A5082072941- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- Infective Endocarditis Diagnosis and Management
- Cancer therapeutics and mechanisms
- Pharmaceutical and Antibiotic Environmental Impacts
- Streptococcal Infections and Treatments
- Crystallization and Solubility Studies
- Tuberculosis Research and Epidemiology
- Bioactive Compounds and Antitumor Agents
- Crystallography and molecular interactions
- Antibiotics Pharmacokinetics and Efficacy
- Escherichia coli research studies
- X-ray Diffraction in Crystallography
- Photochemistry and Electron Transfer Studies
- Viral gastroenteritis research and epidemiology
- Retinoids in leukemia and cellular processes
- Endodontics and Root Canal Treatments
- Metal Extraction and Bioleaching
- Listeria monocytogenes in Food Safety
- Magnetism in coordination complexes
- Leprosy Research and Treatment
- Legionella and Acanthamoeba research
- Advanced biosensing and bioanalysis techniques
Hokkaido University
2018-2024
National Institute of Infectious Diseases
2020-2024
New York University
2020
National Institute Of Hygiene And Epidemiology
2020
National Hospital of Pediatrics
2020
Jikei University School of Medicine
2016
Osaka City University
2015
Graduate School USA
2015
Abstract The World Health Organization has listed C . jejuni as one of 12 microorganisms on a global priority list for antibiotic resistance due to rapid increase in strains resistant fluoroquinolone antibiotics. This is conferred through single point mutation the QRDR region within gyrA gene known be involved DNA supercoiling. We have previously revealed that changes supercoilikng play major role regulation virulence with relaxation supercoiling associated increased attachment and invasion...
Macrolide-resistant Bordetella pertussis emerged in Vietnam during 2016-2017. Direct analyses of swab samples from 10 patients with revealed a macrolide-resistant mutation, A2047G, the 23S rRNA. We identified MT104 genotype B. (which is prevalent mainland China) and its variants these patients.
Bordetella pertussis, the causative agent of whooping cough, can cause pertussis outbreaks in humans, especially school-aged children. Here, we performed whole-genome sequencing 51 B. isolates (epidemic strain MT27) collected from patients infected during 6 school-associated lasting less than 4 months. We compared their genetic diversity with that 28 sporadic (non-outbreak MT27 isolates) based on single-nucleotide polymorphisms (SNPs). Our temporal SNP analysis revealed a mean accumulation...
Although many drug-resistant nontyphoidal Salmonella (NTS) infections are reported globally, their treatment is challenging owing to the ineffectiveness of currently available antimicrobial drugs against resistant bacteria. It therefore essential discover novel for management these infections. In this study, we report high inhibitory activities fluoroquinolones (FQs; WQ-3810 and WQ-3334) with substitutions at positions R-1 by 6-amino-3,5-difluoropyridine-2-yl R-8 methyl group or bromine,...
ABSTRACT Quinolone-resistant nontyphoidal Salmonella , one of the prominent pathogens causing acute gastroenteritis, has become a public health concern globally. The World Health Organization ranked fluoroquinolone-resistant as high-priority pathogen for researching and developing new antibiotics. WQ-3034 WQ-3154 are relatively synthetic fluoroquinolones with distinctive structures. 6-amino-3,5-difluoropyridine-2-yl at R 1 3-hydroxyazetidinyl 7 addition chlorine atom 8 . similar basic...
The inhibitory effect of WQ-3810 on DNA gyrase was assayed to evaluate the potential as a candidate drug for treatment quinolone resistant Salmonella Typhymurium infection. WQ-3810, ciprofloxacin and nalidixic acid compared by accessing concentration that halves enzyme activity (IC50) purified S. Typhimurium wildtype mutant with amino substitution at position 83 or/and 87 in subunit A (GyrA) causing resistance. As result, reduced both lower than acid. Remarkably, showed higher substitutions...
Aims: Quinolone-resistant nontyphoidal Salmonella having serine replaced by isoleucine at the 83rd amino acid in GyrA (GyrA-Ser83Ile) has recently been found Asian countries. In this study, we aimed to examine direct effect of substitution Ser83Ile on DNA gyrase activity and/or resistance quinolones. Materials and Methods: Using 50% maximal inhibitory concentrations (IC50s) quinolones, recombinant wild type (WT) seven mutant gyrases substitutions, including Ser83Ile, were screened for...
We describe a case of bacteremia in human immunodeficiency virus-infected patient caused by Bordetella pertussis strain lacking 2 major virulence factors, filamentous hemagglutinin and fimbriae. Although B is uncommon, physicians should be aware that even attenuated strains can cause invasive infection immunocompromised patients. gram-negative coccobacillus causes severe paroxysmal coughing disease known as whooping cough or pertussis. colonizes the epithelial cells respiratory tract,...
