A5101981404- Protein Kinase Regulation and GTPase Signaling
- 14-3-3 protein interactions
- Receptor Mechanisms and Signaling
- Microtubule and mitosis dynamics
- Pancreatic function and diabetes
- Contact Dermatitis and Allergies
- Electrolyte and hormonal disorders
- Ion Transport and Channel Regulation
- Ubiquitin and proteasome pathways
- Occupational exposure and asthma
- Astro and Planetary Science
- Obesity and Health Practices
- Nuclear Receptors and Signaling
- PI3K/AKT/mTOR signaling in cancer
- Histone Deacetylase Inhibitors Research
- Musicians’ Health and Performance
- Ocular Infections and Treatments
- Ion Channels and Receptors
- Global Public Health Policies and Epidemiology
- Hormonal Regulation and Hypertension
- Advanced Biosensing Techniques and Applications
- Neuroendocrine Tumor Research Advances
- Gout, Hyperuricemia, Uric Acid
- Protein Hydrolysis and Bioactive Peptides
- Antibiotics Pharmacokinetics and Efficacy
The University of Melbourne
2015-2019
Walter and Eliza Hall Institute of Medical Research
2009-2019
Springer Nature (Germany)
2014
University of California, San Francisco
2009-2014
San Francisco VA Medical Center
2013
University of Dundee
2002-2010
MRC Protein Phosphorylation and Ubiquitylation Unit
2007-2010
Medical Research Council
2009
Hanson Institute
2003-2005
South Australia Pathology
2003-2005
AS160 (Akt substrate of 160 kDa) and TBC1D1 are related RabGAPs (Rab GTPase-activating proteins) implicated in regulating the trafficking GLUT4 (glucose transporter 4) storage vesicles to cell surface. All animal species examined contain TBC1D1, whereas evolved with vertebrates. has two clusters phosphorylated residues, either side second PTB (phosphotyrosine-binding domain). Each cluster contains a 14-3-3-binding site. When AMPK (AMP-activated protein kinase) is activated HEK (human...
The pyroptotic cell death effector gasdermin D (GSDMD) is required for murine models of hereditary inflammasome-driven, IL-1β-dependent, autoinflammatory disease, making it an attractive therapeutic target. However, the importance GSDMD more common conditions mediated by pathological IL-1β activation, such as gout, remain unclear. In this study, we address whether and recently described inhibitor necrosulfonamide (NSA) contribute to monosodium urate (MSU) crystal-induced death, release,...
The 14-3-3 proteins play a central role in the regulation of cell growth, cycling, and apoptosis by modulating functional activities key signaling proteins. Through binding to phosphoserine motif, alters target sequestering them, relocalizing conformationally altering their activity, or promoting interaction with other These functions are facilitated by, if not dependent on, its dimeric structure. We now show that status is regulated site-specific serine phosphorylation. found...
Journal Article A randomized, observer‐blinded trial of twice vs. three times weekly narrowband ultraviolet B phototherapy for chronic plaque psoriasis Get access H. Cameron, Cameron Photobiology Unit, Department Dermatology, University Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, U.K Dr Heather Cameron. E‐mail: hcameronderm@hotmail.com Search other works by this author on: Oxford Academic Google Scholar R.S. Dawe, Dawe S. Yule, Yule J. Murphy, Murphy S.H. Ibbotson,...
We devised a strategy of 14-3-3 affinity capture and release, isotope differential (d(0)/d(4)) dimethyl labeling tryptic digests, phosphopeptide characterization to identify novel targets insulin/IGF1/phosphatidylinositol 3-kinase signaling. Notably four known insulin-regulated proteins (PFK-2, PRAS40, AS160, MYO1C) had high d(0)/d(4) values meaning that they were more highly represented among 14-3-3-binding from insulin-stimulated than unstimulated cells. Among candidates, insulin receptor...
