A5085657160- Protease and Inhibitor Mechanisms
- Protein purification and stability
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Analytical Chemistry and Chromatography
- Biosensors and Analytical Detection
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related molecular mechanisms research
- Banking stability, regulation, efficiency
- Protein Tyrosine Phosphatases
- Global Financial Crisis and Policies
- Extracellular vesicles in disease
- bioluminescence and chemiluminescence research
- Viral Infectious Diseases and Gene Expression in Insects
- Monetary Policy and Economic Impact
- Cytokine Signaling Pathways and Interactions
- Liver physiology and pathology
- Respiratory viral infections research
- Renal Transplantation Outcomes and Treatments
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
Osaka University
2015-2025
Museum of Japanese Art Yamato Bunkakan
2024
Tsukuba Medical Center Hospital
1997-2024
Astellas Pharma (Japan)
2015-2022
Molecular Oncology (United States)
2018-2021
Saiseikai Nakatsu Hospital
2009-2021
Moffitt Cancer Center
2018-2021
University of Toyama
2010-2018
Kyushu Institute of Technology
2017-2018
Bristol-Myers Squibb (Japan)
2018
Hepatic oval cells are considered to be facultative hepatic stem cells(HSCs) that differentiate into hepatocytes and cholangiocytes in severely injured liver. have also been implicated tumorigenesis. However, their nature origin remain elusive. To isolate characterize mouse cells, we searched for cell surface molecules expressed on analyzed at the single-cell level by flow cytometric analysis vitro colony formation assay. We demonstrate epithelial adhesion molecule (EpCAM) is both normal...
Epithelial ovarian cancer (EOC) cells metastasize within the peritoneal cavity and directly encounter human mesothelial (HPMC) as initial step of metastasis. The contact between single layer involves direct communications that modulate progression but mechanisms are unclear. One candidate mediating cell-cell is exosomes, 30-100 nm membrane vesicles endocytic origin, through transfer proteins, mRNAs, or microRNAs. Therefore, goal was to mechanistically characterize how EOC-derived exosomes...
microRNAs (miRNAs) stably exist in circulating blood and are encapsulated extracellular vesicles such as exosomes. The aims of this study were to identify which exosomal miRNAs highly produced from epithelial ovarian cancer (EOC) cells, analyze whether serum miRNA can be used discriminate patients with EOC healthy volunteers, investigate the functional role progression.Exosomes collected culture media serous cell lines, namely TYK-nu HeyA8 cells. An microarray revealed that several including...
Oncostatin M (OSM) is a member of the IL-6 family cytokines. Mice deficient in OSM receptor (OSMR -/- ) showed impaired liver regeneration with persistent parenchymal necrosis after carbon tetrachloride (CCl 4 exposure. The recovery mass from partial hepatectomy was also significantly delayed OSMR mice. In contrast to wildtype mice, CCl administration only marginally induced expression tissue inhibitor metalloproteinase (TIMP)-1 and TIMP-2 genes correlating increased gelatinase activity...
Hyaluronic acid (HA) has been implicated in the proliferation and metastasis of tumor cells. However, most previous studies were conducted on extracellular matrix or pericellular HA, role circulating HA vivo not studied. is rapidly cleared from bloodstream. The scavenger receptor Stabilin-2 (Stab2) considered a major clearance for HA. Here we report dramatic elevation levels Stab2-deficient mice without any overt phenotype. Surprisingly, B16F10 melanoma cells to lungs was markedly suppressed...
The Janus kinase (JAK) family of tyrosine kinases is associated with various cytokine receptors. JAK1 and JAK3 play particularly important roles in the immune response, their inhibition expected to provide targeted modulation. Several oral JAK inhibitors have recently been developed for treating autoimmune diseases, including rheumatoid arthritis (RA). Here, we investigated pharmacological effects peficitinib (formerly known as ASP015K), a novel, chemically synthesized inhibitor. We found...
