A5022707362- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Heme Oxygenase-1 and Carbon Monoxide
- Legionella and Acanthamoeba research
- Cytomegalovirus and herpesvirus research
- Microbial Inactivation Methods
- Toxin Mechanisms and Immunotoxins
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Vitamin C and Antioxidants Research
- Plant and fungal interactions
- Vitamin K Research Studies
- Escherichia coli research studies
- Plant Pathogens and Fungal Diseases
- Dermatology and Skin Diseases
- Advancements in Transdermal Drug Delivery
- Microbial Natural Products and Biosynthesis
- Viral gastroenteritis research and epidemiology
Vaxess Technologies (United States)
2024
University of California, Berkeley
2013-2020
University of California Davis Medical Center
2009
Abstract Legionella pneumophila is an intracellular bacterial pathogen that the cause of a severe pneumonia in humans called Legionnaires’ disease. A key feature L. pathogenesis rapid influx neutrophils into lungs, which occurs response to signaling via IL-1R. Two distinct cytokines, IL-1α and IL-1β, can stimulate type I IL-1β produced upon activation cytosolic sensors inflammasomes detect vitro vivo. Surprisingly, we find no essential role for neutrophil recruitment lungs pneumophila....
Background The MIMIX platform is a novel microneedle array patch (MAP) characterized by slowly dissolving tips that deploy into the dermis following application. We describe safety, reactogenicity, tolerability and immunogenicity for MAP vaccination against influenza. Methodology trial was Phase 1, exploratory, first-in-human, parallel randomized, rater, participant, study analyst-blinded, placebo-controlled in Canada. Forty-five healthy participants (18 to 39 years of age, inclusive) were...
ABSTRACT Upon entry into the host cell cytosol, facultative intracellular pathogen Listeria monocytogenes coordinates expression of numerous essential virulence factors by allosteric binding glutathione (GSH) to Crp-Fnr family transcriptional regulator PrfA. Here, we report that robust gene can be recapitulated growing bacteria in a synthetic medium containing GSH or other chemical reducing agents. Bacteria grown under these conditions were 45-fold more virulent an acute murine infection...
ABSTRACT Through unknown mechanisms, the host cytosol restricts bacterial colonization; therefore, only professional cytosolic pathogens are adapted to colonize this environment. Listeria monocytogenes is a Gram-positive intracellular pathogen that highly of both phagocytic and nonphagocytic cells. To identify L. determinants survival, we designed executed novel screen isolate mutants with survival defects. Multiple identified in were defective for synthesis menaquinone (MK), an essential...
ABSTRACT Cholesterol-dependent cytolysins (CDCs) represent a family of homologous pore-forming proteins secreted by many Gram-positive bacterial pathogens. CDCs mediate membrane binding partly through conserved C-terminal undecapeptide, which contains single cysteine residue. While mutational changes to other residues in the undecapeptide typically have severe effects, mutation residue alanine has minor effects on overall protein function. Thus, role this highly reactive remains largely...
Significance Intracellular pathogens utilize specialized mechanisms for replicating within host cells while minimizing cytotoxicity. Misregulation of bacterial virulence factors that are responsible infection can cause cell death and the consequent loss virulence. The results presented here reveal translation a cytolysin is reduced during growth, due to formation an mRNA secondary structure in translational inhibition. coding domain occludes ribosome binding site regulates synthesis activity...
Endophytes are microorganisms that live within the intercellular space of almost all vascular plants. benefit their hosts by producing bioactive secondary metabolites prevent invasion pathogenic agents. Endophytic specifically kill plant‐pathogenic or other competing might also have cytotoxic effects in humans, and thus represent a potential source medicinal compounds. It is estimated 95% fungal endophytes remain undiscovered unexplored. In present study, were isolated from plants collected...
Abstract Legionella pneumophila is an intracellular bacterial pathogen that the cause of a severe pneumonia in humans called Legionnaires’ Disease. L. infects alveolar macrophages and its virulence requires type IV secretion system (T4SS) injects effector proteins into host cytosol. Macrophages distinguish infection with T4SS+ (virulent) T4SS- (avirulent) bacteria, mount several distinct immune responses to virulent bacteria. Virulent induces block protein synthesis partially induced by 7...