Proteolytic processing of the amiloride-sensitive epithelial sodium channel (ENaC) by serine proteases is known to be important for activation. Inappropriate ENaC activation may contribute pathophysiology cystic fibrosis and could involved in retention pathogenesis arterial hypertension context renal disease. We hypothesized that addition proteases, cathepsin activate ENaC. Cathepsin belong group cysteine play a pathophysiological role inflammatory diseases. Under conditions, cathepsin-S...
Neuropeptide signaling at the cell surface is regulated by metalloendopeptidases, which degrade peptides in extracellular fluid, and β-arrestins, interact with G protein-coupled receptors (GPCRs) to mediate desensitization. β-Arrestins also recruit GPCRs mitogen-activated protein kinases endosomes allow internalized continue signaling, but mechanisms regulating endosomal are unknown. We report that endothelin-converting enzyme-1 (ECE-1) degrades substance P (SP) early of epithelial cells...
Abstract Sub-Neptunes—volatile-rich exoplanets smaller than Neptune—are intrinsically the most common type of planet known. However, formation and nature these objects, as well distinctions between subclasses (if any), remain unclear. Two powerful tools to tease out secrets worlds are measurements (i) atmospheric composition structure revealed by transit and/or eclipse spectroscopy, (ii) mass, radius, density photometry Doppler spectroscopy. Here, we present OrCAS, a survey better elucidate...
Protein kinase A (PKA) has long been recognized as playing a major role in many regulatory processes cells through its activation by the ubiquitous second messenger cAMP. We show here novel mode of PKA type II that is independent cAMP and is, instead, dependent on sphingosine. specifically activated sphingosine analog, dimethylsphingosine, but not sphingosine-1-phosphate or other lipids. Like cAMP, activates holoenzyme catalytic subunit alone, suggesting mediated subunits. However,...
Abstract Moxifloxacin, a broad-spectrum fluoroquinolone with the methoxy group at position 8 of quinolone structure that is believed to confer reduced phototoxicity, was investigated in 32 healthy human male volunteers by randomized double-blind placebo and positive control (lomefloxacin) phototest technique. A comparison pre- on-drug photosensitivity levels tested an irradiation monochromator using relevant sunlight wavelengths, failed demonstrate phototoxicity after administration either...
Activated G protein-coupled receptors (GPCRs) are phosphorylated and interact with β-arrestins, which mediate desensitization endocytosis. Endothelin-converting enzyme-1 (ECE-1) degrades neuropeptides in endosomes can promote recycling. Although endocytosis, dephosphorylation, recycling accepted mechanisms of receptor resensitization, a large proportion desensitized remain at the cell surface. We investigated whether reactivation noninternalized, (phosphorylated) mediates resensitization...
The protein phosphatase inhibitor calyculin A activates PKB/Akt to ~50% of the activity induced by insulin-like growth factor 1 (IGF1) in HeLa cells promoting an evident increased phosphorylation Ser473 despite apparent lack Thr308 PKB. Nevertheless, A-induced activation PKB seems be dependent on basal levels phosphorylation, since a PDK1-dependent mechanism is required for A-dependent using embryonic stem derived from PDK1 wild-type and knockout mice. Data shown suggest that was largely...
Sub-Neptunes - volatile-rich exoplanets smaller than Neptune are intrinsically the most common type of planet known. However, formation and nature these objects, as well distinctions between sub-classes (if any), remain unclear. Two powerful tools to tease out secrets worlds measurements (i) atmospheric composition structure revealed by transit and/or eclipse spectroscopy, (ii) mass, radius, density photometry Doppler spectroscopy. Here we present OrCAS, a survey better elucidate origins,...
Proteolytic channel activation is a unique feature of the epithelial sodium (ENaC) but not yet fully understood. The serine protease trypsin known to activate ENaC in vitro may be relevant vivo . In this study, we wanted investigate whether trypsin‐like IV, expressed several tissues, can human ENaC. Moreover, using site‐directed mutagenesis identify functionally cleavage sites γ‐subunit channel. Xenopus laevis oocyte expression system, monitored proteolytic currents and appearance γ‐ENaC...