A5021245467- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Mechanisms of cancer metastasis
- Renal and related cancers
- Bone health and treatments
- Galectins and Cancer Biology
- Bone Metabolism and Diseases
- Cancer-related molecular mechanisms research
- Healthcare and Venom Research
- Bone health and osteoporosis research
- Complementary and Alternative Medicine Studies
- Connective tissue disorders research
- Scoliosis diagnosis and treatment
- Maternal and fetal healthcare
- Spine and Intervertebral Disc Pathology
- Neurogenetic and Muscular Disorders Research
- Pregnancy-related medical research
- Maternal and Perinatal Health Interventions
- Eicosanoids and Hypertension Pharmacology
- Bone and Joint Diseases
- Chronic Kidney Disease and Diabetes
Osaka University
1992-2023
Osaka University Hospital
2018-2019
Osaka City General Hospital
2004
Osaka City University
1996
microRNAs (miRNAs) stably exist in circulating blood and are encapsulated extracellular vesicles such as exosomes. The aims of this study were to identify which exosomal miRNAs highly produced from epithelial ovarian cancer (EOC) cells, analyze whether serum miRNA can be used discriminate patients with EOC healthy volunteers, investigate the functional role progression.Exosomes collected culture media serous cell lines, namely TYK-nu HeyA8 cells. An microarray revealed that several including...
microRNAs (miRNAs) stably exist in circulating blood encapsulated extracellular vesicles such as exosomes; therefore, serum miRNAs have the potential to serve novel cancer biomarkers. New diagnostic markers detect high grade serous ovarian (HGSOC) are urgently needed. The aim of this study was identify specific HGSOC and analyze whether miRNA can discriminate patients from healthy controls or with malignancies other histological types.Exosomes cell lines were collected exosomal extracted....
Paclitaxel is a first-line drug for treating epithelial ovarian cancer (EOC). However, prognosis patients with advanced stage remains poor due to primary or acquired resistance. Therefore, overcoming chemoresistance one of the greatest challenges in EOC. In this study, we identified microRNAs (miRNA) that regulate paclitaxel resistance and tested their potential utility as therapeutic targets. Paclitaxel-resistant cell lines were established using two EOC lines: SKVO3ip1 HeyA8. miRNA PCR...
Abstract Although bevacizumab (BEV) plays a key role in ovarian cancer treatment, BEV resistance is often observed clinical settings. This study aimed to identify the genes responsible for resistance. C57BL/6 mice inoculated with ID-8 murine cells were treated anti-VEGFA antibody or IgG (control) twice weekly 4 weeks. The sacrificed, then, RNA was extracted from disseminated tumors. qRT-PCR assays performed angiogenesis-related and miRNAs that altered by treatment. SERPINE1/PAI-1 found be...
Abstract Paclitaxel resistance is a critical challenge in ovarian cancer treatment. This study aimed to identify microRNAs (miRNAs) that modulate paclitaxel for use as potential therapeutic targets such settings. Paclitaxel-resistant cell lines were established using two lines: SKOV3ip1 and HeyA8. The evaluation of miRNA polymerase chain reaction (PCR) arrays indicated the expression miR-522-3p was downregulated paclitaxel-resistant cells. restoration sensitized resistant cells paclitaxel,...
Glutathione is one of the most abundant thiols in mammalian tissues and plays important roles defense mechanism detoxification various metabolites, such as reactive xenobiotics free radicals. Nitric oxide (NO) readily reacts with thiol compounds, thereby generating chemically stable S-nitrosothiols. Although endotoxin has been known to induce NO synthase organs, particularly liver spleen, enhances production NO, correlation between glutathione metabolism endotoxemic subjects remains be...
Abstract Background Preoperative anemia affects perioperative outcomes and often causes fatigue psychological disorders. Therefore, should be treated before a patient undergoes surgery. Ninjin’yoeito (NYT), Japanese Kampo medicine composed of ginseng angelica root with the other 10 herbs, is administered for anemia, anxiety; however, there are few reports that have prospectively examined effects NYT surgery gynecological diseases. Hence, we tended to investigate its efficacy safety. Methods...
Denosumab is a major treatment option for patients with postmenopausal osteoporosis; however, the evidence its use lacking. Therefore, in this 24-month retrospective study, we aimed to evaluate effects of switching from minodronate (MIN) denosumab these patients.Patients osteoporosis either switched MIN (Group 1; n = 32) or continued 2; 24). Bone mineral density (BMD) lumbar spine (L2-L4) and femoral neck was assessed at baseline every 6 months 24 months. Serum bone-specific alkaline...
Although microalbuminuria has been suggested as an independent risk factor for ischemic heart disease, the relationship between diabetic nephropathy and macroangiopathy remains unclear. Previously, we reported that coronary artery calcification detected by electron beam computed tomography (EBCT) could indicate degree of atherosclerosis in type 2 patients. In this study, examine association arterial aortic microalbuminuria.Two hundred fifty-six patients, including 177 patients (106 with...
A pregnant woman presented with acute upper abdominal pain and nausea at 15 weeks' gestation. She had a history of cesarean delivery for abruption after the removal Shirodkar cerclage that was placed because cervical shortening caused by conization. became again 14 months later. Ultrasonography revealed no significant findings, single intrauterine pregnancy positive fetal heart activity confirmed. An intestinal obstruction suspected radiography showed multiple air–fluid levels in colon. Over...
<p>Supplementary Figure 8 shows the representative images of immunohistochemical staining a human normal kidney for PAI-1 and CD31.</p>
<p>Supplementary Figure 6 shows that the transduction of miR-143-3p does not attenuate in vitro tube formation HUVECs incubated with culture media SKOV3ip1 cells.</p>
<p>Supplementary Figure 7 shows that the transduction of miR-143-3p did not affect tumor growth in a murine model ovarian cancer.</p>
<p>Supplementary Figure 5 shows thatVEGF induces in vitro tube formation HUVECs at a concentration of ≥ 3 ng/ml, whereas treatment with 100 µg/ml anti-VEGFA antibody attenuates the effects VEGF.</p>
<p>Supplementary Figure 4 shows that the knockdown of VEGFA significantly inhibited expression level miR-143-3p, followed by upregulation PAI secretion from OC cells.</p>
<p>Supplementary Figure 1 shows Kaplan–Meier plotter analysis of genes that were upregulated during anti-VEGFA treatment.</p>
<p>Supplementary Figure 3 shows the correlation analysis between miR-10a-5p expression and PAI-1 concentration.</p>
<p>Supplementary Figure 2 shows that PAI-1 enhanced in vitro tube formation not only the absence of anti-VEGFA antibody but also presence antibody.</p>
<p>Supplementary Figure 3 shows the correlation analysis between miR-10a-5p expression and PAI-1 concentration.</p>
<p>Supplementary Figure 4 shows that the knockdown of VEGFA significantly inhibited expression level miR-143-3p, followed by upregulation PAI secretion from OC cells.</p>
<p>Supplementary Figure 2 shows that PAI-1 enhanced in vitro tube formation not only the absence of anti-VEGFA antibody but also presence antibody.</p>