A5079286738- Cytokine Signaling Pathways and Interactions
- 14-3-3 protein interactions
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Chronic Myeloid Leukemia Treatments
- Ubiquitin and proteasome pathways
- Immunotherapy and Immune Responses
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- Eosinophilic Disorders and Syndromes
- Protein Kinase Regulation and GTPase Signaling
- Viral Infectious Diseases and Gene Expression in Insects
- Chemokine receptors and signaling
- Asthma and respiratory diseases
- Respiratory and Cough-Related Research
- Microbial Natural Products and Biosynthesis
- Platelet Disorders and Treatments
- Allergic Rhinitis and Sensitization
- RNA and protein synthesis mechanisms
- Cell death mechanisms and regulation
- Morinda citrifolia extract uses
- Bioactive Compounds and Antitumor Agents
South Australia Pathology
2006-2022
Centre for Cancer Biology
2010-2022
University of South Australia
2015-2022
Hanson Institute
1996-2008
The University of Adelaide
2001
Baker Heart and Diabetes Institute
1999
The University of Tokyo
1997
Westmead Hospital
1990
University of Auckland
1990
The 14-3-3 proteins play a central role in the regulation of cell growth, cycling, and apoptosis by modulating functional activities key signaling proteins. Through binding to phosphoserine motif, alters target sequestering them, relocalizing conformationally altering their activity, or promoting interaction with other These functions are facilitated by, if not dependent on, its dimeric structure. We now show that status is regulated site-specific serine phosphorylation. found...
The vitronectin receptor (VnR) is one member of a subset cell adhesion receptors within the integrin supergene family which shares beta 3 subunit (IIIa). We show here that VnR absent from surface monocytes freshly isolated blood but expressed on these cells after period in vitro culture. Such cultured (macrophages) patient with type I Glanzmann's thrombasthenia, however, failed to express VnR. Instead, immunoprecipitation monoclonal antibody directed alpha chain (alpha v) revealed novel...
Pathological drug withdrawal syndrome is linked to accumulation of JAK2 phosphorylation in V617F myelofibrosis.
AbstractThe human interleukin-3 receptor (IL-3R) is a heterodimer that comprises an IL-3-specific α chain (IL-3Rα) and common β (βc) shared with the receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF) IL-5. These belong to cytokine superfamily, but they are structurally functionally more related each other thus make up distinct subfamily. Although activation of normal occurs only in presence ligand, underlying mechanisms not known. We show here IL-3 induces...
Report14 February 2022Open Access Source DataTransparent process Targeting human CALR-mutated MPN progenitors with a neoepitope-directed monoclonal antibody Denis Tvorogov orcid.org/0000-0002-2009-1347 Centre for Cancer Biology, SA Pathology and University of South Australia, Adelaide, SA, Australia Contribution: Conceptualization, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Visualization, Methodology, Writing - original draft, review & editing Search more...
The human interleukin 3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) receptors undergo covalent dimerization of the respective specific α chains with common β subunit (βc) in presence cognate ligand. We have now performed alanine substitutions individual Cys residues βc to identify involved their contribution activation IL-3, GM-CSF, IL-5 receptors. found that substitution Cys-86, Cys-91, Cys-96 but not Cys-100 or Cys-234 abrogated disulfide-linked IL-3 receptor...
Integrated cascades of protein tyrosine and serine/threonine phosphorylation play essential roles in transducing signals response to growth factors cytokines. How adaptor or scaffold proteins assemble signaling complexes through both phosphotyrosine phosphoserine/threonine residues regulate specific pathways biological responses is unclear. We show multiple cell types that endogenous 14-3-3zeta phosphorylated on Tyr(179) granulocyte macrophage colony-stimulating factor. Importantly, can...
The prolactin receptor (PrlR) is a member of the cytokine superfamily that lacks an intrinsic kinase domain and relies on cytoplasmic Jak tyrosine kinases to transduce signals. Prolactin-induced Jak2 activation consequent phosphorylation downstream signaling molecules have been studied, but PrlR serine or threonine residues has not reported. Here we describe novel interaction between phosphoserine/phosphothreonine-binding 14-3-3 proteins. This association mediated by KCST391WP motif, which...