A5009390604- Health and Medical Research Impacts
- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Innovations in Medical Education
- Cardiac and Coronary Surgery Techniques
- Health Sciences Research and Education
- Advances in Oncology and Radiotherapy
- Respiratory viral infections research
- Academic Publishing and Open Access
- RNA modifications and cancer
- Healthcare cost, quality, practices
- Diversity and Career in Medicine
- Global Health Workforce Issues
- Cancer Research and Treatments
- Ethics in Clinical Research
- Cardiac Fibrosis and Remodeling
- Congenital Heart Disease Studies
- Global Health and Surgery
- Clinical Reasoning and Diagnostic Skills
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Primary Care and Health Outcomes
- Health Systems, Economic Evaluations, Quality of Life
- Pancreatic function and diabetes
University of Toronto
2018-2025
Hospital for Sick Children
2025
University of Southern California
2009
Argonaute (AGO) proteins associate with small RNAs to direct their effector function on complementary transcripts. The nematode Caenorhabditis elegans contains an expanded family of 19 functional AGO proteins, many which have not been fully characterized. In this work, we systematically analyzed every C. using CRISPR-Cas9 genome editing introduce GFP::3xFLAG tags. We characterized the expression patterns each throughout development, identified RNA binding complements, and determined effects...
The Caenorhabditis elegans genome encodes nineteen functional Argonaute proteins that use 22G-RNAs, 26G-RNAs, miRNAs or piRNAs to regulate target transcripts. Only one is essential under normal laboratory conditions: CSR-1. While CSR-1 has been studied widely, nearly all studies have overlooked the fact csr-1 locus two isoforms. These isoforms differ by an additional 163 amino acids present in N-terminus of CSR-1a. Using CRISPR-Cas9 editing introduce GFP::3xFLAG into long (CSR-1a) and short...
Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire regulatory pathways promote endogenous genes limit deleterious transcripts to maintain genome homeostasis. Here we show that conserved TRIM-NHL protein, NHL-2, plays an role in modulating chromatin meiotic chromosome organization. We uncover NHL-2 as co-factor both positively (CSR-1) negatively (HRDE-1) acting 22G-small RNA somatic...
The 2021 Annual Joint Meeting (AJM) and Young Investigators' Forum of the Canadian Society for Clinical Investigation / Société Canadienne de Recherches Clinique (CSCI/SCRC) Clinician Investigator Trainee Association Canada/Association des Cliniciens-Chercheurs en Formation du Canada (CITAC/ACCFC) was hosted virtually on November 14-16th, 2021. theme AJM "Communication, Collaboration, Tools Next Generation Scientists", emphasized lectures, panels interactive workshops designed to provide...
The C. elegans genome encodes nineteen functional Argonaute proteins that utilize 22G-RNAs, 26G-RNAs, miRNAs, or piRNAs to regulate their target transcripts. Only one is essential under normal laboratory conditions: CSR-1. While CSR-1 has been studied widely, nearly all studies have overlooked the fact csr-1 locus two isoforms. These isoforms differ by an additional 163 amino acids present in N-terminus of CSR-1a. Using CRISPR-Cas9 editing introduce GFP::3xFLAG into long (CSR-1a) and short...
Summary Argonaute (AGO) proteins associate with small RNAs to direct their effector function on complementary transcripts. The nematode C. elegans contains an expanded family of 19 functional AGO proteins, many which have not been fully characterized. In this work we systematically analyzed every AGO, using CRISPR-Cas9 genome editing introduce GFP::3xFLAG tags. We characterized the expression patterns each throughout development, identified RNA binding complements, and determined effects ago...
Purpose: Despite the impact of physician-scientists on scientific discovery and translational medicine, several reports have signalled their declining workforce, reduced funding, insufficient protected research time. Given paucity outcome data Canadian MD/PhD programs, this study presents a national portrait sociodemographic characteristics, training trajectories, productivity, satisfaction in trainees alumni from MD/MSc programs. Methods: Quantitative were collected survey launched 2021....
Purpose and Hypothesis: Despite the increasing number of donation after circulatory death (DCD) heart transplants over last decade, there is no promising cardioprotective agent to improve DCD function in this clinical setting. There some evidence that glucagon-like peptide (GLP)-1 (28-36), a metabolite insulinotropic GLP-1(7-36) incretin hormone, provides cytoprotection coronary vasculature without activating GLP-1 receptor. This study aims explore potential benefits GLP-1(28-36) on hearts...
Background: Children with critical congenital heart disease (CHD) often require complex open-heart surgery long aortic cross-clamp times (XC). Despite refinement in cardioplegia, ventricular dysfunction due to inadequate myocardial protection remains a major clinical challenge. Glucagon-like peptide-1 (GLP-1) has proven cardioprotective properties. This study characterizes the mechanisms of injury XC and investigates properties GLP-1(28-36), naturally occurring fragment GLP-1....
Background: The ubiquitous second messenger cyclic adenosine monophosphate (cAMP) plays a key role in post-myocardial infarction [MI] remodeling, influencing apoptosis, inflammation, fibrosis and metabolism. Unlike transmembrane activators of cAMP, soluble adenylyl cyclase (sAC) represents unique source intracellular mitochondrial metabolic sensor due to its ability regulate oxidative metabolism cell survival. However, for sAC post-MI remodeling was not known. Methods Results: Permanent LAD...
The 2024 Annual Joint Meeting (AJM) and Young Investigators’ Forum of the Canadian Society for Clinical Investigation (CSCI) Clinician Investigator Trainee Association Canada (CITAC) was held on April 11, 2024, in Vancouver, British Columbia. Hosted collaboration with University Columbia (UBC) International Congress Academic Medicine (ICAM), this meeting marked a significant opportunity clinician investigator trainees to present their research connect national international peers. event...
Over the past year, leadership of Clinician Investigator Trainee Association Canada (CITAC), alongside our MD+ trainees, had opportunity to further develop and implement strategic plan in response evolving medical landscape. We have dedicated efforts progression towards a post-pandemic environment, taken advantage lessons learned during coronavirus disease 2019 (COVID-19) health crisis focused on enhancing in-person career development opportunities for members
This has been a great first half of the year for CITAC-ACCFC (Clinician Investigator Trainee Association Canada/Association des cliniciens-chercheurs en formation du Canada)! We are looking forward to our new members joining us in fall and welcoming back previous after summer.
e14631 Background: Telomerase-interference (TI), a novel therapeutic strategy, exploits the high telomerase activity in prostate cancer by introducing mutated RNA (MT-Ter) that encodes toxic telomeres. Until now, TI has been tested targeting human tumor cells xenografted into immuno-deficient mice, an inadequate model for predicting efficacy and toxicity. We designed validated 2 new gene constructs specifically target murine (mTER), enabling study of preclinical mouse models are...
Over the past two years, physician-scientist trainees have persevered in face of evolving challenges presented by ongoing coronavirus disease 2019 (COVID-19) pandemic. Research and healthcare institutions across country continue to feel impacts public health emergency. As scientists physicians generate evidence inform prevention treatment COVID-19, all disciplines adapted changing conditions their education.
On behalf of the Clinical Investigator Trainee Association Canada (CITAC) Board Directors, I would like to extend an enthusiastic welcome our new MD+ trainee members! hope you soaked up all that summer had offer and are in good back-to-school spirits. A academic year is upon us, opportunities abound for Canadian physician scientist community.