We have studied the relationship between π-topology and photo-excited high-spin states of π-conjugated spin systems, 9-anthracen-(3-phenyl-<italic>t</italic>-butylnitroxide) radical (<bold>1m</bold>) 9-anthracen-(4-phenyl-<italic>t</italic>-butylnitroxide) (<bold>1p</bold>) by time-resolved ESR transient absorption spectroscopies.
Quinolones show excellent antibacterial activity against Salmonella isolates. Recently, however, quinolone resistance has been increasing in bacteria. This study aimed to examine vitro, and compare the of DC-159a enterica serovar Typhimurium with that ciprofloxacin nalidixic acid. Inhibitory effects quinolones were assessed by drug concentration needed inhibit supercoiling recombinant DNA gyrases 50% (IC50). Dilution methods used determine minimum inhibitory (MIC) two different strains,...
Abstract Multilocus variable-number tandem repeat analysis (MLVA) is widely used for genotyping of Bordetella pertussis , the causative bacteria pertussis. However, MLVA losing its discriminate power because prevalence epidemic MT27 strain (MLVA-27) increasing worldwide. To address this, we developed a single nucleotide polymorphism (SNP) method based on multiplexed single-base extension (SBE) assay. A total 237 isolates collected in Japan during 1999–2018 were genotyped and classified into...
Many pregnant women take vitamin supplements during pregnancy. The aim of this paper was to clarify the effects dietary supplementation prior and/or pregnancy on child behavior.A prospective birth cohort study from 3 years age involving 1271 pairs Japanese and their newborns, carried out. completed a self-administered questionnaire third trimester To evaluate deviations in behavior as an endpoint, each mother Child Behavior Checklist for ages 2-3 after birth. Participant characteristics were...
Abstract Autoagglutination (Agg) of Bordetella pertussis is often observed in clinical laboratory. However, its causal factors and frequency circulating strains are unknown. Repeated single colony isolation enabled us to detect an Agg - mutant the supernatant + strain B. . Whole-genome sequencing immunoblot analysis disclosed that had a C-deletion fim3 promoter region (Pfim3) which abolished Fim3 fimbriae production. A -knock out also lacked phenotype. isolates were detected higher...
Aims: WQ-3810 has strong inhibitory activity against Salmonella and other fluoroquinolone-resistant pathogens. The unique potentiality of this is attributed to 6-amino-3,5-difluoropyridine-2-yl at R1 group. aim study was examine its derivatives WQ-3334 WQ-4065 as the new drug candidate for wild-type that carrying QnrB19. Materials Methods: half maximal concentrations (IC50s) WQ-3810, (Br atom in place methyl group R8), (6-ethylamino-3,5-difluoropyridine-2-yl R1) presence or absence QnrB19...
Abstract We evaluated the genetic diversity of Bordetella pertussis , causative agent pertussis, within households by whole‐genome sequencing. In pairwise comparisons 23 isolates collected from 11 households, single‐nucleotide polymorphism (SNP) analysis revealed extremely low SNP (≤1 SNP) between isolate pairs: no SNPs were detected in 10 and one was obtained remaining household. This uncommon for B. resulted a nonsynonymous substitution (Ala303Thr) nicotinate phosphoribosyltransferase....
The emergence of macrolide-resistant Bordetella pertussis (MRBP) is a significant problem because it reduces treatment options for and exacerbates the severity spread disease. MRBP has been widely prevalent in mainland China since 2010s sporadically detected other Asian countries. In Japan, two clinical strains were first isolated Tokyo Osaka between June July 2018. isolates BP616 BP625 harbored same virulence-associated allelic genes (including ptxP1 , ptxA1 prn1 fim3A fhaB3 ) MT195...
Quinolone-resistant
We report the complete genome sequence of macrolide-resistant Bordetella pertussis BP616, which was first isolated in 2018 Japan. The BP616 can serve as a valuable specific reference for genomic and epidemiological studies this resistant bacterium.
Background: Leprosy is a chronic infectious disease caused by Mycobacterium leprae and the treatment of choice ofloxacin (OFX). Specific amino acid substitutions in DNA gyrase M. have been reported leading to resistance against drug. In our previous study, WQ-3810, fluoroquinolone with new R1 group (6-amino-3,5-difluoropyridin-2-yl) was shown strong inhibitory activity on OFX-resistant gyrases leprae, structural characteristics its predicted enhance activity. Methodology/Principal Finding:...