A three-dimensional (3D) windshield display can driving information in the vicinity of objects driver's front scene. We propose a super multi-view that present wide depth range. The technique provides smooth motion parallax. Motion parallax is only physiological cue for perceiving depths 3D images displayed at far distances; these cannot be perceived by other cues such as vergence, binocular disparity, and accommodation. prototype system, which generates 36 viewing zones with horizontal...
microRNAs (miRNAs) stably exist in circulating blood encapsulated extracellular vesicles such as exosomes; therefore, serum miRNAs have the potential to serve novel cancer biomarkers. New diagnostic markers detect high grade serous ovarian (HGSOC) are urgently needed. The aim of this study was identify specific HGSOC and analyze whether miRNA can discriminate patients from healthy controls or with malignancies other histological types.Exosomes cell lines were collected exosomal extracted....
// Yasuto Kinose 1 , Kenjiro Sawada Koji Nakamura Ikuko Aska Toda Erika Nakatsuka Kae Hashimoto Seiji Mabuchi Kazuhiro Takahashi 2 Hirohisa Kurachi 3 Ernst Lengyel 4 and Tadashi Kimura Department of Obstetrics Gynecology, Osaka University Graduate School Medicine, Suita, Osaka, Japan Yamagata Faculty Yamagata, Medical Center Research Institute for Maternal Child Health, Izumi, Departments Gynecology/Section Gynecologic Oncology, Chicago, IL, USA Correspondence to: Sawada, email: Keywords :...
Ovarian cancer remains the most lethal gynecologic and new targeted molecular therapies against this miserable disease continue to be challenging. In study, we analyzed expressional patterns of Interleukin-6 (IL-6) its receptor (IL-6R) expression in ovarian tissues, evaluated impact these expressions on clinical outcomes patients, found that a high-level IL-6R but not IL-6 cells is an independent prognostic factor. vitro analyses using cell lines, while six (RMUG-S, RMG-1, OVISE, A2780,...
IntroductionSeveral antigen tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been developed worldwide, but their clinical utility has not well established. In this study, we evaluated the analytical and performance of QuickNavi™-COVID19 Ag, a newly test in Japan.MethodsThis prospective observational study was conducted at PCR center between October 7 December 5, 2020. The included patients were referred from local public health 89 primary care facilities. We...
The association of cigarette smoking with development hearing impairment (loss 30 dB at 1000 Hz and 40 4000 Hz) over a 5-year follow-up was studied in 1554 non–hearing-impaired Japanese male office workers who ranged age from to 59 years. After controlling for potential predictors impairment, the relative risk low-frequency compared never smokers 1.12 (95% confidence interval [CI], 0.57 2.17) ever-smokers, 1.21 CI, 0.65 2.25) current 1 20 cigarettes/day, 1.35 0.70 2.61) 21 1.82 0.98 3.38) 31...
We aimed to develop a far-red luminescence imaging technology for visualization of disease specific antigens on cell surfaces in living body. First, we conjugated fluorescent indocyanine derivative biotinylated Cypridina luciferase. This conjugate produced bimodal spectrum that has long-wavelength bioluminescence emission the region as result resonance energy transfer. To generate luminescent probe with targeting and capabilities tumors, then linked this an anti-human Dlk-1 monoclonal...
Abstract Photoimmunotherapy (PIT) is a new type of tumor‐specific treatment utilizing monoclonal antibody (mAb)‐photosensitizer conjugates and near‐infrared (NIR) light irradiation. One potential PIT target, the I transmembrane protein TROP2, expressed at high levels in many cancers, including pancreatic carcinoma (PC) cholangiocarcinoma (CC), which its expression correlated with poor prognosis tumor aggressiveness. In this study, we assessed efficacy newly developed humanized anti‐TROP2 mAb...
Abstract The clinical utility of antigen test using anterior nasal samples has not been well evaluated. We conducted a prospective study in drive-through testing site located at PCR center to evaluate the diagnostic performance QuickNavi-COVID19 Ag and compare degrees coughs or sneezes induction severity pain between collection nasopharyngeal collection. included total 862 participants, which 91.6% were symptomatic. median duration from symptom onset sample was 2.0 days. Fifty-one...
Ovarian clear cell carcinoma (OCCC), particularly advanced or recurrent settings, is generally resistant to platinum-based chemotherapy, warranting novel therapeutic strategies. Mutations in the phosphoinositide 3-kinase/protein kinase B/mechanistic target of rapamycin (PI3K/AKT/mTOR) pathway are frequently reported OCCC. Therefore, we hypothesized that PI3K/mTOR dual inhibitor, GSK458, and arsenic trioxide may exert synergistic anti-tumor effects on We investigated trioxide, combination...