactions CD8 are not as well although exhibit an accelerated contraction after certain infections B-cell-deficient mice. Here, we find that significantly enhance primary cell responses vaccination. Moreover, memory numbers function...
Maturation of antibody responses entails somatic hypermutation (SHM), class-switch DNA recombination (CSR), plasma cell differentiation, and generation memory B cells, it is thought to require T help. We showed that Toll-like receptor 4 (TLR4)–B (BCR) (receptor for antigen) coengagement by 4-hydroxy-3-nitrophenyl acetyl (NP)–lipopolysaccharide (LPS) ( Escherichia coli lipid A polysaccharide O-antigen) or TLR5-BCR Salmonella flagellin induces mature NP in Tcr β −/− δ NSG/B mice. TLR-BCR...
Class-switch DNA recombination (CSR) is central to the antibody response, in that it changes immunoglobulin heavy chain (IgH) constant region, thereby diversifying biological effector functions of antibodies. The activation-induced cytidine deaminase (AID)-centered CSR machinery excises and rejoins between an upstream (donor) a downstream (acceptor) S which precede respective region DNA. AID stabilized on regions by 14-3-3 adaptors. These adaptors display high affinity for 5'-AGCT-3'...
Triple-negative breast cancer (TNBC) has a poor clinical outcome, due to lack of actionable therapeutic targets. Herein we define lysosomal acid lipase A (LIPA) as viable molecular target in TNBC and identify stereospecific small molecule (ERX-41) that binds LIPA. ERX-41 induces endoplasmic reticulum (ER) stress resulting cell death, this effect is on evidenced by specific LIPA mutations providing resistance. Importantly, demonstrate activity independent function but dependent its ER...
Hepatitis C virus (HCV) F protein is a newly discovered HCV gene product that expressed by translational ribosomal frameshift. Little known about the biological properties of this protein. By performing pulse-chase labeling experiments, we demonstrate here labile with half-life <10 min in Huh7 hepatoma cells and vitro. The could be substantially increased proteasome inhibitors, suggesting rapid degradation mediated pathway. Further immunofluorescence staining subcellular fractionation...
By diversifying the biological effector functions of antibodies, class switch DNA recombination (CSR) plays a critical role in maturation immune response. It is initiated by activation-induced cytidine deaminase (AID)-mediated deoxycytosine deamination, yielding deoxyuridine (dU), and dU glycosylation uracil glycosylase (Ung) antibody (S) region DNA. Here we showed that translesion synthesis polymerase Rev1 directly interacted with Ung targeted an AID-dependent Ung-independent fashion S...
Ribosomes can be programmed to shift from one reading frame another during translation. Hepatitis C virus (HCV) uses such a mechanism produce F protein the −2/+1 frame. We now report that HCV frameshift signal mediate synthesis of core zero frame, and 1.5-kDa −1/+2 This triple decoding function does not require sequences flanking is apparently independent membranes polyprotein. Two consensus −1 in type 1 facilitate ribosomal frameshifts into both overlapping frames. A sequence which located...