A5001155490- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- T-cell and B-cell Immunology
- Inflammatory mediators and NSAID effects
- Immune Cell Function and Interaction
- Atherosclerosis and Cardiovascular Diseases
- Listeria monocytogenes in Food Safety
- Asthma and respiratory diseases
- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- vaccines and immunoinformatics approaches
- Vitamin C and Antioxidants Research
- Microbial Inactivation Methods
- Essential Oils and Antimicrobial Activity
- Immunodeficiency and Autoimmune Disorders
- Vitamin K Research Studies
- Hematopoietic Stem Cell Transplantation
- Toxoplasma gondii Research Studies
- Cancer, Stress, Anesthesia, and Immune Response
- Chemokine receptors and signaling
- Zebrafish Biomedical Research Applications
University of Washington
2019-2024
University of Wisconsin–Madison
2017-2022
Cornell University
2014
New York State College of Veterinary Medicine
2014
The function of macrophages in vitro is linked to their metabolic rewiring. However, macrophage metabolism remains poorly characterized situ. Here, we used two-photon intensity and lifetime imaging autofluorescent coenzymes, nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) flavin (FAD), assess the wound microenvironment. Inhibiting glycolysis reduced NAD(P)H mean made intracellular redox state more oxidized, as indicated by optical ratio. We found that TNFα+ had lower were oxidized...
Beta-catenin signaling has recently been tied to the emergence of tolerogenic dendritic cells (DCs). In this article, we demonstrate a novel role for beta-catenin in directing DC subset development through IFN regulatory factor 8 (IRF8) activation. We found that splenic precursors express beta-catenin, and DCs from mice with CD11c-specific constitutive activation upregulated IRF8 targeting Irf8 promoter, leading vivo expansion IRF8-dependent CD8a+, plasmacytoid, CD103+ CD11b2 DCs....
ABSTRACT Through unknown mechanisms, the host cytosol restricts bacterial colonization; therefore, only professional cytosolic pathogens are adapted to colonize this environment. Listeria monocytogenes is a Gram-positive intracellular pathogen that highly of both phagocytic and nonphagocytic cells. To identify L. determinants survival, we designed executed novel screen isolate mutants with survival defects. Multiple identified in were defective for synthesis menaquinone (MK), an essential...
Abstract Nonsteroidal anti-inflammatory drugs (NSAIDs) inhibit cyclooxygenase (COX) activity and are commonly used for pain relief fever reduction. NSAIDs following childhood vaccinations cancer immunotherapies; however, how influence the development of immunity these therapies is unknown. We hypothesized that would modulate an immune response to Listeria monocytogenes–based immunotherapy. Treatment mice with nonspecific COX inhibitor indomethacin impaired generation cell-mediated immunity....
Invariant natural killer T (iNKT) cells are a conserved population of innate lymphocytes that interact with key antigen-presenting to modulate adaptive T-cell responses in ways can either promote protective immunity, or limit pathological immune activation. Understanding the immunological networks engaged by iNKT mediate these opposing functions is pre-requisite effectively using for therapeutic applications. Using human umbilical cord blood xenotransplantation model, we show here...
Abstract Humoral immunity depends upon long-lived, antibody-secreting plasma cells and memory B (MBCs). MBCs exhibit significant phenotypic functional heterogeneity. Upon homologous rechallenge, thought to be of germinal center (GC) origin rapidly form antibody secreting plasmablasts but rarely enter a new GC, while other, less differentiated secondary GCs, do not plasmablasts. These two populations therefore respond subsequent infection by generating epitopes recognized pre-existing serum...
Listeria monocytogenes is an intracellular bacterium that elicits robust CD8 + T-cell responses. Despite the ongoing development of L . -based platforms as cancer vaccines, our understanding how drives responses remains incomplete. One overarching hypothesis activation cytosolic innate pathways critical for immunity, strains are unable to access cytosol fail elicit and in fact inhibit optimal priming. Counterintuitively, however, known pathways, such inflammasome type I IFN, lead impaired...
Long-lived plasma cells are important for preventing infection by maintaining baseline antibody titers. However, the cues leading to cell differentiation remain unclear. In this article, we discuss recent work assessing role of affinity on differentiation.
Abstract Listeria monocytogenes is an intracellular bacterium that elicits robust CD8 + T-cell responses. Despite the ongoing development of L. -based platforms as cancer vaccines, our understanding how drives responses remains incomplete. One overarching hypothesis activation cytosolic innate pathways critical for immunity, strains are unable to access cytosol fail elicit and in fact inhibit optimal priming. Counterintuitively, however, known pathways, such inflammasome type I IFN, lead...
Abstract The effector functions of macrophages across the spectrum activation states in vitro are linked to profound metabolic rewiring. However, metabolism remains poorly characterized vivo . To assess changes intracellular their native inflammatory microenvironment, we employed two-photon fluorescence lifetime imaging microscopy (FLIM) coenzymes NAD(P)H and FAD. We found that pro-inflammatory was associated with a decrease optical redox ratio [NAD(P)H/(NAD(P)H+FAD)] relative pro-resolving...
Abstract The bacterial pathogen Listeria monocytogenes (Lm) is being developed as a cancer immunotherapeutic platform due to its ability elicit robust CD8+ T cell responses. Though the role of cytokines in Lm-stimulated immunity well studied, eicosanoids, lipid modulators inflammation, unclear. We previously demonstrated that cyclooxygenase-2 (COX-2) dependent prostaglandin E2 (PGE2) essential for optimal T-cell priming. Antigen specific cells and protective were decreased following either...